Journal ArticleDOI
Expression profiling of circulating miRNAs in mouse serum in response to Echinococcus multilocularis infection.
Xiaola Guo,Yadong Zheng +1 more
TLDR
Comparing the host-circulating miRNA expression in the sera from the E. multilocularis-infected and uninfected mice will be helpful to understand the roles of miRNAs in host–parasite interaction and to potentiate serum mi RNAs as diagnostic targets for echinococcosis.Abstract:
Echinococcus multilocularis is a most pathogenic zoonotic tapeworm that causes devastating echinococcosis in both humans and animals. Circulating microRNAs (miRNAs) are stably existed in the serum/plasma of mammalian hosts during helminthic infection. In this study, we compared the host-circulating miRNA expression in the sera from the E. multilocularis-infected and uninfected mice. A total of 58 host-origin serum miRNAs were differentially expressed (2 ⩾ fold change, P < 0·05), of which 21 were upregulated and 37 were significantly downregulated. Consistent with the sequencing data, quantitative polymerase chain reaction (PCR) results showed that the expression levels of four miRNAs were elevated gradually and one decreased gradually at the E. multilocularis infection time points. Moreover, seven of E. multilocularis specific miRNAs were identified in the sera. Real-time PCR analyses further demonstrated that only two parasite-derived miRNAs (emu-miR-10 and emu-miR-227) were specifically amplified in all the sera from mice infected with E. multilocularis. These findings will be helpful to understand the roles of miRNAs in host-parasite interaction and to potentiate serum miRNAs as diagnostic targets for echinococcosis.read more
Citations
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Journal ArticleDOI
Parasite-derived microRNAs in plasma as novel promising biomarkers for the early detection of hydatid cyst infection and post-surgery follow-up.
Zahra Alizadeh,Mahmoud Mahami-Oskouei,Adel Spotin,Tohid Kazemi,Ehsan Ahmadpour,Pengfei Cai,Dariush Shanehbandi,Najibeh Shekari +7 more
TL;DR: It seems that these parasite-derived miRNAs, especially egr-miR-71, could serve as novel promising biomarkers for the early diagnosis and monitoring of hydatidosis.
Journal ArticleDOI
Comprehensive Analysis of Non-coding RNA Profiles of Exosome-Like Vesicles From the Protoscoleces and Hydatid Cyst Fluid of Echinococcus granulosus.
TL;DR: For the first time, the ncRNAs profiles in PSC–ELVs and HF-ELVs that might be involved in host immunity and pathogenesis are identified, and the n cRNAs data of E. granulosus ELVs nc RNAs in the parasite–host interactions are enriched.
Journal ArticleDOI
Parasite-derived circulating microRNAs as biomarkers for the detection of human Schistosoma japonicum infection.
TL;DR: RT-PCR analysis showed that two parasite-derived miRNAs could detect infected individuals with low infection intensity with moderate sensitivity/specificity values of 66%/68% and 55%/80%, respectively, while the serum level of sja-miR-2c-5p correlated significantly with faecal egg counts, whereas the other five mi RNAs did not.
Journal ArticleDOI
Parasitic Helminth-Derived microRNAs and Extracellular Vesicle Cargos as Biomarkers for Helminthic Infections.
TL;DR: In this article, the authors discuss the status and potential of helminth-derived circulating miRNAs and extracellular vesicles (EVs) as novel diagnostic tools.
Journal ArticleDOI
Circulating MicroRNAs As Potential Biomarkers for Veterinary Infectious Diseases.
Hao Dong,Qiang Gao,Xiaowei Peng,Sun Yu,Han Tao,Bolin Zhao,Y. H. Liu,Chuanbin Wang,Song Xiaohui,Jiajun Wu,Yang Lin +10 more
TL;DR: An update of preclinical studies on using circulating miRNAs as diagnostic biomarkers to combat infectious diseases that affect domestic animals is summarized.
References
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NF-κB-dependent induction of microRNA miR-146, an inhibitor targeted to signaling proteins of innate immune responses
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