Elementary and global aspects of calcium signalling.
TLDR
Using Ca2+ imaging techniques, the opening of individual channels has now been visualized and models have been proposed to explain how these elementary events are coordinated to generate the global Ca 2+ signals that regulate cellular activity.Abstract:
Calcium is a ubiquitous second messenger used to regulate a wide range of cellular processes. This role in signalling has to be conducted against the rigid homeostatic mechanisms that ensure that the resting level of Ca2+ is kept low (i.e. between 20 and 100 nmol l-1) in order to avoid the cytotoxic effects of a prolonged elevation of [Ca2+]. Cells have evolved a sophisticated signalling system based on the generation of brief pulses of Ca2+ which enables this ion to be used as a messenger, thus avoiding its toxic effects. Such Ca2+ spikes usually result from the coordinated release of Ca2+ from internal stores using either inositol 1,4,5-trisphosphate or ryanodine receptors. Using Ca2+ imaging techniques, the opening of individual channels has now been visualized and models have been proposed to explain how these elementary events are coordinated to generate the global Ca2+ signals that regulate cellular activity.read more
Citations
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Mechanotransduction in the Lung Pressure-induced endothelial Ca 2 oscillations in lung capillaries
TL;DR: Kuebler et al. as mentioned in this paper determined endothelial cytosolic Ca2 concentration in lung venular capillaries by the fura 2 ratioing method and found that pressure elevation increased mean endothelial [Ca2 ]i by Ca2 influx through gadolinium-inhibitable channels and amplified [Ca 2 ]i oscillations by Ca 2 release from intracellular stores.
Journal ArticleDOI
The role of calcium in activity-dependent neuronal gene regulation.
TL;DR: Recent studies of the signaling from the synapse to the nucleus now allow us to consider how various patterns of synaptic activity could couple with activation of specific nuclear transcription factors and thus regulate neuronal gene expression.
Journal ArticleDOI
Novel mechanisms involved in superoxide anion radical-triggered Ca2+ release from cardiac sarcoplasmic reticulum linked to cyclic ADP-ribose stimulation.
TL;DR: The results presented here provide the first evidence of a messenger role for O2.- radical in cADPR-mediated Ca2+ mobilization in myocardium.
Journal ArticleDOI
Optically Switchable Chelates : Optical Control and Sensing of Metal Ions
TL;DR: Fluorescence imaging of the MC state of nitroBIPS-8-TriA was used to quantify the rate and efficiency of optical switching and to provide a real-time readout of the state of the optically switchable chelate within living cells.
BookDOI
Calcium signaling protocols
TL;DR: Continuous Fluorescent Monitoring of Cellular Calcium Fluxes: A Novel Perfusion System for the Investigation of Inosito(1,4,5)trisphosphate-Dependent Quantal Calcium Release Using Immobilized, Electropermeabilized Cells, Robert A. Wilcox and James Strupish.
References
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Journal ArticleDOI
Inositol trisphosphate and calcium signalling
TL;DR: Inositol trisphosphate is a second messenger that controls many cellular processes by generating internal calcium signals through receptors whose molecular and physiological properties closely resemble the calcium-mobilizing ryanodine receptors of muscle.
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Inositol trisphosphate, a novel second messenger in cellular signal transduction.
TL;DR: Diacylglycerol operates within the plane of the membrane to activate protein kinase C, whereas inositol trisphosphate is released into the cytoplasm to function as a second messenger for mobilizing intracellular calcium.
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A model for receptor-regulated calcium entry
TL;DR: A capacitative model is proposed for the mechanism by which activation of surface membrane receptors causes sustained Ca2+ entry into cells from the extracellular space, which allows forCa2+ release and Ca2-mobilization to be controlled by a single messenger, inositol (1,4,5) trisphosphate.
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Release of Ca2+ from a nonmitochondrial intracellular store in pancreatic acinar cells by inositol-1,4,5-trisphosphate.
TL;DR: It is reported here that micromolar concentrations of Ins1,4,5P3 release Ca2+ from a nonmitochondrial intracellular Ca2- store in pancreatic acinar cells, and the results strongly suggest that this is the same Ca1+ store that is released by acetylcholine.
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Calcium sparks: elementary events underlying excitation-contraction coupling in heart muscle
TL;DR: The calcium spark is the consequence of elementary events underlying excitation-contraction coupling and provides an explanation for both spontaneous and triggered changes in the intracellular calcium concentration in the mammalian heart.