Identification of genes induced in Vibrio cholerae in a dynamic biofilm system
Andrea Seper,Katharina Pressler,Ankunda T. Kariisa,Andrea G. Haid,Sandro Roier,Deborah R. Leitner,Joachim Reidl,Rita Tamayo,Stefan Schild +8 more
TLDR
A biofilm system with constant medium flow and a temporal controlled reporter-system of transcription was used to identify genes induced during dynamic biofilm formation and identified genes known or predicted to be involved in c-di-GMP signaling, motility and chemotaxis, metabolism, and transport.About:
This article is published in International Journal of Medical Microbiology.The article was published on 2014-07-01 and is currently open access. It has received 29 citations till now. The article focuses on the topics: Biofilm matrix & Vibrio cholerae.read more
Citations
More filters
Journal ArticleDOI
Vibrio cholerae Biofilms and Cholera Pathogenesis
Anisia J. Silva,Jorge A. Benitez +1 more
TL;DR: The evidence for biofilm formation during infection, the coordinate regulation of biofilm and virulence gene expression, and the host signals that favor V. cholerae biofilms in pathogenicity are reviewed.
Journal ArticleDOI
Vibrio cholerae Combines Individual and Collective Sensing to Trigger Biofilm Dispersal
Praveen K. Singh,Sabina Bartalomej,Raimo Hartmann,Hannah Jeckel,Lucia Vidakovic,Carey D. Nadell,Knut Drescher,Knut Drescher +7 more
TL;DR: Using a combination of genetic and novel single-cell imaging approaches, it is shown that Vibrio cholerae integrates dual sensory inputs to control the dispersal response: cells use the general stress response, which can be induced via starvation, and they also integrate information about the local cell density and molecular transport conditions in the environment via the quorum sensing apparatus.
Journal ArticleDOI
Virulence Factors Produced by Staphylococcus aureus Biofilms Have a Moonlighting Function Contributing to Biofilm Integrity.
Alexander C. Graf,Anne Leonard,Manuel Schäuble,Lisa M. Rieckmann,Juliane Hoyer,Sandra Maass,Michael Lalk,Dörte Becher,Jan Pané-Farré,Katharina Riedel +9 more
TL;DR: It was revealed that this stabilizing effect is mediated by the strong positive charge of alkaline virulence factors and ribosomal proteins in an acidic ECM environment, which is caused by the release of fermentation products like formate, lactate, and acetate because of oxygen limitation in biofilms.
Journal ArticleDOI
Comparative analyses of biofilm formation among different Cutibacterium acnes isolates.
Torben Kuehnast,Fatih Cakar,Theresa Weinhäupl,Andreas Pilz,Sanja Selak,M. Alexander Schmidt,Christian Rüter,Stefan Schild +7 more
TL;DR: A first comparative analysis of 58 diverse skin- or implant-isolates covering all six C. acnes phylotypes suggests that biofilm formation correlates with the phylotype, rather than the anatomical isolation site, and that extracellular DNA and proteins are critical for adhesion to abiotic surfaces.
Journal ArticleDOI
ToxR Antagonizes H-NS Regulation of Horizontally Acquired Genes to Drive Host Colonization.
TL;DR: ToxR’s genome-wide DNA-binding profile is determined and it is demonstrated that ToxR is a global regulator of both progenitor genome-encoded genes and horizontally acquired islands that encode V. cholerae’'s major virulence factors and define pandemic lineages.
References
More filters
Journal ArticleDOI
Studies on transformation of Escherichia coli with plasmids
Douglas Hanahan,Douglas Hanahan +1 more
TL;DR: Competition with both transforming and non-transforming plasmids indicates that each cell is capable of taking up many DNA molecules, and that the establishment of a transformation event is neither helped nor hindered significantly by the presence of multiple plasmid molecules.
Journal ArticleDOI
From genomics to chemical genomics: new developments in KEGG
Minoru Kanehisa,Susumu Goto,Masahiro Hattori,Kiyoko F. Aoki-Kinoshita,Masumi Itoh,Shuichi Kawashima,Toshiaki Katayama,Michihiro Araki,Mika Hirakawa +8 more
TL;DR: The scope of KEGG LIGAND has been significantly expanded to cover both endogenous and exogenous molecules, and RPAIR contains curated chemical structure transformation patterns extracted from known enzymatic reactions, which would enable analysis of genome-environment interactions.
Journal ArticleDOI
A novel suicide vector and its use in construction of insertion mutations: osmoregulation of outer membrane proteins and virulence determinants in Vibrio cholerae requires toxR.
TL;DR: Certain environmental signals (i.e., osmolarity and the presence of amino acids) are tightly coupled to the expression of toxR-regulated proteins and therefore may be signals that are directly sensed by the ToxR protein.
Journal ArticleDOI
Physiological heterogeneity in biofilms.
TL;DR: The processes that generate chemical gradients inBiofilms, the genetic and physiological responses of the bacteria as they adapt to these gradients and the techniques that can be used to visualize and measure the microscale physiological heterogeneities of bacteria in biofilms are discussed.
Journal ArticleDOI
Quantification of biofilm structures by the novel computer program COMSTAT.
Arne Heydorn,Alex Toftgaard Nielsen,Morten Hentzer,Claus Sternberg,Michael Givskov,Bjarne Kjær Ersbøll,Søren Molin +6 more
TL;DR: Analysis of biofilms of P. aureofaciens growing on 0.03 mM, 0.1 mM or 0.5 mM citrate minimal media showed that mean biofilm thickness increased with increasing citrate concentration, whereas surface to volume ratio increased with higher citrate concentrations.