Investigation on cell proliferation with a new antibody against thymidine kinase 1.
TLDR
A polyclonal anti‐TK1 antibody against a synthetic peptide from the C‐terminus of human TK1 is developed and demonstrated, demonstrating the exclusive location of TK 1 in the cytoplasm of cells.Abstract:
The cytosolic thymidine kinase 1 (TK1) is one of the enzymes involved in DNA replication. Based on biochemical studies, TK1 is activated at late G1 of cell cycle, and its activity correlates with the cell proliferation. We have developed a polyclonal anti‐TK1 antibody against a synthetic peptide from the C‐terminus of human TK1. Using this antibody, here we demonstrate the exclusive location of TK1 in the cytoplasm of cells. Cell cycle dependent TK1 expression was studied by simultaneous fluorescence staining for TK1 and bromodeoxyuridine, by using elutriated cells, and by quantitation of the amount TK1 in relation to the cellular DNA content. TK1, which was strongly expressed in the cells in S+G2 period, raised at late G1 and decreased during mitosis. The amount of TK1 increased three folds from late G1 to G2. TK1 positive cells were demonstrated in areas of proliferation activity of various normal and malignant tissues. The new anti‐TK1 antibody works in archival specimens and is a specific marker of cell proliferation.read more
Citations
More filters
Journal ArticleDOI
A New Sandwich ELISA for Quantification of Thymidine Kinase 1 Protein Levels in Sera from Dogs with Different Malignancies Can Aid in Disease Management
TL;DR: The development of a specific sandwich TK1-ELISA has sufficient sensitivity and specificity for routine clinical use in veterinary oncology and is closely associated with clinical outcome in dogs with lymphoma.
Journal ArticleDOI
Assessment of early changes in 3H-fluorothymidine uptake after treatment with gefitinib in human tumor xenograft in comparison with Ki-67 and phospho-EGFR expression
Songji Zhao,Yuji Kuge,Yan Zhao,Satoshi Takeuchi,Kenji Hirata,Toshiki Takei,Tohru Shiga,Hirotoshi Dosaka-Akita,Nagara Tamaki +8 more
TL;DR: 3H-FLT uptake levels significantly decreased after the treatment with two different doses of gefitinib before a significant change in tumor size was observed in an animal model of a human epidermoid cancer.
Journal ArticleDOI
Thymidine kinase-1/CD31 double immunostaining for identifying activated tumor vessels
Shu Okamura,T. Osaki,K. Nishimura,Hiroyuki Ohsaki,Michiko Shintani,Hiroshi Matsuoka,Kotaro Maeda,Kazuya Shiogama,Tomoo Itoh,Shingo Kamoshida +9 more
TL;DR: It is indicated that double immunostaining for TK1/CD31 can detect activated tumor vessels more accurately than staining for Ki67/ CD31 and potentially could identify tumors that will respond to anti-angiogenic therapy.
Journal ArticleDOI
Exposed proliferation antigen 210 (XPA-210) in renal cell carcinoma (RCC) and oncocytoma: clinical utility and biological implications.
Stephan Kruck,Joerg Hennenlotter,Ulrich Vogel,David Schilling,Georgios Gakis,Joachim Hevler,Ursula Kuehs,Arnulf Stenzl,Christian Schwentner +8 more
TL;DR: The largest ever study of its kind has been conducted on the role of language and culture in the development of Alzheimer's disease and its effects on physical and mental health.
Thymidine Kinase 1: Diagnostic and Prognostic Significance in Malignancy
TL;DR: TK1 has promising diagnostic potential in a variety of malignancies and is elevated in early cancer development, according to this work, which proposes one sensitive and specific method of detection, namely a TK1 ELISA.
References
More filters
Journal ArticleDOI
p53 mutations in human cancers
TL;DR: The p53 mutational spectrum differs among cancers of the colon, lung, esophagus, breast, liver, brain, reticuloendothelial tissues, and hemopoietic tissues as mentioned in this paper.
Journal ArticleDOI
Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation
TL;DR: A first series of immunostainings of tumour biopsies indicated that Ki‐67 may be a potent tool for easy and quick evaluation of the proportion of proliferating cells in a tumour.
Journal ArticleDOI
p53 mutations in colorectal cancer.
Nanda R. Rodrigues,Andrew Rowan,M.E.F. Smith,I.B. Kerr,Walter F. Bodmer,Julian Gannon,David P. Lane +6 more
TL;DR: It is concluded that overexpression of p53 is synonymous with mutation, but some mutations would not be detected by a simple immunohistochemical analysis.
Journal ArticleDOI
Review: assessment of cell proliferation in histological material.
P A Hall,D A Levison +1 more
TL;DR: This brief overview will illustrate current ideas about cellular proliferation and its regulation and the advantages and disadvantages of the better known methods for assessing cellular proliferation in histopathological material.
Journal ArticleDOI
Regulation of human thymidine kinase during the cell cycle
James L. Sherley,Thomas J. Kelly +1 more
TL;DR: Two different post-transcriptional mechanisms largely account for the periodic behavior of the enzyme activity during the cell cycle, indicating that the efficiency of translation of thymidine kinase mRNA increases as cells begin DNA replication.