Journal ArticleDOI
JC‐1, but not DiOC6(3) or rhodamine 123, is a reliable fluorescent probe to assess ΔΨ changes in intact cells: implications for studies on mitochondrial functionality during apoptosis
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Data indicate that JC‐1 is a reliable probe for analyzing ΔΨ changes with flow cytometry, while the others show a lower sensitivity (R123), or a non‐coherent behaviour, due to a high sensitivity to changes in plasmamembrane potential [DiOC6(3].About:
This article is published in FEBS Letters.The article was published on 1997-07-07. It has received 1003 citations till now. The article focuses on the topics: Rhodamine 123.read more
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The mitochondrial membrane potential (deltapsi(m)) in apoptosis; an update.
TL;DR: However, there are emerging data suggesting that, depending on the model of apoptosis, the loss of deltapsi(m) may not be an early requirement for apoptosis but on the contrary may be a consequence of the apoptotic-signaling pathway as mentioned in this paper.
Journal ArticleDOI
Mitochondria and Neuronal Survival
TL;DR: The functional bioenergetics of isolated mitochondria are reviewed, with emphasis on the chemiosmotic proton circuit and the application (and occasional misapplication) of these principles to intact neurons.
Journal ArticleDOI
Mitochondrial membrane potential.
Ljubava D. Zorova,Vasily A. Popkov,Egor Y. Plotnikov,Denis N. Silachev,Irina B. Pevzner,S. S. Jankauskas,V. A. Babenko,S. D. Zorov,Anastasia V. Balakireva,Magdalena Juhaszova,Steven J. Sollott,Dmitry B. Zorov,Dmitry B. Zorov +12 more
TL;DR: Additional potential mechanisms for which ΔΨm is essential for maintenance of cellular health and viability are proposed and recommendations how to accurately measure ΔΩm in a cell are provided and potential sources of artifacts are discussed.
Journal ArticleDOI
Mitochondrial membrane potential probes and the proton gradient: a practical usage guide.
TL;DR: This review will help illustrate both the strengths and potential pitfalls of common mitochondrial membrane potential dyes, and highlight best-usage approaches for their efficacious application in life sciences research.
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Mitochondria-Targeted Triphenylphosphonium-Based Compounds: Syntheses, Mechanisms of Action, and Therapeutic and Diagnostic Applications
Jacek Zielonka,Joy Joseph,Adam Sikora,Micael Hardy,Olivier Ouari,Jeannette Vasquez-Vivar,Gang Cheng,Marcos Lopez,Balaraman Kalyanaraman +8 more
TL;DR: The physicochemical basis for mitochondrial accumulation of lipophilic cations, synthetic chemistry strategies to target compounds to mitochondria, mitochondrial probes, and sensors, and examples of mitochondrial targeting of bioactive compounds are described.
References
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Journal ArticleDOI
Prevention of Apoptosis by Bcl-2: Release of Cytochrome c from Mitochondria Blocked
Jie Yang,Xuesong Liu,Xuesong Liu,Kapil N. Bhalla,Caryn Naekyung Kim,Ana Maria Ibrado,Jiyang Cai,Tsung I. Peng,Dean P. Jones,Xiaodong Wang,Xiaodong Wang +10 more
TL;DR: One possible role of Bcl-2 in prevention of apoptosis is to block cytochrome c release from mitochondria, which is normally located in the mitochondrial intermembrane space.
Journal ArticleDOI
The Release of Cytochrome c from Mitochondria: A Primary Site for Bcl-2 Regulation of Apoptosis
TL;DR: In a cell-free apoptosis system, mitochondria spontaneously released cytochrome c, which activated DEVD-specific caspases, leading to fodrin cleavage and apoptotic nuclear morphology, and Bcl-2 acts to inhibit cy tochrome c translocation, thereby blocking caspase activation and the apoptotic process.
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Sequential reduction of mitochondrial transmembrane potential and generation of reactive oxygen species in early programmed cell death.
Naoufal Zamzami,Philippe Marchetti,Maria Castedo,Didier Decaudin,Antonio Macho,Tamara Hirsch,Santos A. Susin,Patrice X. Petit,Bernard Mignotte,Guido Kroemer +9 more
TL;DR: Assessment of mitochondrial ROS generation provides an accurate picture of PCD-mediated lymphocyte depletion and indicates alterations of mitochondrial function constitute an important feature of early PCD.
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Localization of mitochondria in living cells with rhodamine 123
TL;DR: With the use of rhodamine 123, it is possible to detect alterations in mitochondrial distribution following transformation by Rous sarcoma virus and changes in the shape and organization of mitochondria induced by colchicine treatment.
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Intracellular heterogeneity in mitochondrial membrane potentials revealed by a J-aggregate-forming lipophilic cation JC-1.
Stephen T. Smiley,Martin Reers,Cristina Mottola-Hartshorn,Mei Lin,A. P. Chen,Thomas W. Smith,Glenn Steele,Lan Bo Chen +7 more
TL;DR: It is found that membrane potentials across mitochondria in a living cell can be heterogeneous, and even within a long contiguous mitochondrion, regional heterogeneity in membranes potentials appears to be possible.