Elemental formula annotation of polar and lipophilic metabolites using 13C, 15N and 34S isotope labelling, in combination with high-resolution mass spectrometry
Patrick Giavalisco,Yan Li,Annemarie Matthes,Aenne Eckhardt,Hans-Michael Hubberten,Holger Hesse,Shruthi Segu,Jan Hummel,Karin Köhl,Lothar Willmitzer +9 more
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TLDR
A comprehensive multi-isotope labelling-based strategy using fully labelled plant tissues, in combination with a fractionated metabolite extraction protocol, which can be applied in either an automated database-dependent or a database-independent analysis of the plant polar metabolome and lipidome.Abstract:
The unbiased and comprehensive analysis of metabolites in any organism presents a major challenge if proper peak annotation and unambiguous assignment of the biological origin of the peaks are required. Here we provide a comprehensive multi-isotope labelling-based strategy using fully labelled (13) C, (15) N and (34) S plant tissues, in combination with a fractionated metabolite extraction protocol. The extraction procedure allows for the simultaneous extraction of polar, semi-polar and hydrophobic metabolites, as well as for the extraction of proteins and starch. After labelling and extraction, the metabolites and lipids were analysed using a high-resolution mass spectrometer providing accurate MS and all-ion fragmentation data, providing an unambiguous readout for every detectable isotope-labelled peak. The isotope labelling assisted peak annotation process employed can be applied in either an automated database-dependent or a database-independent analysis of the plant polar metabolome and lipidome. As a proof of concept, the developed methods and technologies were applied and validated using Arabidopsis thaliana leaf and root extracts. Along with a large repository of assigned elemental compositions, which is provided, we show, using selected examples, the accuracy and reliability of the developed workflow.read more
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The endoplasmic reticulum pathway for membrane lipid synthesis has a significant contribution toward shoot removal-induced root chloroplast development in arabidopsis
TL;DR: In this paper, the root cells exhibit a higher proportion of glycolipid from the endoplasmic reticulum (ER) pathway, which suggests that the ER pathway plays a major role in guard cell chloroplast development.
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It takes two: Reciprocal scion-rootstock relationships enable salt tolerance in 'Hass' avocado
Silit Lazare,Hagai Yasuor,Uri Yermiyahu,Anastasiya Kuhalskaya,Yariv Brotman,Alon Ben-Gal,Arnon Dag +6 more
TL;DR: In this paper, the authors compared the ion, metabolite and lipid profiles of leaves, roots and trunk drillings of mature Hass avocado scion grafted onto two different rootstocks during gradual exposure to salinity.
Posted ContentDOI
Untargeted metabolomic analyses reveal the diversity and plasticity of the specialized metabolome in seeds of different Camelina sativa varieties
S. Boutet,L. Barreda,François Perreau,Grégory Mouille,Etienne Delannoy,Etienne Delannoy,Marie-Laure Martin-Magniette,Marie-Laure Martin-Magniette,A. Monti,Loïc Lepiniec,F. Zanetti,Massimiliano Corso +11 more
TL;DR: In this article, a comprehensive annotation of camelina seed SMs combining molecular and correlation network analyses, which cluster SMs based on their chemical structures and co-accumulation patterns, respectively, was achieved.
Journal ArticleDOI
Preference and performance of Lepidoptera varies with tree age in juniper woodlands
TL;DR: As trees age, they undergo significant physiological and morphological changes, but tree ontogeny and its impacts on herbivores are often overlooked as determinants of plant–herbivore population dynamics and the strength of plant-herBivore interactions.
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Combination of network and molecule structure accurately predicts competitive inhibitory interactions
Zahra Razaghi-Moghadam,Zahra Razaghi-Moghadam,Ewelina M. Sokolowska,Marcin A. Sowa,Aleksandra Skirycz,Aleksandra Skirycz,Zoran Nikoloski,Zoran Nikoloski +7 more
TL;DR: In this paper, a supervised machine learning approach, called CIRI, is proposed to determine whether or not a metabolite is involved in a competitive inhibitory regulatory interaction with an enzyme.
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