Journal ArticleDOI
Graphene oxide-chitosan nanocomposite based electrochemical DNA biosensor for detection of typhoid
Anu Singh,Anu Singh,Gaurav Sinsinbar,Meenakshi Choudhary,Veeresh Kumar,Renu Pasricha,H. N. Verma,Surinder P. Singh,Kavita Arora +8 more
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TLDR
The excellent performance of biosensor is attributed to large surface-to-volume ratio and good electrochemical activity of graphene oxide, and good biocompatibility of chitosan, which enhances the DNA immobilization and facilitate electron transfer between DNA and electrode surface (ITO).Abstract:
Graphene oxide (GO)-Chitosan (CHI) nano-composite is employed for the development of DNA based electrochemical biosensor for diagnosis of typhoid. Biosensor has been prepared by covalent immobilization of Salmonella typhi specific 5′-amine labeled single stranded (ss) DNA probe on GO-CHI/ITO via glutaraldehyde. Differential pulse voltammetry (DPV) studies revealed good specificity and ability of ssDNA/GO-CHI/ITO biosensor to distinguish complementary, non-complementary and one base mismatch sequences. The ssDNA/GO-CHI/ITO biosensor showed detection range of 10 fM to 50 nM and LOD 10 fM within 60 s hybridization times for complementary sequence. Further, ssDNA/GO-CHI/ITO bioelectrode is able to detect complementary target present in serum samples with LOD of 100 fM at 25 °C. The excellent performance of biosensor is attributed to large surface-to-volume ratio and good electrochemical activity of graphene oxide, and good biocompatibility of chitosan, which enhances the DNA immobilization and facilitate electron transfer between DNA and electrode surface (ITO).read more
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A review on graphene-based nanocomposites for electrochemical and fluorescent biosensors
TL;DR: Graphene and its oxygenated derivatives, including reduced graphene oxide (rGO), are becoming an important class of nanomaterials in the field of biosensors as discussed by the authors, and the discovery of graphene has spectacularly accelerated research on fabricating low-cost electrode materials because of its unique physical properties, including high specific surface area, high carrier mobility, high electrical conductivity, flexibility.
Journal ArticleDOI
Recent trends in carbon nanomaterial-based electrochemical sensors for biomolecules: A review
TL;DR: This review compares different carbon nanomaterial strategies for creating electrochemical sensors for biomolecules and addresses enzyme-based electrodes that are used to detect non-electroactive species such as glucose, alcohols, and proteins.
Journal ArticleDOI
Recent advances in graphene-based biosensor technology with applications in life sciences
TL;DR: This review will present the research conducted with antibodies, DNA molecules and, enzymes to develop biosensors that use graphene and its derivatives as scaffolds to produce effective biosensor able to detect and identify a variety of diseases, pathogens, and biomolecules linked to diseases.
Journal ArticleDOI
Electrophoretic deposition of chitosan-based composite coatings for biomedical applications: A review
Egemen Avcu,Egemen Avcu,Egemen Avcu,Fatih Erdem Bastan,Fatih Erdem Bastan,Hasan Zuhudi Abdullah,Hasan Zuhudi Abdullah,Muhammad Atiq Ur Rehman,Muhammad Atiq Ur Rehman,Yasemin Yıldıran Avcu,Yasemin Yıldıran Avcu,Aldo R. Boccaccini +11 more
TL;DR: A comprehensive review of the field of electrophoretic deposition of chitosan-based composite coatings by highlighting their microstructural, mechanical, surface, and biological properties is presented in this paper.
Journal ArticleDOI
Recent advances in electrochemical biosensing schemes using graphene and graphene-based nanocomposites
TL;DR: In this article, a comprehensive overview of the field apart from providing intensive information of the fabrication, properties, characterization and EC applications of graphene and its nanocomposites is provided, along with two key challenges, the lack of international regulatory guidelines for nanotoxicity analysis and potential mass production of analytical devices.
References
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