High-resolution Fourier-transform infrared chemical imaging with multiple synchrotron beams
Michael J. Nasse,Michael J. Walsh,Eric C. Mattson,Ruben Reininger,Andre Kajdacsy-Balla,Virgilia Macias,Rohit Bhargava,Carol J. Hirschmugl +7 more
TLDR
This advance allows truly diffraction-limited high-resolution imaging over the entire mid-infrared spectrum with high chemical sensitivity and fast acquisition speed while maintaining high-quality SNR.Abstract:
Conventional Fourier-transform infrared (FTIR) microspectroscopic systems are limited by an inevitable trade-off between spatial resolution, acquisition time, signal-to-noise ratio (SNR) and sample coverage. We present an FTIR imaging approach that substantially extends current capabilities by combining multiple synchrotron beams with wide-field detection. This advance allows truly diffraction-limited high-resolution imaging over the entire mid-infrared spectrum with high chemical sensitivity and fast acquisition speed while maintaining high-quality SNR.read more
Citations
More filters
Journal ArticleDOI
Superoxide Ion: Generation and Chemical Implications
TL;DR: This review comprises the main characteristics of O2(•-) followed by generation methods, and its potential applications including the destruction of hazardous chemicals, synthesis of organic compounds, and many other applications are highlighted.
Journal ArticleDOI
Using Fourier transform IR spectroscopy to analyze biological materials
Matthew J. Baker,Júlio Trevisan,Paul Bassan,Rohit Bhargava,Holly J. Butler,Konrad Matthew Dorling,Peter R. Fielden,Simon W. Fogarty,Nigel J. Fullwood,Kelly A. Heys,Caryn Hughes,Peter Lasch,Pierre L. Martin-Hirsch,Blessing Obinaju,Ganesh D. Sockalingum,Josep Sulé-Suso,Rebecca J. Strong,Michael J. Walsh,Bayden R. Wood,Peter Gardner,Francis Martin +20 more
TL;DR: This manuscript brings together some of the leaders in this field to allow the standardization of methods and procedures for adapting a multistage approach to a methodology that can be applied to a variety of cell biological questions or used within a clinical setting for disease screening or diagnosis.
Journal ArticleDOI
AFM-IR: Technology and Applications in Nanoscale Infrared Spectroscopy and Chemical Imaging
Alexandre Dazzi,Craig Prater +1 more
TL;DR: AFM-IR applications that will be discussed include those in polymers, life sciences, photonics, solar cells, semiconductors, pharmaceuticals, and cultural heritage.
Journal ArticleDOI
Single-Cell Metabolomics: Analytical and Biological Perspectives
TL;DR: The fact that single-cell measurements exhibited a much larger spread in metabolite concentrations than population measurements was exploited to determine many metabolite-metabolite correlations, which were altered in 2DG-treated yeast cells relative to controls.
Journal ArticleDOI
Dual-Band Perfect Absorber for Multispectral Plasmon-Enhanced Infrared Spectroscopy
TL;DR: A dual-band perfect absorber based on a gold nanocross structure with two bands of maximum absorption of 94% is introduced and can be readily tuned throughout the mid-infrared with their associated resonances giving rise to large near-field enhancements.
References
More filters
Journal ArticleDOI
New Advances in the Application of FTIR Microscopy and Spectroscopy for the Characterization of Artistic Materials
TL;DR: The initial results show that far-IR spectroscopy is exceptionally useful with mural paintings or with corrosion products from which larger sample quantities can generally be collected, and the innovative use of IR-transparent salts as embedding material for cross sections can prevent contamination of the embedding resin and improve detection of organic substances.
Journal ArticleDOI
Synchrotron IR spectromicroscopy: chemistry of living cells.
TL;DR: Advanced analytical capabilities of synchrotron IR spectromicroscopy meet the demands of modern biological research for studying molecular reactions in individual living cells.
BookDOI
Spectrochemical Analysis Using Infrared Multichannel Detectors
Rohit Bhargava,Ira W. Levin +1 more
TL;DR: In this article, Bhargava et al. present a detailed overview of the application of near-instrained imagers in agriculture and food processing. But their focus is on the use of a wide range of technologies, such as hard and soft tisSUES.
Journal ArticleDOI
Fourier transform infrared microscopical analysis with synchrotron radiation: The microscope optics and system performance (invited)
TL;DR: In this paper, a Fourier transform infrared micro-spectrometer was interfaced with the National Synchrotron Light Source (NSLSTM) to provide high-quality IR spectra for probe diameters at the diffraction limit.
Journal ArticleDOI
Fourier transform infrared microspectroscopy identifies symmetric PO(2)(-) modifications as a marker of the putative stem cell region of human intestinal crypts.
Michael J. Walsh,Tariq G. Fellous,Azzedine Hammiche,Wey Ran Lin,Nigel J. Fullwood,Olaug Grude,Fariba Bahrami,James M. Nicholson,Marine Cotte,Jean Susini,Hubert M. Pollock,Mairi Brittan,Pierre L. Martin-Hirsch,Malcolm R. Alison,Francis Martin +14 more
TL;DR: νsPO2−, probably associated with DNA conformational alterations, might facilitate a means of identifying stem cells, which may have utility in other tissues where the location of stem cells is unclear, and points to a novel approach of nondestructively identifying a tissue's stem cell location.
Related Papers (5)
Using Fourier transform IR spectroscopy to analyze biological materials
Matthew J. Baker,Júlio Trevisan,Paul Bassan,Rohit Bhargava,Holly J. Butler,Konrad Matthew Dorling,Peter R. Fielden,Simon W. Fogarty,Nigel J. Fullwood,Kelly A. Heys,Caryn Hughes,Peter Lasch,Pierre L. Martin-Hirsch,Blessing Obinaju,Ganesh D. Sockalingum,Josep Sulé-Suso,Rebecca J. Strong,Michael J. Walsh,Bayden R. Wood,Peter Gardner,Francis Martin +20 more