Elementary and global aspects of calcium signalling.
TLDR
Using Ca2+ imaging techniques, the opening of individual channels has now been visualized and models have been proposed to explain how these elementary events are coordinated to generate the global Ca 2+ signals that regulate cellular activity.Abstract:
Calcium is a ubiquitous second messenger used to regulate a wide range of cellular processes. This role in signalling has to be conducted against the rigid homeostatic mechanisms that ensure that the resting level of Ca2+ is kept low (i.e. between 20 and 100 nmol l-1) in order to avoid the cytotoxic effects of a prolonged elevation of [Ca2+]. Cells have evolved a sophisticated signalling system based on the generation of brief pulses of Ca2+ which enables this ion to be used as a messenger, thus avoiding its toxic effects. Such Ca2+ spikes usually result from the coordinated release of Ca2+ from internal stores using either inositol 1,4,5-trisphosphate or ryanodine receptors. Using Ca2+ imaging techniques, the opening of individual channels has now been visualized and models have been proposed to explain how these elementary events are coordinated to generate the global Ca2+ signals that regulate cellular activity.read more
Citations
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Journal ArticleDOI
Expression and characterization of LTx2, a neurotoxin from Lasiodora sp. effecting on calcium channels.
Alexandre A. A. Dutra,Lucas Oliveira Sousa,Rodrigo R. Resende,Rogélio Lopes Brandão,Evanguedes Kalapothakis,Ieso de Miranda Castro +5 more
TL;DR: Ca2+ imaging analysis revealed that the recombinant LTx2 acts on calcium channels of BC3H1 cells, blocking L-type calcium channels.
Journal ArticleDOI
Effect of 17β-Estradiol on Intracellular Ca2+ Levels in Renal Tubular Cells
Wei Chuan Chen,Jin Shiung Cheng,Kang Ju Chou,Kwong Yui Tang,Jong Khing Huang,Li Ling Tseng,Jue Long Wang,Kam Chung Lee,Chung Ren Jan +8 more
TL;DR: Collectively, this study shows that 17β-estradiol evoked a significant internal Ca 2+ release and external Ca2+ entry possibly in a nongenomic manner in Madin Darby canine kidney cells.
Journal ArticleDOI
Distinct modes of molecular regulation of CCL3 induced calcium flux in monocytic cells.
TL;DR: It is shown that CCR5 signalling is dependent on thapsigargin-sensitive Ca2+ stores and on activation of ryanodine receptors as well as InsP3 receptors or store-operated channels and the loss of response in lovastatin treated cells can be rescued by MCD addition, which shows that the cholesterol content in the membrane is only one factor in determining the amount of receptor response.
Book ChapterDOI
Intracellular calcium signaling
TL;DR: This chapter describes the currently known mechanisms responsible for the generation of Ca2+ signals and examples of how Ca2- signals can be shaped in time and space, which appear to be regulated by a growing number of messengers.
Journal ArticleDOI
Dual effect of the antianginal drug fendiline on bladder female transitional carcinoma cells: mobilization of intracellular CA2+ and induction of cell death.
TL;DR: Fendiline exerts a dual effect: mobilization of intracellular Ca2+ and induction of cell death in BFTC cells, and may be partly mediated by inositol 1,4,5-trisphosphate.
References
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Journal ArticleDOI
Inositol trisphosphate and calcium signalling
TL;DR: Inositol trisphosphate is a second messenger that controls many cellular processes by generating internal calcium signals through receptors whose molecular and physiological properties closely resemble the calcium-mobilizing ryanodine receptors of muscle.
Journal ArticleDOI
Inositol trisphosphate, a novel second messenger in cellular signal transduction.
TL;DR: Diacylglycerol operates within the plane of the membrane to activate protein kinase C, whereas inositol trisphosphate is released into the cytoplasm to function as a second messenger for mobilizing intracellular calcium.
Journal ArticleDOI
A model for receptor-regulated calcium entry
TL;DR: A capacitative model is proposed for the mechanism by which activation of surface membrane receptors causes sustained Ca2+ entry into cells from the extracellular space, which allows forCa2+ release and Ca2-mobilization to be controlled by a single messenger, inositol (1,4,5) trisphosphate.
Journal ArticleDOI
Release of Ca2+ from a nonmitochondrial intracellular store in pancreatic acinar cells by inositol-1,4,5-trisphosphate.
TL;DR: It is reported here that micromolar concentrations of Ins1,4,5P3 release Ca2+ from a nonmitochondrial intracellular Ca2- store in pancreatic acinar cells, and the results strongly suggest that this is the same Ca1+ store that is released by acetylcholine.
Journal ArticleDOI
Calcium sparks: elementary events underlying excitation-contraction coupling in heart muscle
TL;DR: The calcium spark is the consequence of elementary events underlying excitation-contraction coupling and provides an explanation for both spontaneous and triggered changes in the intracellular calcium concentration in the mammalian heart.