High-resolution Fourier-transform infrared chemical imaging with multiple synchrotron beams
Michael J. Nasse,Michael J. Walsh,Eric C. Mattson,Ruben Reininger,Andre Kajdacsy-Balla,Virgilia Macias,Rohit Bhargava,Carol J. Hirschmugl +7 more
TLDR
This advance allows truly diffraction-limited high-resolution imaging over the entire mid-infrared spectrum with high chemical sensitivity and fast acquisition speed while maintaining high-quality SNR.Abstract:
Conventional Fourier-transform infrared (FTIR) microspectroscopic systems are limited by an inevitable trade-off between spatial resolution, acquisition time, signal-to-noise ratio (SNR) and sample coverage. We present an FTIR imaging approach that substantially extends current capabilities by combining multiple synchrotron beams with wide-field detection. This advance allows truly diffraction-limited high-resolution imaging over the entire mid-infrared spectrum with high chemical sensitivity and fast acquisition speed while maintaining high-quality SNR.read more
Citations
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Journal ArticleDOI
Dynamic full-field infrared imaging with multiple synchrotron beams.
Eli Stavitski,Randy J. Smith,Megan W. Bourassa,Alvin S. Acerbo,G. L. Carr,Lisa M. Miller,Lisa M. Miller +6 more
TL;DR: High chemical sensitivity, rapid acquisition times, and superior signal-to-noise characteristics of the instrument are demonstrated, and the unique characteristics of this setup enabled the real-time study of heterogeneous chemical dynamics with diffraction-limited spatial resolution for the first time.
Journal ArticleDOI
Synchrotron infrared nano-spectroscopy and -imaging
TL;DR: Synchrotron infrared nano-spectroscopy (SINS) as discussed by the authors is a recent technique for near-field infrared measurements across the mid-to far-infrared with nanometer spatial resolution.
Journal ArticleDOI
Chemical structure and morphology of dorsal root ganglion neurons from naive and inflamed mice.
TL;DR: In this paper, a ring-shaped distribution of lipid and/or carbohydrate enrichment in subpopulations of neurons has been identified, which has never before been reported, indicating that the inflammatory-mediated increase in lipid content is largely dependent on TRPA1.
Journal ArticleDOI
Hyperspectral Tissue Image Segmentation Using Semi-Supervised NMF and Hierarchical Clustering
Neeraj Kumar,Phanikrishna Uppala,Karthik Duddu,Hari Sreedhar,Vishal K. Varma,Grace Guzman,Michael J. Walsh,Amit Sethi +7 more
TL;DR: An HSI pixel classification method that uses semi-supervised learning for both spectral dimension reduction and hierarchical pixel clustering is developed and it is shown that disease classification is straightforward after HSI image segmentation.
Journal ArticleDOI
Toward optimal spatial and spectral quality in widefield infrared spectromicroscopy of IR labelled single cells.
Eric C. Mattson,Miriam Unger,Sylvain Clède,Sylvain Clède,Sylvain Clède,François Lambert,François Lambert,François Lambert,Clotilde Policar,Clotilde Policar,Clotilde Policar,Asher Imtiaz,Roshan M. D'Souza,Carol J. Hirschmugl +13 more
TL;DR: The effects of magnification, spatial oversampling, spectral pre-processing and deconvolution are demonstrated, focusing on the intracellular detection and distribution of an exogenous metal tris-carbonyl derivative 1 in a single MDA-MB-231 breast cancer cell.
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