Journal ArticleDOI
Lipid- and polymer-based nanoparticle systems for the delivery of CRISPR/Cas9
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TLDR
In this article, the authors discuss and critic nanoparticle delivery strategies, focusing on the use of lipid-based and polymeric-based matrices, for the delivery of CRISPR therapeutics.About:
This article is published in Journal of Drug Delivery Science and Technology.The article was published on 2021-10-01. It has received 20 citations till now. The article focuses on the topics: CRISPR & Genome editing.read more
Citations
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Journal ArticleDOI
Mechanism and Applications of CRISPR/Cas-9-Mediated Genome Editing
Misganaw Asmamaw,Belay Zawdie +1 more
TL;DR: The mechanism of CRISPR/Cas-9 genome editing contains three steps, recognition, cleavage, and repair as discussed by the authors, which is the most effective, efficient, and accurate method of genome editing in all living cells and utilized in many applied disciplines.
Journal ArticleDOI
Lipid-based nanocarrier mediated CRISPR/Cas9 delivery for cancer therapy
TL;DR: The design and genome editing application in cancer therapy has been discussed along with the future concern and prospects of lipid nanoparticle based CRISPR/Cas9 has been focused toward the end.
Journal ArticleDOI
Genome editing via non-viral delivery platforms: current progress in personalized cancer therapy
TL;DR: In this paper , the important attributes and recent progress of genome editing tools are discussed, and the laboratory and clinical investigations that seek for the possibility of combining non-viral delivery systems with GETs for the treatment of cancer are assessed in the scope of personalized therapy.
Journal ArticleDOI
Progress of delivery methods for CRISPR-Cas9
TL;DR: The development of delivery methods is highly likely to take the application of CRISPR-Cas9 technology to another level, and the complex nuclease and protease environment is a tremendous challenge for in vivo clinical applications.
Journal ArticleDOI
Genome editing via non-viral delivery platforms: current progress in personalized cancer therapy
TL;DR: In this paper , the important attributes and recent progress of genome editing tools are discussed, and the laboratory and clinical investigations that seek for the possibility of combining non-viral delivery systems with GETs for the treatment of cancer are assessed in the scope of personalized therapy.
References
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Journal ArticleDOI
Multiplex Genome Engineering Using CRISPR/Cas Systems
Le Cong,Le Cong,F. Ann Ran,F. Ann Ran,David M. Cox,David M. Cox,Shuailiang Lin,Shuailiang Lin,Robert P. J. Barretto,Naomi Habib,Patrick D. Hsu,Patrick D. Hsu,Xuebing Wu,Wenyan Jiang,Luciano A. Marraffini,Feng Zhang +15 more
TL;DR: The type II prokaryotic CRISPR (clustered regularly interspaced short palindromic repeats)/Cas adaptive immune system has been shown to facilitate RNA-guided site-specific DNA cleavage as discussed by the authors.
Journal ArticleDOI
The new frontier of genome engineering with CRISPR-Cas9
TL;DR: The power of the CRISPR-Cas9 technology to systematically analyze gene functions in mammalian cells, study genomic rearrangements and the progression of cancers or other diseases, and potentially correct genetic mutations responsible for inherited disorders is illustrated.
Journal ArticleDOI
Principles of nanoparticle design for overcoming biological barriers to drug delivery
TL;DR: By successively addressing each of the biological barriers that a particle encounters upon intravenous administration, innovative design features can be rationally incorporated that will create a new generation of nanotherapeutics, realizing a paradigmatic shift in nanoparticle-based drug delivery.
Journal ArticleDOI
CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes
Luke A. Gilbert,Matthew H. Larson,Leonardo Morsut,Zairan Liu,Gloria A. Brar,Sandra Elizabeth Torres,Noam Stern-Ginossar,Onn Brandman,Evan H. Whitehead,Jennifer A. Doudna,Wendell A. Lim,Jonathan S. Weissman,Lei S. Qi +12 more
TL;DR: The results establish that the CRISPR system can be used as a modular and flexible DNA-binding platform for the recruitment of proteins to a target DNA sequence, revealing the potential of CRISpri as a general tool for the precise regulation of gene expression in eukaryotic cells.
Journal ArticleDOI
Designing hydrogels for controlled drug delivery.
Jianyu Li,David J. Mooney +1 more
TL;DR: This Review discusses how different mechanisms interact and can be integrated to exert fine control in time and space over the drug presentation, and collects experimental release data from the literature and presents quantitative comparisons between different systems to provide guidelines for the rational design of hydrogel delivery systems.
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Non-viral delivery systems for CRISPR/Cas9-based genome editing: Challenges and opportunities
Ling Li,Shuo Hu,Xiaoyuan Chen +2 more