hERG1a and hERG1b potassium channel subunits directly interact and preferentially form heteromeric channels
TLDR
HERG1b preferentially forms heteromeric ion channels with hERG1a at the plasma membrane, and multiple lines of evidence indicated a physical interaction between hERG 1a and hERG2b, consistent with them forming heteromerics channels.About:
This article is published in Journal of Biological Chemistry.The article was published on 2017-12-29 and is currently open access. It has received 19 citations till now. The article focuses on the topics: Ion channel & Potassium channel.read more
Citations
More filters
Journal ArticleDOI
Genetically Encoded Fluorescent Biosensors Illuminate the Spatiotemporal Regulation of Signaling Networks.
TL;DR: In an effort to encapsulate the breadth over which fluorescent biosensors have expanded, this work endeavored to assemble a comprehensive list of published engineered bios Sensors, and discusses many of the molecular designs utilized in their development.
Journal ArticleDOI
Drug-induced Proarrhythmia and Torsade de Pointes: A Primer for Students and Practitioners of Medicine and Pharmacy
J. Rick Turner,Ignacio Rodriguez,Emily H Mantovani,Gary Gintant,Peter R. Kowey,Ralph J Klotzbaugh,Krishna Prasad,Philip T. Sager,Norman Stockbridge,Colette Strnadova +9 more
TL;DR: This primer reviews the clinical implications of a drug's identified proarrhythmic liability, the issues associated with these safety‐related withdrawals, and the actions taken by the International Council for Harmonisation of Technical Requirements for Pharmaceuticals for Human Use (ICH) and by regulatory agencies in terms of changing drug development practices and introducing new nonclinical and clinical tests to asses proarrhalic liability.
Journal ArticleDOI
A microtranslatome coordinately regulates sodium and potassium currents in the human heart.
Catherine A. Eichel,Erick B. Ríos-Pérez,Fang Liu,Margaret B. Jameson,David K. Jones,Jennifer J. Knickelbine,Gail A. Robertson +6 more
TL;DR: It is found that roughly half the hERG translational complexes contain SCN5A transcripts, and association and coordinate regulation of transcripts in discrete ‘microtranslatomes’ represents a new paradigm controlling electrical activity in heart and other excitable tissues.
Journal ArticleDOI
Fentanyl-Induced Block of hERG Channels Is Exacerbated by Hypoxia, Hypokalemia, Alkalosis, and the Presence of hERG1b.
TL;DR: It is demonstrated that heterologously expressed human ether a-go-go–related gene (hERG) 1a/1b channels, which more closely resemble rapidly activating delayed rectifier potassium current in the human heart, are blocked by fentanyl with a 3-fold greater potency than the previously studied hERG1a expressed alone.
Journal ArticleDOI
Long QT Syndrome KCNH2 Variant Induces hERG1a/1b Subunit Imbalance in Patient-Specific Induced Pluripotent Stem Cell-Derived Cardiomyocytes.
Li Feng,Jianhua Zhang,ChangHwan Lee,Gina Kim,Fang Liu,Andrew J. Petersen,Evi Lim,Corey L. Anderson,Kate M. Orland,Gail A. Robertson,Lee L. Eckhardt,Craig T. January,Timothy J. Kamp +12 more
TL;DR: In this paper, patient-specific induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) were derived from a LQT syndrome type 2 (LQT2) patient carrying the PAS domain variant hERG1-H70R.
References
More filters
Journal ArticleDOI
Expression pattern of the ether-a-go-go-related (ERG) family proteins in the adult mouse central nervous system: evidence for coassembly of different subunits.
Leonardo Guasti,Emanuele Cilia,Olivia Crociani,Giovanna Hofmann,Simone Polvani,Andrea Becchetti,Enzo Wanke,Filippo Tempia,Annarosa Arcangeli +8 more
TL;DR: The finding that ERG proteins often have an overlapping expression suggests that neuronal ERG currents could be determined, at least in part, by heterotetrameric ERG channels, as demonstrated by showing that the two isoforms coassemble in mouse brain.
Journal ArticleDOI
Heteromeric assembly of human ether-à-go-go-related gene (hERG) 1a/1b channels occurs cotranslationally via N-terminal interactions.
TL;DR: HERG1 hetero-oligomerization occurs in the endoplasmic reticulum where co-expression of N-terminal fragments with hERG1 subunits disrupted oligomerization and core glycosylation, a cotranslational event.
Journal ArticleDOI
Direct interaction of eag domains and cyclic nucleotide-binding homology domains regulate deactivation gating in hERG channels.
TL;DR: It is found that most channels bearing individual alanine mutations in the S4–S5 linker were directly regulated by recombinant eag domains fused to a cyan fluorescent protein (N-eag-CFP) and had robust Förster resonance energy transfer (FRET).
Journal ArticleDOI
Rescue of Aberrant Gating by a Genetically Encoded PAS (Per-Arnt-Sim) Domain in Several Long QT Syndrome Mutant Human Ether-á-go-go-related Gene Potassium Channels
TL;DR: Results reveal a putative “gating face” in the PAS domain where mutations within this region form functional channels with altered gating properties, and show that NPAS is a general means for rescuing aberrant gating in hERG LQT2 mutant channels and may be a potential biological therapeutic.
Journal ArticleDOI
Dynamic conformational changes of extracellular S5-P linkers in the hERG channel.
Min Jiang,Mei Zhang,Innokenty V. Maslennikov,Jie Liu,Dong-Mei Wu,Yuliya V. Korolkova,Alexander S. Arseniev,Eugene V. Grishin,Gea-Ny Tseng +8 more
TL;DR: It is proposed that the four S5–P linkers in a hERG channel can engage in dynamic conformational changes during channel gating, and interactions between S5-P linker from neighbouring subunits contribute importantly to channel inactivation.