Liquid-liquid phase separation in human health and diseases.
Bin Wang,Bin Wang,Zhang Lei,Tong Dai,Ziran Qin,Ziran Qin,Huasong Lu,Long Zhang,Fangfang Zhou +8 more
TLDR
A review of liquid-liquid phase separation (LLPS) in eukaryotic cells can be found in this paper, where the authors describe the current understanding of LLPS and summarize its physiological functions.Abstract:
Emerging evidence suggests that liquid-liquid phase separation (LLPS) represents a vital and ubiquitous phenomenon underlying the formation of membraneless organelles in eukaryotic cells (also known as biomolecular condensates or droplets). Recent studies have revealed evidences that indicate that LLPS plays a vital role in human health and diseases. In this review, we describe our current understanding of LLPS and summarize its physiological functions. We further describe the role of LLPS in the development of human diseases. Additionally, we review the recently developed methods for studying LLPS. Although LLPS research is in its infancy-but is fast-growing-it is clear that LLPS plays an essential role in the development of pathophysiological conditions. This highlights the need for an overview of the recent advances in the field to translate our current knowledge regarding LLPS into therapeutic discoveries.read more
Citations
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Liquid–liquid phase separation in tumor biology
TL;DR: In this article , the authors comprehensively summarize the detailed mechanisms of biomolecular condensate formation and biophysical function and review the recent major advances toward elucidating the multiple mechanisms involved in cancer cell pathology driven by aberrant liquid-liquid phase separation (LLPS).
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ALS-linked KIF5A ΔExon27 mutant causes neuronal toxicity through gain of function
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Improved Predictions of Phase Behaviour of Intrinsically Disordered Proteins by Tuning the Interaction Range
TL;DR: CALVADOS 2 is presented, a coarse-grained model of IDPs that accurately predicts conformational properties and propensity to undergo PS for diverse sequences and solution conditions and which accurately predicts chain compaction and PS propensity for sequences of diverse length and charge patterning.
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Effects of pH alterations on stress- and aging-induced protein phase separation
TL;DR: In this article , the authors summarized stress-, aging-, and cancer-associated pH changes together with the mechanisms by which cells regulate cytosolic pH homeostasis, along with the functional roles of intracellular pH fluctuation in the regulation of liquid-liquid phase separation.
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