Expression cloning of a human GT3 synthase. GD3 AND GT3 are synthesized by a single enzyme.
Jun Nakayama,Michiko N. Fukuda,Yoshio Hirabayashi,Akiko Kanamori,Katsutoshi Sasaki,Tatsunari Nishi,Minoru Fukuda +6 more
TLDR
Results indicate that C seriespolysialogangliosides are synthesized by a ganglioside-specific polysialyltransferase, GD3/GT3ST, that is specifically expressed in neural tissues.About:
This article is published in Journal of Biological Chemistry.The article was published on 1996-02-16 and is currently open access. It has received 95 citations till now. The article focuses on the topics: Expression cloning & Complementary DNA.read more
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The human sialyltransferase family
Anne Harduin-Lepers,Verónica Vallejo-Ruiz,Marie-Ange Krzewinski-Recchi,Bénédicte Samyn-Petit,Sylvain Julien,Philippe Delannoy +5 more
TL;DR: An overview of the 15 different human sialyltransferase cDNAs, the regulation of their occurrence and their involvement in several physiological and pathological processes is presented.
Journal ArticleDOI
Natural antibiotic function of a human gastric mucin against Helicobacter pylori infection.
Masatomo Kawakubo,Yuki Ito,Yukie Okimura,Motohiro Kobayashi,Motohiro Kobayashi,Kyoko Sakura,Susumu Kasama,Michiko N. Fukuda,Minoru Fukuda,Tsutomu Katsuyama,Jun Nakayama +10 more
TL;DR: The unique O-glycans in gastric mucin appeared to function as a natural antibiotic, protecting the host from H. pylori infection.
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Combinatorial ganglioside biosynthesis.
TL;DR: The vital role of glycosphingolipids has been demonstrated by the fact that their complete deficiency in mice leads to early embryonic lethality, and the large number of theoretically possible glycolipid structures that can be formed by combination of different carbohydrate building blocks, linkage positions, and stereochemistry is realized.
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Molecular Cloning and Functional Analysis of Sialyltransferases
TL;DR: It is found that transfection of the GD3 synthase, an alpha 2,8-ST (ST8Sia I), gene triggers cholinergic neuritogenesis in Neuro2a cells through the de novo expression of GD3, suggesting that the GD 3 synthase gene behaves as a neural differentiation inducer.
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Polysialyltransferases: major players in polysialic acid synthesis on the neural cell adhesion molecule
Kiyohiko Angata,Minoru Fukuda +1 more
TL;DR: The presence of polysialic acid is always associated with expression of ST8Sia II and/or IV, suggesting that ST8 sialic Acid II and IV are the key enzymes that control the expression ofpolysIALic acid.
References
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DNA sequencing with chain-terminating inhibitors
TL;DR: A new method for determining nucleotide sequences in DNA is described, which makes use of the 2',3'-dideoxy and arabinon nucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase.
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A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity
TL;DR: A technique for conveniently radiolabeling DNA restriction endonuclease fragments to high specific activity is described, and these "oligolabeled" DNA fragments serve as efficient probes in filter hybridization experiments.
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity
TL;DR: In this article, a technique for conveniently radiolabeling DNA restriction endonuclease fragments to high specific activity is described, where DNA fragments are purified from agarose gels directly by ethanol precipitation and are then denatured and labeled with the large fragment of DNA polymerase I, using random oligonucleotides as primers.
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Selective extraction of polyoma DNA from infected mouse cell cultures.
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Structural features in eukaryotic mRNAs that modulate the initiation of translation.
TL;DR: In higher eukaryotes, translation is modulated at the level of initiation by five aspects of mRNA structure: (i) the m7G cap; (ii) the primary sequence or context surrounding the AUG codon; (iii) the position of the Aug codon, i.e. whether or not it s “first”; (iv) secondary structure both upstream and downstream from the AUU; and (v) leader length.