Investigation on cell proliferation with a new antibody against thymidine kinase 1.
Reads0
Chats0
TLDR
A polyclonal anti‐TK1 antibody against a synthetic peptide from the C‐terminus of human TK1 is developed and demonstrated, demonstrating the exclusive location of TK 1 in the cytoplasm of cells.Abstract:
The cytosolic thymidine kinase 1 (TK1) is one of the enzymes involved in DNA replication. Based on biochemical studies, TK1 is activated at late G1 of cell cycle, and its activity correlates with the cell proliferation. We have developed a polyclonal anti‐TK1 antibody against a synthetic peptide from the C‐terminus of human TK1. Using this antibody, here we demonstrate the exclusive location of TK1 in the cytoplasm of cells. Cell cycle dependent TK1 expression was studied by simultaneous fluorescence staining for TK1 and bromodeoxyuridine, by using elutriated cells, and by quantitation of the amount TK1 in relation to the cellular DNA content. TK1, which was strongly expressed in the cells in S+G2 period, raised at late G1 and decreased during mitosis. The amount of TK1 increased three folds from late G1 to G2. TK1 positive cells were demonstrated in areas of proliferation activity of various normal and malignant tissues. The new anti‐TK1 antibody works in archival specimens and is a specific marker of cell proliferation.read more
Citations
More filters
Patent
Exposed proliferation-related peptides, ligands and methods employing the same
TL;DR: In this paper, a novel thymidine kinase (TK1) derived peptide consisting of the amino acid sequence of SEQ ID NO:1 or SEQID NO:2 is employed to obtain ligands having specificity to the peptide.
Book ChapterDOI
22 - Immunohistochemical Expression of Cytosolic Thymidine Kinase in Patients with Breast Carcinoma
Book ChapterDOI
Types of Circulating Tumor Markers and Their Clinical Applications
TL;DR: It was during the 1950s and 1960s when it was realized that the concentration of many molecules circulating in the blood reflected tumor cell activity, which allowed for the adjustment of therapeutic regiment from time to time.
Dissertation
Validation of PET imaging for non-invasive characterization of head and neck tumors.
TL;DR: The current role and future perspectives of PET for selection and delineation of radiotherapy target volumes and for biological tum or characterization in this tumor entity are described.
References
More filters
Journal ArticleDOI
p53 mutations in human cancers
TL;DR: The p53 mutational spectrum differs among cancers of the colon, lung, esophagus, breast, liver, brain, reticuloendothelial tissues, and hemopoietic tissues as mentioned in this paper.
Journal ArticleDOI
Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation
TL;DR: A first series of immunostainings of tumour biopsies indicated that Ki‐67 may be a potent tool for easy and quick evaluation of the proportion of proliferating cells in a tumour.
Journal ArticleDOI
p53 mutations in colorectal cancer.
Nanda R. Rodrigues,Andrew Rowan,M.E.F. Smith,I.B. Kerr,Walter F. Bodmer,Julian Gannon,David P. Lane +6 more
TL;DR: It is concluded that overexpression of p53 is synonymous with mutation, but some mutations would not be detected by a simple immunohistochemical analysis.
Journal ArticleDOI
Review: assessment of cell proliferation in histological material.
P A Hall,D A Levison +1 more
TL;DR: This brief overview will illustrate current ideas about cellular proliferation and its regulation and the advantages and disadvantages of the better known methods for assessing cellular proliferation in histopathological material.
Journal ArticleDOI
Regulation of human thymidine kinase during the cell cycle
James L. Sherley,Thomas J. Kelly +1 more
TL;DR: Two different post-transcriptional mechanisms largely account for the periodic behavior of the enzyme activity during the cell cycle, indicating that the efficiency of translation of thymidine kinase mRNA increases as cells begin DNA replication.