Journal ArticleDOI
Identification of a male-specific amplified fragment length polymorphism (AFLP) marker in Broussonetia papyrifera
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TLDR
The present study exhibits amplified fragment length polymorphism (AFLP) molecular marker for sex identification in Broussonetia papyrifera based on nine selective amplification primer combinations and indicates that common homology sequence is existed in both male and female plants.Abstract:
The present study exhibits amplified fragment length polymorphism (AFLP) molecular marker for sex identification in Broussonetia papyrifera . Based on nine selective amplification primer combinations, 230 bands were produced and the E -AGG/M-CAA combination was found to be a male-specific AFLP marker. Subsequently, this male-specific AFLP fragment was sequenced and converted into a sequence tagged site (STS) marker. Based on STS sequence, two primers, MADB-1 and MADB-2 (Male-Associated DNA from B. papyrifera ), were designed to verify the specificity of the fragment. The results indicate that common homology sequence is existed in both male and female plants while one of the bands amplified via MADB-2 primer was solely present in male individuals at high annealing temperature up to 66°C. Finally, MADB-2 primer was introduced to amplify another 16 plants and it revealed that this primer could be used as a convenient, efficient, reliable, and low-cost molecular marker for sex identification in B. papyrifera . Keywords: Broussonetia papyrifera , AFLP, STS, male-specific markerread more
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Review on different mechanisms of sex determination and sex-linked molecular markers in dioecious crops: a current update
TL;DR: The present review emphasizes the mode of sex determination among dioecious plants vis-a-vis summarizes the works related to gender specific markers generated using male and female plants from agriculturally important dioemious crops.
Journal ArticleDOI
Sex Distribution of Paper Mulberry (Broussonetia papyrifera) in the Pacific.
Johany Peñailillo,Gabriela Olivares,Ximena Moncada,Claudia Payacán,Chi-Shan Chang,Kuo-Fang Chung,Peter J. Matthews,Andrea Seelenfreund,Daniela Seelenfreund +8 more
TL;DR: Most paper mulberry plants now present in the Pacific appear to be descended from female clones introduced prehistorically, with the presence of male and female plants in Near and Remote Oceania thought to reflect a dual origin.
Journal ArticleDOI
Generation and validation of unique male sex-specific sequence tagged sites (STS) marker from diverse genotypes of dioecious Jojoba-Simmondsia chinensis (Link) Schneider
TL;DR: Two unique ISSR markers, viz.
Journal ArticleDOI
De Novo assembly of expressed transcripts and global transcriptomic analysis from seedlings of the paper mulberry (Broussonetia kazinoki x Broussonetia papyifera).
TL;DR: This global expression analysis provided novel insights about the molecular mechanisms of the biosynthesis of flavonoid, lignin and cellulose, as well as on the response to biotic and abiotic stresses including the remediation of contaminated soil by the paper mulberry.
Journal ArticleDOI
Phylogeography of herbarium specimens of asexually propagated paper mulberry [Broussonetia papyrifera (L.) L'Hér. ex Vent. (Moraceae)] reveals genetic diversity across the Pacific.
Claudia Payacán,Ximena Moncada,Gloria Rojas,Andrew Clarke,Kuo-Fang Chung,Robin G. Allaby,Daniela Seelenfreund,Andrea Seelenfreund +7 more
TL;DR: Insight is gained into the dispersal of paper mulberry into Oceania through the genetic analysis of herbaria samples which represent a more complete coverage of the historical geographical range of the species in the Pacific before later introductions and local extinctions occurred.
References
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Journal ArticleDOI
Antioxidant Lignans from the Fruits of Broussonetia papyrifera.
TL;DR: Nine new lignans, chushizisins A-I (1-9), and three known lignANs, threo-1-(4-hydroxy-3-methoxyphenyl)-2-{4-[(E)-3-hydroxymethyl-2,3-dihydro-1-benzofuran-5-yl]propan- 1-ol (12), were isolated from the fruits of Brou
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Equilibrium and Kinetic Studies on Biosorption of Heavy Metals by Leaf Powder of Paper Mulberry (Broussonetia papyrifera)
TL;DR: Paper mulberry (Broussonetia papyrifera) leaf powder was used to remove heavy metal ions from aqueous solutions as discussed by the authors, and the specific uptakes of Cu, Pb, and Cd by the leaf powder were 43.40
Journal ArticleDOI
Application of inter simple sequence repeat (ISSR) polymorphism for detection of sex-specific molecular markers in hop (Humulus lupulus L.)
T. V. Danilova,Gennady I. Karlov +1 more
TL;DR: The applicability of ISSR-PCR analysis for development of sex molecular markers in hop is discussed and a high homology of male specific hop DNA sequences to expressed sequences from EMBL plants EST database was found.
Journal ArticleDOI
Sex-specific SCAR markers in the dioecious plant Rumex nivalis (Polygonaceae) and implications for the evolution of sex chromosomes.
TL;DR: SCAR primers based on isolated and sequenced male-specific fragments as identified in an AFLP analysis of the dioecious plant Rumex nivalis resulted in fragments exclusively present in males, in accordance with proposed modes of the evolution of sex chromosomes.
Journal ArticleDOI
Traditional Trees of Pacific Islands: Their Culture, Environment, and Use (review)
TL;DR: Traditional Trees of Pacific Islands is perhaps a new wave of pronounced ethnobotanical emphasis combined with what is basically an identification guide with fairly detailed ecological notes, and will demystify some of the more frequently encountered tree species from coast to mountain.
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