High-resolution Fourier-transform infrared chemical imaging with multiple synchrotron beams
Michael J. Nasse,Michael J. Walsh,Eric C. Mattson,Ruben Reininger,Andre Kajdacsy-Balla,Virgilia Macias,Rohit Bhargava,Carol J. Hirschmugl +7 more
TLDR
This advance allows truly diffraction-limited high-resolution imaging over the entire mid-infrared spectrum with high chemical sensitivity and fast acquisition speed while maintaining high-quality SNR.Abstract:
Conventional Fourier-transform infrared (FTIR) microspectroscopic systems are limited by an inevitable trade-off between spatial resolution, acquisition time, signal-to-noise ratio (SNR) and sample coverage. We present an FTIR imaging approach that substantially extends current capabilities by combining multiple synchrotron beams with wide-field detection. This advance allows truly diffraction-limited high-resolution imaging over the entire mid-infrared spectrum with high chemical sensitivity and fast acquisition speed while maintaining high-quality SNR.read more
Citations
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Novel bio-spectroscopic imaging reveals disturbed protein homeostasis and thiol redox with protein aggregation prior to hippocampal CA1 pyramidal neuron death induced by global brain ischemia in the rat.
Mark J. Hackett,Shari E. Smith,Sally Caine,Helen Nichol,Graham N. George,Ingrid J. Pickering,Phyllis G. Paterson +6 more
TL;DR: This study has provided direct support for a correlative mechanistic link in both spatial and temporal domains between oxidative stress, protein aggregation and altered protein homeostasis prior to irreparable neuron damage following global ischemia.
Journal ArticleDOI
Enhanced spectral histology in the colon using high-magnification benchtop FTIR imaging
TL;DR: In this paper, the authors used vibrational spectroscopic imaging to study the histo-pathological features and correlated the alterations were correlated to a disease state, and showed that high power mid-IR radiation sources would be beneficial, especially when measuring in rapid clinically relevant timescales.
Journal ArticleDOI
Far-Field Super-Resolution Vibrational Spectroscopy.
Christian T. Graefe,David Punihaole,Celina M. Harris,Michael J. Lynch,Ryan Leighton,Renee R. Frontiera +5 more
TL;DR: Efforts to develop super-resolution techniques based on vibrational spectroscopies and address possible sample applications as well as future potential resolution enhancements are discussed.
Journal ArticleDOI
High-spatial-resolution mapping of superhydrophobic cicada wing surface chemistry using infrared microspectroscopy and infrared imaging at two synchrotron beamlines
Mark J. Tobin,Ljiljana Puskar,Jafar Hasan,Hayden K. Webb,Carol J. Hirschmugl,Michael J. Nasse,Michael J. Nasse,Gediminas Gervinskas,Saulius Juodkazis,Gregory S. Watson,Jolanta A. Watson,Russell J. Crawford,Elena P. Ivanova +12 more
TL;DR: The chemical composition of the wings of the Clanger cicada were characterized using infrared (IR) microspectroscopy, showing that, while the protein component was homogeneously distributed, a significant degree of heterogeneity was observed in the distribution of the waxy component, which may contribute to the self-cleaning and aerodynamic properties of the cicADA wing.
Journal ArticleDOI
Shining a new light into molecular workings
TL;DR: A technique to substantially increase the resolution and imaging area of Fourier-transform infrared microspectroscopy, while decreasing the amount of time required for image acquisition, may augment the use of this technology in biomedical and environmental research.
References
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Dirac charge dynamics in graphene by infrared spectroscopy
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