Genetic Applications of an Inverse Polymerase Chain Reaction
TLDR
The feasibility of IPCR is shown by amplifying the sequences that flank an IS1 element in the genome of a natural isolate of Escherichia coli.Abstract:
A method is presented for the rapid in vitro amplification of DNA sequences that flank a region of known sequence. The method uses the polymerase chain reaction (PCR), but it has the primers oriented in the reverse direction of the usual orientation. The template for the reverse primers is a restriction fragment that has been ligated upon itself to form a circle. This procedure of inverse PCR (IPCR) has many applications in molecular genetics, for example, the amplification and identification of sequences flanking transposable elements. In this paper we show the feasibility of IPCR by amplifying the sequences that flank an IS1 element in the genome of a natural isolate of Escherichia coli.read more
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Identification and association study with lung cancer for novel insertion polymorphisms of human endogenous retrovirus
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Genomic organization and tissue-specific expression of hepcidin in the pacific mutton hamlet, Alphestes immaculatus (Breder, 1936).
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Event-specific qualitative and quantitative PCR detection of roundup ready event GT73 based on the 3′-integration junction
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Characterization of T-DNA insertions in transgenic grapevines obtained by Agrobacterium -mediated transformation
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TL;DR: Inverse PCR was used to identify T-DNA/plant junctions in 49 transgenic grapevines produced via Agrobacterium-mediated transformation as discussed by the authors, where microsimilarities were found associated with the joining points.
References
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