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Open AccessJournal ArticleDOI

High-resolution Fourier-transform infrared chemical imaging with multiple synchrotron beams

TLDR
This advance allows truly diffraction-limited high-resolution imaging over the entire mid-infrared spectrum with high chemical sensitivity and fast acquisition speed while maintaining high-quality SNR.
Abstract
Conventional Fourier-transform infrared (FTIR) microspectroscopic systems are limited by an inevitable trade-off between spatial resolution, acquisition time, signal-to-noise ratio (SNR) and sample coverage. We present an FTIR imaging approach that substantially extends current capabilities by combining multiple synchrotron beams with wide-field detection. This advance allows truly diffraction-limited high-resolution imaging over the entire mid-infrared spectrum with high chemical sensitivity and fast acquisition speed while maintaining high-quality SNR.

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Citations
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Journal ArticleDOI

Retinal oxidative stress at the onset of diabetes determined by synchrotron FTIR widefield imaging: towards diabetes pathogenesis.

TL;DR: The spectral biomarkers and the spatial biochemical alterations in the diabetic retina and in specific layers were identified for the first time and will help to bridge the gap in the understanding of the molecular and cellular mechanisms that contribute to the pathobiology of diabetic retinopathy.
Journal ArticleDOI

Raman and mid-infrared spectroscopic imaging: applications and advancements

TL;DR: In this paper, the authors discuss the instrumentation, applications and latest advancements of Raman and MIR spectroscopic imaging in various fields, ranging from materials science to medicine and illustrate with specific examples from their recent research.
Journal ArticleDOI

Single-shot mid-infrared incoherent holography using Lucy-Richardson-Rosen algorithm

TL;DR: Lucy-Richardson-Rosen algorithm has been proposed in this article for 3D image reconstruction without two-beam interference (TBII) by using deterministic fields.
Journal ArticleDOI

Synchrotron based infrared imaging and spectroscopy via focal plane array on live fibroblasts in D2O enriched medium

TL;DR: The viability of using synchrotron-based full-field infrared imaging to study biochemical processes inside living cells is successfully tested and a description of cellular biochemistry and biophysics that contains both spatial and temporal information is given.
Journal ArticleDOI

Opportunities and challenges for polymer science using synchrotron-based infrared spectroscopy

TL;DR: In this paper, the origin and nature of synchrotron infrared microspectroscopy is described, highlighting some of the key developments that will make a future impact in the study of macromolecular materials and illustrating several applications in the area of polymer science.
References
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BookDOI

Handbook of biological confocal microscopy

TL;DR: Methods for Three-Dimensional Imaging and Tutorial on Practical Confocal Microscopy and Use of the Confocal Test Specimen.
Journal ArticleDOI

Dirac charge dynamics in graphene by infrared spectroscopy

TL;DR: In this article, an infrared spectromicroscopy study of charge dynamics in graphene integrated in gated devices is presented, which reveals significant departures of the quasiparticle dynamics from predictions made for Dirac fermions in idealized, free-standing graphene.
Journal ArticleDOI

Near-field probing of vibrational absorption for chemical microscopy

TL;DR: In this article, the authors demonstrate the use of the apertureless approach to scan near field optical microscopy to obtain contrast in vibrational absorption on a scale of about 100 nanometres, about one-hundredth of a wavelength.
Journal ArticleDOI

Sea urchin spine calcite forms via a transient amorphous calcium carbonate phase.

TL;DR: It is shown that sea urchin spine regeneration proceeds via the initial deposition of amorphous calcium carbonate, which may have interesting implications for the development of sophisticated materials.
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