Genetic Applications of an Inverse Polymerase Chain Reaction
TLDR
The feasibility of IPCR is shown by amplifying the sequences that flank an IS1 element in the genome of a natural isolate of Escherichia coli.Abstract:
A method is presented for the rapid in vitro amplification of DNA sequences that flank a region of known sequence. The method uses the polymerase chain reaction (PCR), but it has the primers oriented in the reverse direction of the usual orientation. The template for the reverse primers is a restriction fragment that has been ligated upon itself to form a circle. This procedure of inverse PCR (IPCR) has many applications in molecular genetics, for example, the amplification and identification of sequences flanking transposable elements. In this paper we show the feasibility of IPCR by amplifying the sequences that flank an IS1 element in the genome of a natural isolate of Escherichia coli.read more
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TDNAscan: A Software to Identify Complete and Truncated T-DNA Insertions.
Liang Sun,Yinbing Ge,J. Alan Sparks,Zachary T. Robinson,Xiaofei Cheng,Jiangqi Wen,Elison B. Blancaflor +6 more
TL;DR: This paper presents a next generation sequencing (NGS)-based platform to facilitate the identification of complete and truncated T-DNA insertions and presents a case study in which TDNAscan was used to determine that the recessive Arabidopsis thaliana hypersensitive to latrunculin B (hlb3) mutant isolated in a forward genetic screen of T- DNA mutagenized plants encodes a class II FORMIN.
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Differential expression of the ornithine decarboxylase gene during carposporogenesis in the thallus of the red seaweed Grateloupia imbricata (Halymeniaceae).
Pilar García-Jiménez,Federico García-Maroto,Jose Antonio Garrido-Cardenas,Cristina Ferrándiz,Rafael R. Robaina +4 more
TL;DR: The cloning of the ornithine decarboxylase gene from a red seaweed, Grateloupia imbricata, the characterization of its expression throughout the reproductive process, and how polyamines are involved in seaweed reproduction are demonstrated.
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Efficient expression of the Paramecium calmodulin gene in Escherichia coli after four TAA-to-CAA changes through a series of polymerase chain reactions.
TL;DR: The Paramecium calmodulin gene in Escherichia coli is expressed by changing the four TAA codons in this gene to CAAs by three polymerase chain reactions (PCRs) and cloning the product into the expression vector pKK223-3 immediately downstream of its trp-lac hybrid promoter.
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Molecular Cloning of cDNAs and Genes for Three α-Glucosidases from European Honeybees, Apis mellifera L., and Heterologous Production of Recombinant Enzymes in Pichia pastoris
Mamoru Nishimoto,Haruhide Mori,Tsuneharu Moteki,Yukiko Takamura,Gaku Iwai,Yu Miyaguchi,Masayuki Okuyama,Jintanart Wongchawalit,Rudee Surarit,Jisnuson Svasti,Atsuo Kimura,Seiya Chiba +11 more
TL;DR: High homologies (38–44% identity) were found in the deduced amino acid sequences of HBGases I, II, and III from European honeybees, and the sequences were analyzed.
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Cloning and analysis of the MAT1-2-1 gene from the traditional Chinese medicinal fungus Ophiocordyceps sinensis.
TL;DR: This report describes, for the first time, a mating-type gene of O. sinensis, and shows low sequence similarities with C. militaris and C. takaomontana in both MAT1-2-1 and the DNA lyase gene.
References
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DNA typing from single hairs
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