Journal ArticleDOI
Isolation and partial characterization of components of Prunus avium L. styles, including an antigenic glycoprotein associated with a self-incompatibility genotype.
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TLDR
Several components of buffer extracts of Prunus avium L. styles have been isolated and partially characterized: the major component is a glycoprotein and a “sticky” uronic-acid-containing component and an arabinogalactan are also present.Abstract:
Several components of buffer extracts of Prunus avium L. styles (cv. Lambert, S3S4) have been isolated and partially characterized: the major component is a glycoprotein (molecular weight approx. 90,000; 95% protein, 5.4% carbohydrate). A “sticky” uronic-acid-containing component and an arabinogalactan are also present. Two minor components are an antigenic glycoprotein associated with the self-incompatibility genotype (Antigen S) and a component found in styles of all Prunus species (Antigen P). The isolated glycoproteins have a substantial carbohydrate content (Antigen P 17.2%; Antigen S 16.3%), and have apparent molecular weights of 32,000 (Antigen P) and 37,000–39,000 (Antigen S). They are antigenically quite distinct. Material corresponding to Antigen S is secreted into the medium of suspension-cultured callus cells raised from both leaf and stem of P. avium.read more
Citations
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Journal ArticleDOI
Cloning of cDNA for a stylar glycoprotein associated with expression of self-incompatibility in Nicotiana alata
Marilyn A. Anderson,Edwina C. Cornish,Shaio-Lim Mau,E. G. Williams,R. Hoggart,Atkinson Angela Hilary,Ingrid Bonig,Boris Grego,Richard J. Simpson,P. J. Roche,John D. Haley,Jenny Penschow,H. D. Niall,G. W. Tregear,John P. Coghlan,Robert J. Crawford,Adrienne E. Clarke +16 more
TL;DR: A complementary DNA clone encoding a style protein of Nicotiana alata which segregates with the S2 allele has now been sequenced and shows that the S-allele-associated style components in different genotypes of N. alata and in Lycopersicon peruvianum are homologous.
Book ChapterDOI
Pollen-Pistil Interactions
TL;DR: The pollination process is susceptible to modification to permit the introduction of desirable genes, for example, for disease resistance, from wild species into crop plants in order to maximize fertilization and hence crop production.
Journal ArticleDOI
cDNA cloning and molecular analysis of two self-incompatibility alleles from apple
TL;DR: The structural features of S-RNase deduced from a comparison of their sequences are discussed, and eight invariable cysteine residues, conserved regions around two histidines thought to play a role in RNA catalysis, and a number of other distinct residues are conserved between the apple S- RNases and similar proteins in the family Solanaceae.
Journal ArticleDOI
Primary structural features of the self-incompatibility protein in solanaceae
TL;DR: A sequence comparison of 12 S-allele-associated proteins from three solanaceous species with gametophytic self-incompatibility is presented, which confirms the previously recognized similarity between 3 more closely related N. alata S-proteins and these ribonucleases and argues strongly for the functional importance of the ribonuclease activity of the S-protein in self- incompatibility.
Journal ArticleDOI
Callose and determination of pistil viability and incompatibility.
C. Dumas,Robert Bruce Knox +1 more
TL;DR: The role of callose in pollen-stigma interactions has many analogies with host-parasite interactions, and a model is proposed based on relationships between callose, boron and inhibitor (phytoalexin-like) synthesis.
References
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Journal ArticleDOI
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