Journal ArticleDOI
Key issues in non-viral gene delivery
Colin W. Pouton,Len Seymour +1 more
TLDR
Present knowledge of the biodistribution and cellular interactions of gene delivery systems are summarized and how improvements in gene delivery will be accomplished in the future are considered.About:
This article is published in Advanced Drug Delivery Reviews.The article was published on 1998-10-05. It has received 455 citations till now. The article focuses on the topics: Gene delivery.read more
Citations
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Journal ArticleDOI
Nonviral Vectors for Gene Delivery
TL;DR: Two nonviral gene delivery systems using either biodegradable poly(D,Llactide-co-glycolide) (PLG) nanoparticles or cell penetrating peptide (CPP) complexes have been designed and studied using A549 human lung epithelial cells.
Journal ArticleDOI
Synthetic DNA delivery systems.
Dan Luo,Saltzman Wm +1 more
TL;DR: The ability to safely and efficiently transfer foreign DNA into cells is a fundamental goal in biotechnology and rapid advances have recently been made in understanding of mechanisms for DNA stability and transport within cells.
Journal ArticleDOI
Polymer microspheres for controlled drug release
S. Freiberg,X. X. Zhu +1 more
TL;DR: This review presents the methods used in the preparation of microspheres from monomers or from linear polymers and discusses the physio-chemical properties that affect the formation, structure, and morphology of the spheres.
Journal ArticleDOI
Chitosan-DNA nanoparticles as gene carriers : synthesis, characterization and transfection efficiency
Hai-Quan Mao,Krishnendu Roy,Vu L. Troung-Le,Kevin A. Janes,Kevin Y. Lin,Yan Wang,J. Thomas August,Kam W. Leong +7 more
TL;DR: The chitosan-DNA nanoparticles could partially protect the encapsulated plasmid DNA from nuclease degradation as shown by electrophoretic mobility analysis and three different schemes to conjugate transferrin or KNOB protein to the nanoparticle surface were developed.
Journal ArticleDOI
Inorganic nanoparticles as carriers for efficient cellular delivery
TL;DR: The fundamental understanding of properties of inorganic nanoparticles in relation to cellular delivery efficiency as the most paramount issue will be highlighted and some key issues in efficient cellular delivery using in organic nanoparticles are highlighted.
References
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Journal ArticleDOI
Direct gene transfer into mouse muscle in vivo.
Jon A. Wolff,Robert W. Malone,Phillip Williams,Wang Chong,Gyula Acsadi,Agnes Jani,Philip L. Felgner +6 more
TL;DR: RNA and DNA expression vectors containing genes for chloramphenicol acetyltransferase, luciferase, and beta-galactosidase were separately injected into mouse skeletal muscle in vivo and expression was comparable to that obtained from fibroblasts transfected in vitro under optimal conditions.
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Genetic immunization is a simple method for eliciting an immune response.
TL;DR: It is reported that an immune response can be elicited by introducing the gene encoding a protein directly into the skin of mice by using a hand-held form of the biolistic system.
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Cellular and Molecular Barriers to Gene Transfer by a Cationic Lipid
TL;DR: The results indicate that endocytosis was the major mechanism of entry in cationic lipid-mediated gene transfer and suggest that attention to specific steps in the cellular process may further improve the efficiency of transfection and increase its use in a number of applications.
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Mechanism of DNA release from cationic liposome/DNA complexes used in cell transfection.
Yuhong Xu,Francis C. Szoka +1 more
TL;DR: It is proposed that after the cationic lipid/DNA complex is internalized into cells by endocytosis it destabilization induces flip-flop of anionic lipids from the cytoplasmic-facing monolayer, which laterally diffuse into the complex and form a charge neutral ion pair with the cationsic lipids.
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DNA-based immunization by in vivo transfection of dendritic cells
TL;DR: It is shown that cutaneous genetic immunization with naked DNA results in potent, antigen–specific, cytotoxic T lymphocyte–mediated protective tumor immunity and the feasibility of genetically engineering dendritic cells in vivo.