Journal ArticleDOI
Human oestrogen receptor cDNA: sequence, expression and homology to v-erb-A
Stephen Green,P Walter,Vijay Kumar,Andrée Krust,Jean-Marc Bornert,Patrick Argos,Pierre Chambon +6 more
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TLDR
Cloned and sequenced the complete complementary DNA of the oestrogen receptor (ER) present in the breast cancer cell line MCF-7 and found extensive homology between the ER and the erb-A protein of the oncogenic avian erythroblastosis virus.Abstract:
We have cloned and sequenced the complete complementary DNA of the oestrogen receptor (ER) present in the breast cancer cell line MCF-7. The expression of the ER cDNA in HeLa cells produces a protein that has the same relative molecular mass and binds oestradiol with the same affinity as the MCF-7 ER. There is extensive homology between the ER and the erb-A protein of the oncogenic avian erythroblastosis virus.read more
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The steroid and thyroid hormone receptor superfamily
TL;DR: A superfamily of regulatory proteins that include receptors for thyroid hormone and the vertebrate morphogen retinoic acid is identified, suggesting mechanisms underlying morphogenesis and homeostasis may be more ubiquitous than previously expected.
Journal ArticleDOI
The nuclear receptor superfamily: the second decade.
David J. Mangelsdorf,Carl S. Thummel,Miguel Beato,Peter Herrlich,Günther Schütz,Kazuhiko Umesono,Bruce Blumberg,Philippe Kastner,Manuel Mark,Pierre Chambon,Ronald M. Evans +10 more
TL;DR: This research presents a new probabilistic procedure called ‘spot-spot analysis’ to characterize the response of the immune system to the presence of E.coli.
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Activation of a member of the steroid hormone receptor superfamily by peroxisome proliferators
Isabelle Issemann,Stephen Green +1 more
TL;DR: A member of the steroid hormone receptor superfamily of ligand-activated transcription factors is cloned that is activated by a diverse class of rodent hepatocarcinogens that causes proliferation of peroxisomes.
Journal ArticleDOI
Gene regulation by steroid hormones.
TL;DR: The location, orientation, and structure of the hormone regulatory elements (HRE) in nine hormonally modulated genes is described and a model for the interaction is proposed in which a dimer of the receptor in head-to-head orientation binds to the inverted symmetry element of the HRE.
Journal ArticleDOI
ERβ: Identification and characterization of a novel human estrogen receptor
TL;DR: A novel estrogen receptor (hereinafter referred to as ERβ) was cloned using degenerate PCR primers and revealed that ERβ is expressed in human thymus, spleen, ovary and testis, and the level of transactivation by 17β‐estradiol is higher for ERα than for ERβ, which may reflect suboptimal conditions for ER β at thelevel of the ligand, responsive element or cellular context.
References
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A simple method for displaying the hydropathic character of a protein
Jack Kyte,Russell F. Doolittle +1 more
TL;DR: A computer program that progressively evaluates the hydrophilicity and hydrophobicity of a protein along its amino acid sequence has been devised and its simplicity and its graphic nature make it a very useful tool for the evaluation of protein structures.
Journal ArticleDOI
Compilation and analysis of sequences upstream from the translational start site in eukaryotic mRNAs
TL;DR: The sequence CCAGCCAUG (G) thus emerges as a consensus sequence for eukaryotic initiation sites, and the extent to which the ribosome binding site in a given mRNA matches the -1 to -5 consensus sequence varies.
Journal ArticleDOI
Human epidermal growth factor receptor cDNA sequence and aberrant expression of the amplified gene in A431 epidermoid carcinoma cells
Axel Ullrich,Lisa M. Coussens,Joel S. Hayflick,Thomas J. Dull,Alane M. Gray,A W Tam,Jeffrey E. Lee,Yosef Yarden,Towia A. Libermann,Joseph Schlessinger +9 more
TL;DR: The complete 1,210-amino acid sequence of the human epidermal growth factor (EGF) receptor precursor, deduced from cDNA clones derived from placental and A431 carcinoma cells, reveals close similarity between the entire predicted ν-erb-B mRNA oncogene product and the receptor transmembrane and cytoplasmic domains.
Journal ArticleDOI
A short amino acid sequence able to specify nuclear location
TL;DR: By reducing the size of the transposed sequence, it is concluded that Pro-Lys- lys- Lys-Arg-L Lys-Val can act as a nuclear location signal and may represent a prototype of similar sequences in other nuclear proteins.