Showing papers in "Developmental Cell in 2003"

TL;DR: Two sets of cardiogenic precursors are defined, one of which expresses and requires Isl1 and the other of which does not, which have implications for the development of specific cardiac lineages, left-right asymmetry, cardiac evolution, and isolation of cardiac progenitor cells.

TL;DR: Recent developments in both the functions and mechanisms of noncanonical Wnt signaling are reviewed, and some challenges and difficulties the field faces are outlined.

TL;DR: The authors would like to thank Drs.W.K. van der Klei, Beth Levine, Fulvio Reggiori, and Takahiro Shintani for helpful comments on the manuscript, and the many researchers in the yeast field who have agreed to changes in the standard names of various genes.

TL;DR: The identified microRNAs in undifferentiated and differentiated mouse embryonic stem (ES) cells suggest that miRNAs may have a role in the maintenance of the pluripotent cell state and the regulation of early mammalian development.

TL;DR: The small RNA profile of Drosophila melanogaster is described as a function of development and 178 repeat-associated small interfering RNAs (rasiRNAs) are isolated, suggesting that small RNAs participate in defining chromatin structure.

TL;DR: The basis for TuMV- and other virus-induced disease in plants may be explained, at least partly, by interference with miRNA-controlled developmental pathways that share components with the antiviral RNA-silencing pathway.

TL;DR: Cell transplantation studies suggest that mib function is essential in the signaling cell for efficient activation of Notch in neighboring cells, and observations support a model for Notch activation where the Delta-Notch interaction is followed by endocytosis of Delta and transendocyTosis of the Notch extracellular domain by the signalingcell.

TL;DR: It is proposed that Foxo1 plays an important role in the integration of hormone-activated signaling pathways with the complex transcriptional cascade that promotes adipocyte differentiation.

TL;DR: Npn-1 is a multifunctional receptor that mediates the activities of structurally distinct ligands during development of the heart, vasculature, and nervous system and shows that VEGF-NPN-1 signaling in endothelial cells is required for angiogenesis.

TL;DR: It is shown here that degradation of misfolded glycoprotein substrates requires transcriptional induction of EDEM (ER degradation-enhancing alpha-mannosidase-like protein), and that this is mediated specifically by IRE1-XBP1 and not by ATF6.

TL;DR: Functional analysis demonstrates that Eed-Enx1 is required to establish methylation of histone H3 at lysine 9 and/or lysines 27 on Xi and that this, in turn, is needed to stabilize the Xi chromatin structure.

TL;DR: The segmentation clock is established by Wnt/beta-catenin signaling via a negative-feedback mechanism and that Wnt3a controls the segmentation process in vertebrates.

TL;DR: It is shown that the X and Y chromosomes of histone H2AX-deficient spermatocytes fail to condense to form a sex body, do not initiate MSCI, and exhibit severe defects in meiotic pairing.

TL;DR: Microarray analysis of biofilm-associated bacteria shows that expression of the Vibrio polysaccharide synthesis (vps) operons is enhanced in hapR mutants, suggesting that quorum sensing may promote cellular exit from the biofilm once the organisms have traversed the gastric acid barrier of the stomach.

TL;DR: This review focuses on indicators that use GFP-based fluorescence resonance energy transfer (FRET) technologies and discusses how these methodologies have given insights into biological questions relating to the spatiotemporal patterns of signaling by cAMP, calcium, receptor tyrosine kinases, and other molecules.

TL;DR: This study reveals that, in addition to the pattern recognition receptor-mediated NF-KB-dependent immune response, Drosophila undergoes a complex systemic response that is mediated by the production of cytokines in blood cells, a process that is similar to the acute phase response in mammals.

TL;DR: This review focuses on the most outstanding issues involving NPC structure, assembly, and function.

TL;DR: In the nematode Caenorhabditis elegans, downregulation of the annexin homolog prevents efficient engulfment of pharyngeal cell corpses, which may explain a pathogenic mechanism of chronic inflammatory diseases where annexin I autoantibodies have been described.

TL;DR: It is shown that apoptotic proteins play an essential role during spermatid individualization in Drosophila melanogaster, and that a giant ubiquitin-conjugating enzyme, dBruce, is required to protect the sperm nucleus against hypercondensation and degeneration.

TL;DR: The relationships between assembly of the synaptonemal complex (SC) and progression of recombination between homologous chromosomes during Caenorhabditis elegans meiosis are probed and SYP-2 is identified as a structural component of the SC central region and central region assembly depends on proper morphogenesis of chromosome axes.

TL;DR: The Jun N-terminal kinase (JNK) signaling pathway is introduced as a genetic determinant of aging in Drosophila melanogaster and it is shown that flies with mutations that augment JNK signaling accumulate less oxidative damage and live dramatically longer than wild-type flies.

TL;DR: Microscopy of fluorescent fusion proteins and genetic dependencies show that fission yeast assemble and constrict a cytokinetic contractile ring in a precisely timed, sequential order, providing the framework for analysis of molecular mechanisms.

TL;DR: Transcriptional response of known TTSS genes indicates a hierarchical pattern of expression in which a set of secretion apparatus and regulatory genes is constitutively expressed, and Transcriptome analysis of mutants lacking the ability to synthesize cAMP implicated this pathway in the global regulation of host-directed virulence determinants, including the T TSS.

TL;DR: A model whereby AIP4, Hrs, and Vps4 coordinate a cascade of ubiquitination and deubiquitination events that sort CXCR4 to the degradative pathway is proposed.

TL;DR: Examination of the hb 3'UTR reveals potential binding sites for known fly miRNAs and finds that hbl-1/lin-57 is regulated by let-7, at least in the nervous system, which suggests evolutionary conservation of hunchback genes may include temporal control of cell fate specification and microRNA-mediated regulation.

TL;DR: Although Sox2 expression in the early embryonic CNS appears uniform, it is actually pieced together by five separate enhancers with distinct spatio-temporal specificities, including the one activated by the neural induction signals emanating from Hensen's node.

TL;DR: It is found that a member of the c-Jun NH(2)-terminal protein kinase (JNK) subgroup of MAP kinases, JNK1, is involved in regulation of MT dynamics in neuronal cells.

TL;DR: It is shown that hbl-1, the C. elegans hunchback ortholog, also controls temporal patterning of the nervous system in Drosophila and is a probable target of microRNA regulation through its 3'UTR.

TL;DR: In this paper, the molecular genetic mechanisms of hematopoietic cell fate determination and commitment within Drosophila blood cell lineages are discussed and compared to vertebrate mechanisms.

TL;DR: It is proposed that RacGAP50C-Pavarotti complexes travel on cortical microtubules to the cell equator, where they associate with the Pebble RhoGEF to position contractile ring formation and coordinate F-actin and microtubule remodeling during cytokinesis.