Elicitation of Potent Neutralizing Antibody Responses by Designed Protein Nanoparticle Vaccines for SARS-CoV-2.
Alexandra C. Walls,Brooke Fiala,Alexandra Schäfer,Samuel Wrenn,Minh N. Pham,Michael E. P. Murphy,Longping V. Tse,Laila Shehata,Megan A. O'Connor,Chengbo Chen,Mary Jane Navarro,Marcos C. Miranda,Deleah Pettie,Rashmi Ravichandran,John C. Kraft,Cassandra Ogohara,Anne L. Palser,Sara Chalk,E-Chiang Lee,Kathryn A. Guerriero,Elizabeth Kepl,Cameron M. Chow,Claire Sydeman,Edgar A. Hodge,Brieann Brown,James T. Fuller,Kenneth H. Dinnon,Lisa E. Gralinski,Sarah R. Leist,Kendra Gully,Thomas B. Lewis,Miklos Guttman,Helen Y. Chu,Kelly K. Lee,Deborah H. Fuller,Ralph S. Baric,Paul Kellam,Lauren Carter,Marion Pepper,Timothy P. Sheahan,David Veesler,Neil P. King +41 more
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TLDR
The structure-based design of self-assembling protein nanoparticle immunogens that elicit potent and protective antibody responses against SARS-CoV-2 in mice are described and the high yield and stability of the assembled nanoparticles suggest that manufacture of the nanoparticle vaccines will be highly scalable.About:
This article is published in Cell.The article was published on 2020-11-25 and is currently open access. It has received 361 citations till now. The article focuses on the topics: Neutralizing antibody.read more
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Two-component spike nanoparticle vaccine protects macaques from SARS-CoV-2 infection
Philip J. M. Brouwer,Mitch Brinkkemper,Pauline Maisonnasse,Nathalie Dereuddre-Bosquet,Marloes Grobben,Mathieu Claireaux,Marlon de Gast,Romain Marlin,Virginie Chesnais,Ségolène Diry,Joel D. Allen,Yasunori Watanabe,Yasunori Watanabe,Julia M. Giezen,Gius Kerster,Hannah L. Turner,Karlijn van der Straten,Cynthia A. van der Linden,Yoann Aldon,Thibaut Naninck,Ilja Bontjer,Judith A. Burger,Meliawati Poniman,Anna Z Mykytyn,Nisreen M.A. Okba,Edith E. Schermer,Mariëlle J. van Breemen,Rashmi Ravichandran,Tom G. Caniels,Jelle van Schooten,Nidhal Kahlaoui,Vanessa Contreras,Julien Lemaitre,Catherine Chapon,Raphael Ho Tsong Fang,Julien Villaudy,Kwinten Sliepen,Yme U. van der Velden,Bart L. Haagmans,Godelieve J. de Bree,Eric Ginoux,Andrew B. Ward,Max Crispin,Neil P. King,Sylvie van der Werf,Marit J. van Gils,Roger Le Grand,Rogier W. Sanders +47 more
TL;DR: A two-component protein-based nanoparticle vaccine that displays multiple copies of the SARS-CoV-2 spike protein induces potent neutralizing antibody responses in mice, rabbits and cynomolgus macaques, resulting in strongly reduced viral infection and replication in upper and lower airways.
Posted ContentDOI
Interferon-armed RBD dimer enhances the immunogenicity of RBD for sterilizing immunity against SARS-CoV-2
Shiyu Sun,Yueqi Cai,Tian-Zhang Song,Yang Pu,Lin Cheng,Hairong Xu,Chaoyang Meng,Yifan Lin,Jin Sun,Silin Zhang,Yu Gao,Jian-Bao Han,Xiao-Li Feng,Dan-Dan Yu,Yalan Zhu,Pu Gao,Haidong Tang,Jincun Zhao,Jiaming Yang,Zenxiang Hu,Zheng Zhang,Yang Xin Fu,Yong-Tang Zheng,Hua Peng +23 more
TL;DR: In this article, an interferon-armed RBD dimerized by immunoglobulin fragment (I-R-F) was developed to enhance antigen processing and cross-presentation in draining lymph nodes (DLNs).
Journal ArticleDOI
Profile of SARS-CoV-2.
Franz X. Heinz,Karin Stiasny +1 more
TL;DR: Some of the most important characteristics of this new coronavirus (including gaps in understanding) are described and a perspective of ongoing activities for developing virus-specific countermeasures, such as vaccines and antiviral drugs are provided.
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One dose of COVID-19 nanoparticle vaccine REVC-128 provides protection against SARS-CoV-2 challenge at two weeks post immunization
Maggie Gu,Jonathan L. Torres,Jack Greenhouse,Shannon Wallace,Chi-I Chiang,Abigail M. Jackson,Maciel Porto,Swagata Kar,Yuxing Li,Andrew B. Ward,Yimeng Wang +10 more
TL;DR: In this paper, the authors demonstrate the development of a nanoparticle vaccine candidate, REVC-128, in which multiple trimeric spike ectodomain subunits with glycine (G) at position 614 were multimerized onto nanoparticles, and demonstrate that a single dose of this vaccine induces potent serum neutralizing antibody titer at two weeks post immunization.
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Fast and versatile sequence-independent protein docking for nanomaterials design using RPXDock
TL;DR: RPXDock as discussed by the authors is a fast, flexible, and modular software package for sequence-independent rigid-body protein docking across a wide range of symmetric architectures that is easily customizable for further development.
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Enzymatic assembly of DNA molecules up to several hundred kilobases
Daniel G. Gibson,Lei Young,Ray-Yuan Chuang,J. Craig Venter,Clyde A. Hutchison,Hamilton O. Smith +5 more
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Protein identification and analysis tools in the ExPASy server
Marc R. Wilkins,Elisabeth Gasteiger,Amos Marc Bairoch,Jean Emmanuel Sanchez,Keith L. Williams,Ron D. Appel,Denis Hochstrasser +6 more
TL;DR: Details are given about protein identification and analysis software that is available through the ExPASy World Wide Web server and the extensive annotation available in the Swiss-Prot database is used.
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