Journal ArticleDOI
Intron/exon structure of the chicken pyruvate kinase gene
Reads0
Chats0
TLDR
The chicken pyruvate kinase gene is interrupted by at least ten introns, including nine introns within the coding region, which suggests that introns were not inserted into a previously uninterrupted coding sequence, but instead are products of the evolution of the first pyruVate kin enzyme gene.About:
This article is published in Cell.The article was published on 1985-01-01. It has received 125 citations till now. The article focuses on the topics: Exon & Pyruvate kinase.read more
Citations
More filters
Journal ArticleDOI
Three-dimensional structure of calmodulin
Y. Sudhakar Babu,John S. Sack,John S. Sack,Trevor J. Greenhough,Charles E. Bugg,Anthony R. Means,William J. Cook +6 more
TL;DR: The three-dimensional structure of calmodulin has been determined crystallographically at 3.0 Å resolution and it is shown that each lobe binds two calcium ions through helix–loop–helix domains similar to those of other calcium-binding proteins.
Journal ArticleDOI
The M1- and M2-type isozymes of rat pyruvate kinase are produced from the same gene by alternative RNA splicing.
TL;DR: It is concluded that the M1- and M2-type isozymes of pyruvate kinase are produced from the same gene probably by alternative RNA splicing.
Journal ArticleDOI
The molecular biology of intermediate filaments.
TL;DR: The field of IF has now matured to the point where it is being explored by the various approaches generically termed “molecular biology,” with growing evidence that the authors' invertebrate passengers and all of their relatives also contain most of these subclasses and sequence Types.
Book ChapterDOI
Molecular genetics of Na,K-ATPase.
TL;DR: The complex structural and functional features of this protein indicate that extensive research is necessary before a clear understanding of the molecular basis of active cation transport is achieved, and the proposed model and functional hypotheses should be considered judiciously.
References
More filters
Journal ArticleDOI
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
TL;DR: This paper describes a method of transferring fragments of DNA from agarose gels to cellulose nitrate filters that can be hybridized to radioactive RNA and hybrids detected by radioautography or fluorography.
Book ChapterDOI
Sequencing end-labeled DNA with base-specific chemical cleavages.
Allan M. Maxam,Walter Gilbert +1 more
TL;DR: The chapter presents techniques for producing discrete DNA fragments, end-labeling DNA, segregating end- labeled fragments, extracting DNA from gels, and the protocols for partially cleaving it at specific bases using the chemical reactions.
Journal ArticleDOI
Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I
TL;DR: Labeled DNAs (and restriction endonuclease fragments derived from them) are useful probes for detecting rare homologous sequences by in situ hybridization and reassociation kinetic analysis.
Journal ArticleDOI
The Protein Data Bank: a computer-based archival file for macromolecular structures.
Frances C. Bernstein,Thomas F. Koetzle,Graheme J. B. Williams,Edgar F. Meyer,Michael D. Brice,John R. Rodgers,O. Kennard,Takehiko Shimanouchi,Mitsuo Tasumi +8 more
TL;DR: The Protein Data Bank is a computer-based archival file for macromolecular structures that stores in a uniform format atomic co-ordinates and partial bond connectivities, as derived from crystallographic studies.
Journal ArticleDOI
The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.
Jeffrey Vieira,Joachim Messing +1 more
TL;DR: A series of plasmid vectors containing the multiple cloning site (MCS7) of M13mp7 has been constructed and a kanamycin-resistance marker has been inserted into the center of the symmetrical MCS7 to yield a restriction-site-mobilizing element (RSM).