Intrinsic stoichiometric equilibrium constants for the binding of zinc(II) and copper(II) to the high affinity site of serum albumin.
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TLDR
Comparison of binding strengths was possible only through the calculation of an intrinsic stoichiometric binding constant and it was suggested that while both histidyl and carboxyl groups appear to be involved in copper binding, Histidyl residues alone were sufficient for zinc binding.About:
This article is published in Journal of Biological Chemistry.The article was published on 1993-10-15 and is currently open access. It has received 216 citations till now. The article focuses on the topics: Bovine serum albumin & Binding constant.read more
Citations
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Journal ArticleDOI
The cellular prion protein binds copper in vivo
David R. Brown,Kefeng Qin,Jochen Herms,Axel Madlung,Jean Manson,Robert Strome,Paul E. Fraser,T. P. A. Kruck,A von Bohlen,Walter J. Schulz-Schaeffer,Armin Giese,David Westaway,Hans A. Kretzschmar +12 more
TL;DR: Findings indicate that PrPC can exist in a Cu-metalloprotein form in vivo, and that its amino terminus contains the octapeptide PHGGGWGQ, which is among the best-preserved regions of mammalian PrPC.
Journal ArticleDOI
Human serum albumin: from bench to bedside.
TL;DR: HSA is a valuable biomarker of many diseases, including cancer, rheumatoid arthritis, ischemia, post-menopausal obesity, severe acute graft-versus-host disease, and diseases that need monitoring of the glycemic control.
Journal ArticleDOI
Dramatic aggregation of Alzheimer abeta by Cu(II) is induced by conditions representing physiological acidosis.
Craig S. Atwood,Robert D. Moir,Xudong Huang,Richard C. Scarpa,N. M. E. Bacarra,Donna M. Romano,Mariana A. Hartshorn,Rudolph E. Tanzi,Ashley I. Bush +8 more
TL;DR: Results indicate that H+-induced conformational changes unmask a metal-binding site on Aβ that mediates reversible assembly of the peptide that explains the recently reported enrichment of these metal ions in amyloid plaques in Alzheimer's disease.
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Trace elements in human physiology and pathology. Copper.
TL;DR: In biological systems, copper homeostasis has been characterized at the molecular level and is coordinated by several proteins such as glutathione, metallothionein, Cu-transporting P-type ATPases, Menkes and Wilson proteins and by cytoplasmic transport proteins called copper chaperones to ensure that it is delivered to specific subcellular compartments and thereby to copper-requiring proteins.
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Structural and immunologic characterization of bovine, horse, and rabbit serum albumins.
Karolina A. Majorek,Przemyslaw J. Porebski,Arjun Dayal,Matthew D. Zimmerman,Kamila Jablonska,Alan J. Stewart,Maksymilian Chruszcz,Wladek Minor +7 more
TL;DR: In this paper, the crystal structures of albumins from cattle (BSA), horse (ESA) and rabbit (RSA) sera were analyzed in the context of their potential allergenicity and cross-reactivity.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
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The Attractions of Proteins for Small Molecules and Ions
TL;DR: The number and variety of known compounrjs between proteins and small molecules are increasing rapidly and make a fascinating story as discussed by the authors, and there are many compounds of serum albumin, which was used during the war by many chemists, most of whom found at least one 6ew compound.
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Atomic structure and chemistry of human serum albumin.
Xiao Min He,Daniel C. Carter +1 more
TL;DR: The three-dimensional structure of human serum albumin has been determined crystallographically to a resolution of 2.8 Å and should provide insight into future pharmacokinetic and genetically engineered therapeutic applications of serumalbumin.
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Mathematical theory of complex ligand-binding systems at equilibrium: Some methods for parameter fitting
TL;DR: The equilibrium composition of a multicomponent crossreactive ligand-binding system is characterized by solving the mass action equations and several results in simulation and parameter fitting for radioimmunoassay, ion-protein reactions, etc., are derived from these equations.