Genetic Applications of an Inverse Polymerase Chain Reaction
TLDR
The feasibility of IPCR is shown by amplifying the sequences that flank an IS1 element in the genome of a natural isolate of Escherichia coli.Abstract:
A method is presented for the rapid in vitro amplification of DNA sequences that flank a region of known sequence. The method uses the polymerase chain reaction (PCR), but it has the primers oriented in the reverse direction of the usual orientation. The template for the reverse primers is a restriction fragment that has been ligated upon itself to form a circle. This procedure of inverse PCR (IPCR) has many applications in molecular genetics, for example, the amplification and identification of sequences flanking transposable elements. In this paper we show the feasibility of IPCR by amplifying the sequences that flank an IS1 element in the genome of a natural isolate of Escherichia coli.read more
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Arabidopsis Vegetative Storage Protein Is an Anti-Insect Acid Phosphatase
Yilin Liu,Ji-Eun Ahn,Sumana Datta,Ron A. Salzman,Jaewoong Moon,Beatrice Huyghues-Despointes,Barry R. Pittendrigh,Larry L. Murdock,Hisashi Koiwa,Keyan Zhu-Salzman +9 more
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The Hermes transposable element from the house fly, Musca domestica, is a short inverted repeat-type element of the hobo, Ac, and Tam3 (hAT) element family
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Diagnostic tool for the identification of MLL rearrangements including unknown partner genes
Claus Meyer,Bjoern Schneider,Martin Reichel,Sieglinde Angermueller,Sabine Strehl,Susanne Schnittger,Claudia Schoch,Mieke W. J. C. Jansen,Jacques J.M. van Dongen,Rob Pieters,Oskar A. Haas,Theo Dingermann,Thomas Klingebiel,Rolf Marschalek +13 more
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Breakpoints of the t(11;18)(q21;q21) in Mucosa-associated Lymphoid Tissue (MALT) Lymphoma Lie within or near the Previously Undescribed Gene MALT1 in Chromosome 18
Jeffrey A. Morgan,Yi Yin,Alexander D. Borowsky,Frank C. Kuo,Nicole Nourmand,Jason I. Koontz,Carol Reynolds,Lee Soreng,Constance A. Griffin,Fiona Graeme-Cook,Nancy L. Harris,Dennis D. Weisenburger,Geraldine S. Pinkus,Jonathan A. Fletcher,Jeffrey Sklar +14 more
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Molecular genetic basis for deficient acetaminophen glucuronidation by cats: UGT1A6 is a pseudogene, and evidence for reduced diversity of expressed hepatic UGT1A isoforms
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References
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