Elementary and global aspects of calcium signalling.
TLDR
Using Ca2+ imaging techniques, the opening of individual channels has now been visualized and models have been proposed to explain how these elementary events are coordinated to generate the global Ca 2+ signals that regulate cellular activity.Abstract:
Calcium is a ubiquitous second messenger used to regulate a wide range of cellular processes. This role in signalling has to be conducted against the rigid homeostatic mechanisms that ensure that the resting level of Ca2+ is kept low (i.e. between 20 and 100 nmol l-1) in order to avoid the cytotoxic effects of a prolonged elevation of [Ca2+]. Cells have evolved a sophisticated signalling system based on the generation of brief pulses of Ca2+ which enables this ion to be used as a messenger, thus avoiding its toxic effects. Such Ca2+ spikes usually result from the coordinated release of Ca2+ from internal stores using either inositol 1,4,5-trisphosphate or ryanodine receptors. Using Ca2+ imaging techniques, the opening of individual channels has now been visualized and models have been proposed to explain how these elementary events are coordinated to generate the global Ca2+ signals that regulate cellular activity.read more
Citations
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Differential regulation of Ca(2+) sparks and Ca(2+) waves by UTP in rat cerebral artery smooth muscle cells.
TL;DR: The concept that vasoconstrictors shift Ca( 2+) signaling modalities from Ca(2+) sparks to Ca (2+) waves through the concerted actions of PKC on the Ca(1+) sensitivity of RyR channels, and of inositol trisphosphate on IP(3) receptors to generate Ca(Two+) waves, is supported.
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Coupling of Ca(2+) to CREB activation and gene expression in intact cerebral arteries from mouse : roles of ryanodine receptors and voltage-dependent Ca(2+) channels.
TL;DR: The data suggest that membrane depolarization increases [Ca(2+)](i) through activation of VDCCs, leading to increased P-CREB and c-fos levels, and that RyRs have a profound effect on this pathway by indirectly regulating Ca(2+) entry through VD CCs.
Journal ArticleDOI
Calcium puffs are generic InsP3-activated elementary calcium signals and are downregulated by prolonged hormonal stimulation to inhibit cellular calcium responses
Stephen C. Tovey,Patrick De Smet,Peter Lipp,David D. Thomas,Kenneth W. Young,Ludwig Missiaen,Humbert De Smedt,Jan B. Parys,Michael J. Berridge,Jan Willem Thuring,Andrew B. Holmes,Martin D. Bootman,Martin D. Bootman +12 more
TL;DR: Hormonal stimulation of SH-SY5Y neuroblastoma cells and HeLa cells for several hours downregulated inositol 1,4,5-trisphosphate expression and concomitantly altered the properties of the Ca(2+) puffs, which showed a lower propensity to trigger regenerative Ca( 2+) waves.
Journal ArticleDOI
Intercellular Ca2+ wave propagation through gap-junctional Ca2+ diffusion: a theoretical study.
TL;DR: The concentration of diffusive cytoplasmic calcium buffers and the maximal rate of CICR, in the case of inositol 1,4,5-trisphosphate (IP3) receptor calcium release channels set by the IP(3) concentration, are shown to be further determinants of wave behavior.
Journal ArticleDOI
Calcium-regulated DNA binding and oligomerization of the neuronal calcium-sensing protein, calsenilin/DREAM/KChIP3.
Masanori Osawa,Kit I. Tong,Christina Lilliehook,Wilma Wasco,Joseph D. Buxbaum,H.-Y. Mary Cheng,Josef M. Penninger,Mitsuhiko Ikura,James B. Ames +8 more
TL;DR: Physicochemical characterization of calcium binding, oligomerization, and DNA binding of human calsenilin/DREAM/KChIP3, a member of the recoverin branch of the EF-hand superfamily, interacts with presenilins, serves as a calcium-regulated transcriptional repressor, and interacts with A-type potassium channels.
References
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Journal ArticleDOI
Inositol trisphosphate and calcium signalling
TL;DR: Inositol trisphosphate is a second messenger that controls many cellular processes by generating internal calcium signals through receptors whose molecular and physiological properties closely resemble the calcium-mobilizing ryanodine receptors of muscle.
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TL;DR: Diacylglycerol operates within the plane of the membrane to activate protein kinase C, whereas inositol trisphosphate is released into the cytoplasm to function as a second messenger for mobilizing intracellular calcium.
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A model for receptor-regulated calcium entry
TL;DR: A capacitative model is proposed for the mechanism by which activation of surface membrane receptors causes sustained Ca2+ entry into cells from the extracellular space, which allows forCa2+ release and Ca2-mobilization to be controlled by a single messenger, inositol (1,4,5) trisphosphate.
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Release of Ca2+ from a nonmitochondrial intracellular store in pancreatic acinar cells by inositol-1,4,5-trisphosphate.
TL;DR: It is reported here that micromolar concentrations of Ins1,4,5P3 release Ca2+ from a nonmitochondrial intracellular Ca2- store in pancreatic acinar cells, and the results strongly suggest that this is the same Ca1+ store that is released by acetylcholine.
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Calcium sparks: elementary events underlying excitation-contraction coupling in heart muscle
TL;DR: The calcium spark is the consequence of elementary events underlying excitation-contraction coupling and provides an explanation for both spontaneous and triggered changes in the intracellular calcium concentration in the mammalian heart.