Genetic Applications of an Inverse Polymerase Chain Reaction
TLDR
The feasibility of IPCR is shown by amplifying the sequences that flank an IS1 element in the genome of a natural isolate of Escherichia coli.Abstract:
A method is presented for the rapid in vitro amplification of DNA sequences that flank a region of known sequence. The method uses the polymerase chain reaction (PCR), but it has the primers oriented in the reverse direction of the usual orientation. The template for the reverse primers is a restriction fragment that has been ligated upon itself to form a circle. This procedure of inverse PCR (IPCR) has many applications in molecular genetics, for example, the amplification and identification of sequences flanking transposable elements. In this paper we show the feasibility of IPCR by amplifying the sequences that flank an IS1 element in the genome of a natural isolate of Escherichia coli.read more
Citations
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DNA molecular markers in plant breeding: current status and recent advancements in genomic selection and genome editing
Muhammad Azhar Nadeem,Muhammad Amjad Nawaz,Muhammad Qasim Shahid,Yıldız Doğan,Gönül Cömertpay,Mehtap Yildiz,Rüştü Hatipoğlu,Fiaz Ahmad,Ahmad Alsaleh,Nitin M. Labhane,Hakan Özkan,Gyuhwa Chung,Faheem Shehzad Baloch +12 more
TL;DR: The progress made in molecular plant breeding, genetics, genomic selection and genome editing has contributed to a more comprehensive understanding of molecular markers and provided deeper insights into the diversity available for crops and greatly complemented breeding stratagems.
Journal ArticleDOI
The regulatory gene areA mediating nitrogen metabolite repression in Aspergillus nidulans. Mutations affecting specificity of gene activation alter a loop residue of a putative zinc finger.
B Kudla,Mark X. Caddick,Tim Langdon,Nilce Maria Martinez-Rossi,C F Bennett,S Sibley,R W Davies,Herbert N. Arst +7 more
TL;DR: Results show that the regulatory gene areA mediating nitrogen metabolite repression in Aspergillus nidulans reverts to a partly reciprocal phenotype by replacing the mutant valine by methionine, and also identifies sequence changes associated with specificity mutations.
Journal ArticleDOI
A subtilisin-like serine protease involved in the regulation of stomatal density and distribution in Arabidopsis thaliana
Dieter Berger,Thomas Altmann +1 more
TL;DR: It is proposed that SDD1 acts as a processing protease involved in the mediation of a signal that controls the development of cell lineages that lead to guard cell formation in Arabidopsis thaliana.
Patent
Short cycle methods for sequencing polynucleotides
TL;DR: In this article, a polynucleotide sequence is sequenced by stopping an extension cycle in a sequence by synthesis reaction before the reaction has run to near or full completion, which is called stopping the cycle.
Journal ArticleDOI
Type I Repressors of P Element Mobility
Gregory B. Gloor,Christine R. Preston,Dena M. Johnson-Schlitz,Nadine A. Nassif,Randall W. Phillis,Wendy K. Benz,Hugh M. Robertson,William R. Engels +7 more
TL;DR: A family of repressor elements, referred to as type I repressors, were identified by their repressor functions and their lack of any deletion within the first two-thirds of the canonical P sequence as discussed by the authors.
References
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DNA typing from single hairs
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