Elicitation of Potent Neutralizing Antibody Responses by Designed Protein Nanoparticle Vaccines for SARS-CoV-2.
Alexandra C. Walls,Brooke Fiala,Alexandra Schäfer,Samuel Wrenn,Minh N. Pham,Michael E. P. Murphy,Longping V. Tse,Laila Shehata,Megan A. O'Connor,Chengbo Chen,Mary Jane Navarro,Marcos C. Miranda,Deleah Pettie,Rashmi Ravichandran,John C. Kraft,Cassandra Ogohara,Anne L. Palser,Sara Chalk,E-Chiang Lee,Kathryn A. Guerriero,Elizabeth Kepl,Cameron M. Chow,Claire Sydeman,Edgar A. Hodge,Brieann Brown,James T. Fuller,Kenneth H. Dinnon,Lisa E. Gralinski,Sarah R. Leist,Kendra Gully,Thomas B. Lewis,Miklos Guttman,Helen Y. Chu,Kelly K. Lee,Deborah H. Fuller,Ralph S. Baric,Paul Kellam,Lauren Carter,Marion Pepper,Timothy P. Sheahan,David Veesler,Neil P. King +41 more
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TLDR
The structure-based design of self-assembling protein nanoparticle immunogens that elicit potent and protective antibody responses against SARS-CoV-2 in mice are described and the high yield and stability of the assembled nanoparticles suggest that manufacture of the nanoparticle vaccines will be highly scalable.About:
This article is published in Cell.The article was published on 2020-11-25 and is currently open access. It has received 361 citations till now. The article focuses on the topics: Neutralizing antibody.read more
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Book ChapterDOI
Accelerating therapeutic protein design.
TL;DR: In this paper , the main challenges facing therapeutic proteins discovery and development, and how present and future advancements of protein design can accelerate the protein drug pipelines are discussed. But, the authors do not discuss the role of protein structures in protein drug development.
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Intranasal SARS-CoV-2 RBD decorated nanoparticle vaccine enhances viral clearance in the Syrian hamster model.
D.R. Patel,Allen M. Minns,Derek G Sims,Cassandra J. Field,Abigail E Kerr,Talia A. Heinly,Erin Luley,Randall M. Rossi,Carol M. Bator,I. M. Mostafa,Susan Hafenstein,Scott E. Lindner,Troy C. Sutton +12 more
TL;DR: In this article , an intranasal nanoparticle vaccine against SARS-CoV-2 was developed using the SpyCatcher-SpyTag system and grafted to a 60-subunit I3-01 protein scaffold.
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Process Development of a SARS-CoV-2 Nanoparticle Vaccine
Diandra Martinez-Cano,Rashmi Ravichandran,Huong Le,H. Edward Wong,Bharat Jagannathan,Erik J. Liu,William Bailey,Jane Yang,Kelli Matthies,Hedieh Barkhordarian,Bhavana Shah,N. Srinivasan,Jun Zhang,Angel Hsu,Jette Wypych,Jennitte Stevens,Deirdre Murphy Piedmonte,Les P. Miranda,Lauren Carter,Michael E. P. Murphy,Neil P. King,Neil Soice +21 more
TL;DR: In this paper , the authors describe the rapid development of a robust and reproducible process for a two-component self-assembling protein nanoparticle vaccine for SARS-CoV-2.
Journal ArticleDOI
A new strategy: identification of specific antibodies for neutralizing epitope on SARS-CoV-2 S protein by LC-MS/MS combined with immune repertoire
Meng Yu,Zhu Zhu,Yanqun Wang,Pingzhang Wang,Xiaodong Jia,Jie Wang,Lei Liu,Wanbing Liu,Yaqiong Zheng,Guomei Kou,Weiyan Xu,Jing Huang,Fengmin Lu,Xiajuan Zou,Shangen Zheng,Yinying Lu,Jincun Zhao,Hui Dai,Xiaoyan Qin +18 more
TL;DR: In this article , the peptide sequences of the antigen-binding regions (variable region) of neutralizing antibodies were analyzed by protein mass spectrometry, and then, they were mapped to the full BCR repertoire and found the full variable region sequence of neutralising antibodies.
References
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TL;DR: Details are given about protein identification and analysis software that is available through the ExPASy World Wide Web server and the extensive annotation available in the Swiss-Prot database is used.
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