Genetic Applications of an Inverse Polymerase Chain Reaction
TLDR
The feasibility of IPCR is shown by amplifying the sequences that flank an IS1 element in the genome of a natural isolate of Escherichia coli.Abstract:
A method is presented for the rapid in vitro amplification of DNA sequences that flank a region of known sequence. The method uses the polymerase chain reaction (PCR), but it has the primers oriented in the reverse direction of the usual orientation. The template for the reverse primers is a restriction fragment that has been ligated upon itself to form a circle. This procedure of inverse PCR (IPCR) has many applications in molecular genetics, for example, the amplification and identification of sequences flanking transposable elements. In this paper we show the feasibility of IPCR by amplifying the sequences that flank an IS1 element in the genome of a natural isolate of Escherichia coli.read more
Citations
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Journal ArticleDOI
Structural basis for the methylation of A1408 in 16S rRNA by a panaminoglycoside resistance methyltransferase NpmA from a clinical isolate and analysis of the NpmA interactions with the 30S ribosomal subunit
Nilofer Husain,Sonja Obranić,Lukasz Koscinski,Jayaraman Seetharaman,Fedora Babić,Janusz M. Bujnicki,Gordana Maravić-Vlahoviček,J. Sivaraman +7 more
TL;DR: Experimental and theoretical analyses suggest that NpmA flips out the target nucleotide A1408 to carry out the methylation of kanamycin, which poses a threat to the successful use of aminoglycosides in clinical practice.
Journal ArticleDOI
Simultaneous amplification of 5′ and 3′ cDNA ends based on template-switching effect and inverse PCR
Junchao Huang,Feng Chen +1 more
TL;DR: A new approach for simultaneously amplifying the 5' and 3' ends of a desired cDNA is described, which is reliable and easy to perform, and very useful for isolating cDNA species of rare transcripts.
Journal ArticleDOI
Albino Leaf 2 is involved in the splicing of chloroplast group I and II introns in rice
Changhong Liu,Haitao Zhu,Yi Xing,Jianjie Tan,Chen Xionghui,Jianjun Zhang,Haifeng Peng,Qingjun Xie,Zemin Zhang +8 more
TL;DR: The Albino Leaf 2 (AL2) gene is involved in the splicing of chloroplast group I and II introns and is responsible forchloroplast development in rice.
Patent
Methods of Identification Using Methylation of CPG
TL;DR: In this article, the materials and methods for the identification of methylated nucleotides in samples of genomic DNA were described, and the present invention also relates to methods of diagnosis of specific conditions by identification of specific methylated DNA.
Journal ArticleDOI
Inverse polymerase chain reaction for cloning cellular sequences adjacent to integrated hepatitis B virus DNA in hepatocellular carcinomas
Daw–Jen Tsuei,Pei-Jer Chen,Ming Yang Lai,Ding-Shinn Chen,Czau Siung Yang,Jen Yang Chen,Tsuey Ying Hsu +6 more
TL;DR: By employing the inverse polymerase chain reaction (IPCR) method, two adjacent cellular sequences were cloned from 11 HBV integrants in three HCCs and one of the flanking cellular sequences was identified as the human 28S rRNA gene, the other was not found homologous to any known human sequences.
References
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DNA typing from single hairs
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