High-resolution Fourier-transform infrared chemical imaging with multiple synchrotron beams
Michael J. Nasse,Michael J. Walsh,Eric C. Mattson,Ruben Reininger,Andre Kajdacsy-Balla,Virgilia Macias,Rohit Bhargava,Carol J. Hirschmugl +7 more
TLDR
This advance allows truly diffraction-limited high-resolution imaging over the entire mid-infrared spectrum with high chemical sensitivity and fast acquisition speed while maintaining high-quality SNR.Abstract:
Conventional Fourier-transform infrared (FTIR) microspectroscopic systems are limited by an inevitable trade-off between spatial resolution, acquisition time, signal-to-noise ratio (SNR) and sample coverage. We present an FTIR imaging approach that substantially extends current capabilities by combining multiple synchrotron beams with wide-field detection. This advance allows truly diffraction-limited high-resolution imaging over the entire mid-infrared spectrum with high chemical sensitivity and fast acquisition speed while maintaining high-quality SNR.read more
Citations
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Angle-and polarization-dependent collective excitation of plasmonic nanoarrays for surface enhanced infrared spectroscopy
Vladimir Liberman,Ronen Adato,Alket Mertiri,Ahmet Ali Yanik,Kai Chen,Thomas H. Jeys,Shyamsunder Erramilli,Hatice Altug +7 more
TL;DR: This work considers computationally the impact of a microscope objective illumination cone on array performance and derives an approach for computing angular- and spatially-averaged reflectance for various numerical aperture (NA) objectives to show that arrays that are perfectly optimized for normal incidence undergo significant response degradation even at modest NAs.
Journal ArticleDOI
High‐Resolution Label‐Free Detection of Biocompatible Polymeric Nanoparticles in Cells
Elisabetta Pancani,Jérémie Mathurin,Sylvie Bilent,Marie-Françoise Bernet-Camard,Alexandre Dazzi,Ariane Deniset-Besseau,Ruxandra Gref +6 more
TL;DR: A reliable, nondestructive, and direct method able to precisely locate and chemically characterize PLA NPs within a cell without the need of labeling is presented.
Journal ArticleDOI
Infrared imaging of small molecules in living cells: from in vitro metabolic analysis to cytopathology
TL;DR: The molecular detail obtained from this analysis allows its use in evaluating the pharmacological effect of inhibitors of glycolytic enzymes with potential consequences for in vitro drug testing, and the implications of the spectral contribution from cellular metabolites on applications in IR spectral cytopathology (SCP).
Journal ArticleDOI
Synchrotron radiation infrared microspectroscopy of single living cells in microfluidic devices: advantages, disadvantages and future perspectives
TL;DR: In this article, the technical solutions employed for microfabricating completely sealed IR-visible transparent fluidic devices for living cell analysis are presented, and several examples of the results obtained in the study of living U937 monocytes subjected to different stimuli are selected for highlighting both the advantages and the disadvantages offered by their approach for cellular biology.
Journal ArticleDOI
High-definition Fourier Transform Infrared (FT-IR) spectroscopic imaging of human tissue sections towards improving pathology.
Hari Sreedhar,Vishal K. Varma,Peter L. Nguyen,Bennett Davidson,Sanjeev Akkina,Grace Guzman,Suman Setty,Andre Kajdacsy-Balla,Michael J. Walsh +8 more
TL;DR: How to obtain IR images from human tissues using an FT-IR system, how to modify existing instrumentation to allow for high-definition imaging capabilities, and how to visualizeFT-IR images are shown.
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