Improving Viability of Stem Cells During Syringe Needle Flow Through the Design of Hydrogel Cell Carriers

TLDR: Experimental and theoretical data suggest that extensional flow at the entrance of the syringe needle is the main cause of acute cell death, and mechanistic insight into the role of mechanical forces during cell delivery is provided and support the use of protective hydrogels in future clinical stem cell injection studies.

Abstract: Cell transplantation is a promising therapy for a myriad of debilitating diseases; however, current delivery protocols using direct injection result in poor cell viability. We demonstrate that during the actual cell injection process, mechanical membrane disruption results in significant acute loss of viability at clinically relevant injection rates. As a strategy to protect cells from these damaging forces, we hypothesize that cell encapsulation within hydrogels of specific mechanical properties will significantly improve viability. We use a controlled in vitro model of cell injection to demonstrate success of this acute protection strategy for a wide range of cell types including human umbilical vein endothelial cells (HUVEC), human adipose stem cells, rat mesenchymal stem cells, and mouse neural progenitor cells. Specifically, alginate hydrogels with plateau storage moduli (G′) ranging from 0.33 to 58.1 Pa were studied. A compliant crosslinked alginate hydrogel (G′=29.6 Pa) yielded the highest HUVEC vi...