Identification, modeling and ligand affinity of early deuterostome CYP51s, and functional characterization of recombinant zebrafish sterol 14α-demethylase
Ann Michelle Stanley Morrison,Jared V. Goldstone,David C. Lamb,Akira Kubota,Benjamin Lemaire,John J. Stegeman +5 more
TLDR
Biochemical and computational analyses are consistent with lanosterol being a substrate for early deuterostome CYP51s, and the results expand the phylogenetic view of animal CYP 51, with evolutionary, environmental and therapeutic implications.About:
This article is published in Biochimica et Biophysica Acta.The article was published on 2014-06-01 and is currently open access. It has received 26 citations till now. The article focuses on the topics: Lanosterol & Strongylocentrotus purpuratus.read more
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Occurrence, fate and ecological risk of five typical azole fungicides as therapeutic and personal care products in the environment: A review.
TL;DR: The reported effects include regulation changes in expression of cytochrome P450-related genes and alteration in CYP450-regulated steroidogenesis causing endocrine disruption in fish causing toxic effects on aquatic organisms such as algae and fish.
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The developmental effect of difenoconazole on zebrafish embryos: A mechanism research.
Mu Xiyan,Tingting Chai,Kai Wang,Lizhen Zhu,Ying Huang,Gongming Shen,Yingren Li,Xuefeng Li,Chengju Wang +8 more
TL;DR: A large suite of symptoms were induced by difenoconazole, including hatching regression, heart rate decrease, growth inhibition and teratogenic effects, and Q-PCR (quantitative real-time polymerase chain reaction) results showed that the transcription of genes related to embryonic development was decreased after exposure.
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Occurrence and origin of sensitivity toward difenoconazole in zebrafish (Danio reio) during different life stages.
TL;DR: An investigation of the mechanisms contributing to the divergent sensitivity toward the triazole fungicide difenoconazole of zebrafish (Danio reio) during different life stages finds a decrease in antioxidant enzyme activities was observed in both the embryos and the livers of adult fish after exposure to difanoconazoles.
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Exposure to prothioconazole induces developmental toxicity and cardiovascular effects on zebrafish embryo.
TL;DR: The adverse effects on the developmental and cardiovascular system of zebrafish embryo caused by prothioconazole are revealed and a theoretical foundation for pesticide risk management measures is provided.
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Two azole fungicides (carcinogenic triadimefon and non-carcinogenic myclobutanil) exhibit different hepatic cytochrome P450 activities in medaka fish.
TL;DR: In this paper, a lower vertebrate, medaka fish (Oryzias latipes) was exposed to continuous aqueous solutions of carcinogenic triadimefon and non-carcinogenic myclobutanil for 7 to 20 days at sub-lethal or environmentally relevant concentrations and assessed hepatic CYP activity and gene expression associated with CYP-mediated toxicity.
References
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Clustal W and Clustal X version 2.0
Mark A. Larkin,Gordon Blackshields,Nigel P. Brown,R. Chenna,Paul A. McGettigan,Hamish McWilliam,Franck Valentin,Iain M. Wallace,Andreas Wilm,Rodrigo Lopez,J.D. Thompson,Toby J. Gibson,Desmond G. Higgins +12 more
TL;DR: The Clustal W and ClUSTal X multiple sequence alignment programs have been completely rewritten in C++ to facilitate the further development of the alignment algorithms in the future and has allowed proper porting of the programs to the latest versions of Linux, Macintosh and Windows operating systems.
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PROCHECK: a program to check the stereochemical quality of protein structures
TL;DR: The PROCHECK suite of programs as mentioned in this paper provides a detailed check on the stereochemistry of a protein structure and provides an assessment of the overall quality of the structure as compared with well refined structures of the same resolution.
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AutoDock Vina: Improving the speed and accuracy of docking with a new scoring function, efficient optimization, and multithreading
Oleg Trott,Arthur J. Olson +1 more
TL;DR: AutoDock Vina achieves an approximately two orders of magnitude speed‐up compared with the molecular docking software previously developed in the lab, while also significantly improving the accuracy of the binding mode predictions, judging by tests on the training set used in AutoDock 4 development.
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MrBayes 3.2: Efficient Bayesian Phylogenetic Inference and Model Choice across a Large Model Space
Fredrik Ronquist,Maxim Teslenko,Paul van der Mark,Daniel L. Ayres,Aaron E. Darling,Sebastian Höhna,Bret Larget,Liang Liu,Marc A. Suchard,John P. Huelsenbeck +9 more
TL;DR: The new version provides convergence diagnostics and allows multiple analyses to be run in parallel with convergence progress monitored on the fly, and provides more output options than previously, including samples of ancestral states, site rates, site dN/dS rations, branch rates, and node dates.
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RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models
TL;DR: UNLABELLED RAxML-VI-HPC (randomized axelerated maximum likelihood for high performance computing) is a sequential and parallel program for inference of large phylogenies with maximum likelihood (ML) that has been used to compute ML trees on two of the largest alignments to date.