Heterodimeric complexes of Hop2 and Mnd1 function with Dmc1 to promote meiotic homolog juxtaposition and strand assimilation
Yi-Kai Chen,Chih-Hsiang Leng,Heidi Olivares,Ming-Hui Lee,Yuan-Chih Chang,Wen-Mei Kung,Shih-Chieh Ti,Yu-Hui Lo,Andrew H.-J. Wang,Chia-Seng Chang,Douglas K. Bishop,Yi-Ping Hsueh,Ting-Fang Wang +12 more
TLDR
The genetic and biochemical results suggest that Hop2, Mnd1, and Dmc1 are functionally interdependent during meiotic DNA recombination.Abstract:
Saccharomyces cerevisiae Hop2 and Mnd1 are abundant meiosisspecific chromosomal proteins, and mutations in the corresponding genes lead to defects in meiotic recombination and in homologous chromosome interactions during mid-prophase. Analysis of various double mutants suggests that HOP2, MND1, and DMC1 act in the same genetic pathway for the establishment of close juxtaposition between homologous meiotic chromosomes. Biochemical studies indicate that Hop2 and Mnd1 proteins form a stable heterodimer with a higher affinity for double-stranded than single-stranded DNA, and that this heterodimer stimulates the strand assimilation activity of Dmc1 in vitro. Together, the genetic and biochemical results suggest that Hop2, Mnd1, and Dmc1 are functionally interdependent during meiotic DNA recombination.read more
Citations
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Journal ArticleDOI
Mechanism of eukaryotic homologous recombination.
TL;DR: HR accessory factors that facilitate other stages of the Rad51- and Dmc1-catalyzed homologous DNA pairing and strand exchange reaction have also been identified.
Journal ArticleDOI
Mechanism of homologous recombination: mediators and helicases take on regulatory functions
Patrick Sung,Hannah L. Klein +1 more
TL;DR: This work has shown that mutations in the tumour-suppressor protein BRCA2, which has a mediator function in HR, lead to cancer formation and DNA helicases, such as Bloom's syndrome protein, regulate HR at several levels, in attenuating unwanted HR events and in determining the outcome of HR.
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Draft genome sequence and genetic transformation of the oleaginous alga Nannochloropis gaditana.
Randor Radakovits,Robert E. Jinkerson,Susan I. Fuerstenberg,Hongseok Tae,Robert E. Settlage,Jeffrey L. Boore,Matthew C. Posewitz +6 more
TL;DR: It is shown that N. gaditana has highly favourable lipid yields, and is a promising production organism, and the availability of a genome sequence and transformation methods will facilitate investigations into N. Gaditana lipid biosynthesis and permit genetic engineering strategies to further improve this naturally productive alga.
Journal ArticleDOI
Clarifying the mechanics of DNA strand exchange in meiotic recombination
Matthew J. Neale,Scott Keeney +1 more
TL;DR: Many questions about this protein have arisen since its discovery more than a decade ago, but recent genetic and biochemical breakthroughs promise to shed light on the unique behaviours and functions of this central player in the remarkable chromosome dynamics of meiosis.
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Synthesis and Function of Membrane Phosphoinositides in Budding Yeast, Saccharomyces cerevisiae
Thomas Strahl,Jeremy Thorner +1 more
TL;DR: The current state of knowledge about the properties and regulation of the ATP-dependent lipid kinases responsible for synthesis of phosphoinositides and also the additional temporal and spatial controls exerted by the phosphatases and a phospholipase that act on phosphoinposides in yeast are reviewed.
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