Journal ArticleDOI
Ex Vivo Gene Transfer of Brain‐derived Neurotrophic Factor to the Intact Rat Forebrain: Neurotrophic Effects on Cholinergic Neurons
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It is concluded that these cholinergic cell groups are responsive to a low‐level supply (nanograms per day) of BDNF in vivo when the neurotrophin is administered locally in the vicinity of the cell bodies.Abstract:
Neurotrophic effects of human brain-derived neurotrophic factor (hBDNF) on forebrain cholinergic neurons were addressed after ex vivo gene transfer to the intact adult rat brain, using a conditionally immortalized neural progenitor cell line (CINP) engineered to secrete the neurotrophin (2.8 ng/h/10(6) cells). This cell line was derived by repeated retroviral infection of the parental neural precursor line HiB5 followed by subcloning. The cells survived well in the host brain for long periods of time (up to 4 weeks), and induced a hypertrophic response of cholinergic neurons (positive for acetylcholinesterase, choline acetyltransferase or low-affinity nerve growth factor receptor) in the nucleus basalis magnocellularis and striatum. We conclude that these cholinergic cell groups are responsive to a low-level supply (nanograms per day) of BDNF in vivo when the neurotrophin is administered locally in the vicinity of the cell bodies.read more
Citations
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Brain-derived neurotrophic factor in the control human brain, and in Alzheimer's disease and Parkinson's disease.
TL;DR: Recent work on the molecular and cellular biology of BDNF is summarized, including current ideas about its intracellular trafficking, regulated synthesis and release, and actions at the synaptic level, which have considerably expanded the conception ofBDNF actions in the central nervous system.
Journal ArticleDOI
The role of neuronal growth factors in neurodegenerative disorders of the human brain
Bronwen Connor,Mike Dragunow +1 more
TL;DR: The role members of the nerve growth factor family (NGF, BDNF and NT-3) and trk receptors as well as additional growth factors (GDNF, TGF-alpha and IGF-I) may play in neurodegenerative disorders of the human brain are examined.
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Gene therapy: can neural stem cells deliver?
TL;DR: It is speculated on the ways in which neural stem cells might be exploited as delivery vehicles for gene therapy in the CNS.
Journal ArticleDOI
Cell-mediated drug delivery
TL;DR: How immunocytes laden with drugs can cross the blood–brain or blood–tumor barriers to facilitate treatments for infectious diseases, injury, cancer, or inflammatory diseases is reviewed.
Journal ArticleDOI
Establishment and Properties of a Growth Factor-Dependent, Perpetual Neural Stem Cell Line from the Human CNS
TL;DR: V-myc seems to be the most effective gene and a strict requirement for the presence of mitogens in the growth medium is identified, in effect constituting a conditional perpetuality or immortalization of the human neural stem cell line HNSC.100.
References
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TL;DR: Long-term potentiation could still be elicited in slices previously potentiated by exposure to the neurotrophic factors, which implies that these two forms of plasticity may use at least partially independent cellular mechanisms.
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Isolation, Characterization, and use of Stem Cells from the CNS
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Journal ArticleDOI
trkB encodes a functional receptor for brain-derived neurotrophic factor and neurotrophin-3 but not nerve growth factor
Stephen P. Squinto,Trevor Stitt,Aldrich Thomas H,Samuel Davis,Stella M. Blanco,Czeslaw Radziejewski,David J. Glass,Piotr Masiakowski,Mark E. Furth,David M. Valenzuela,Peter S. DiStefano,George D. Yancopoulos +11 more
TL;DR: It is demonstrated that all three of these neuronal survival molecules bind similarly to the low affinity NGF receptor, but that BDNF and NT-3, unlike NGF, do not act via the high affinity N GF receptor.
Journal ArticleDOI
Neurotrophic factors: from molecule to man.
TL;DR: The biology of the recently discovered NGF-related family of neurotrophic factors and ciliary neurotrophic factor and their receptors are reviewed, especially in the context of the therapeutic potential of these factors in the treatment of neurological disorders of the CNS.