LPS-induced autophagy is mediated by oxidative signaling in cardiomyocytes and is associated with cytoprotection.
Hua Yuan,Cynthia N. Perry,Chengqun Huang,Eri Iwai-Kanai,Raquel S. Carreira,Christopher C. Glembotski,Roberta A. Gottlieb +6 more
TLDR
The notion that autophagy is a cytoprotective response to LPS-induced cardiomyocyte injury is supported; additional studies are needed to determine the therapeutic implications.Abstract:
Bacterial endotoxin lipopolysaccharide (LPS) is responsible for the multiorgan dysfunction that characterizes septic shock and is causal in the myocardial depression that is a common feature of end...read more
Citations
More filters
Journal ArticleDOI
Defective hepatic autophagy in obesity promotes ER stress and causes insulin resistance.
TL;DR: The data demonstrate that autophagy is an important regulator of organelle function and insulin signaling and that loss of autophile is a critical component of defective insulin action seen in obesity.
Journal ArticleDOI
Reactive Oxygen Species in Metabolic and Inflammatory Signaling.
TL;DR: The role of ROS in the regulation metabolic/inflammatory diseases including atherosclerosis, diabetes mellitus, and stroke is highlighted and the balance ROS signaling plays in both physiology and pathophysiology is understood.
Journal ArticleDOI
Autophagy, Immunity, and Microbial Adaptations
Vojo Deretic,Beth Levine +1 more
TL;DR: The ability of eukaryotic pathogens to deploy their own autophagic machinery may also contribute to microbial pathogenesis, and a complex interplay between Autophagy and microbial adaptations against autophagy governs the net outcome of host-microbe encounters.
Journal ArticleDOI
Apoptosis and glutathione: beyond an antioxidant
Rodrigo Franco,John A. Cidlowski +1 more
TL;DR: This work reformulates emerging paradigms of apoptotic cell death into current understanding of cell death mechanisms and suggests that GSH depletion and post-translational modifications of proteins through glutathionylation are critical regulators of apoptosis.
Journal ArticleDOI
A lysosome-targetable and two-photon fluorescent probe for monitoring endogenous and exogenous nitric oxide in living cells.
TL;DR: With the aid of Lyso-NINO, the first capture of NO within lysosomes of macrophage cells has been achieved using both two-photon fluorescence microscopy and flow cytometry.
References
More filters
Journal ArticleDOI
Reactive oxygen intermediates as apparently widely used messengers in the activation of the NF-kappa B transcription factor and HIV-1.
TL;DR: It is shown that micromolar concentrations of H2O2 can induce the expression and replication of HIV‐1 in a human T cell line and suggests that diverse agents thought to activate NF‐kappa B by distinct intracellular pathways might all act through a common mechanism involving the synthesis of ROI.
Journal ArticleDOI
Fluorescence probe measurement of the intralysosomal pH in living cells and the perturbation of pH by various agents
Shoji Ohkuma,Brian Poole +1 more
TL;DR: The results provide evidence for the existence of an active proton accumulation mechanism in the lysosomal membrane and support the theory of lysOSomal accumulation of weak bases by proton trapping.
Journal ArticleDOI
Reactive oxygen species are essential for autophagy and specifically regulate the activity of Atg4
TL;DR: The role of reactive oxygen species (ROS) as signaling molecules in starvation‐induced autophagy is described and a cysteine residue located near the HsAtg4 catalytic site is specified as a critical for this regulation.
Journal ArticleDOI
HL-1 cells: A cardiac muscle cell line that contracts and retains phenotypic characteristics of the adult cardiomyocyte
William C. Claycomb,Nicholas A. Lanson,Beverly S. Stallworth,Daniel B. Egeland,Joseph B. Delcarpio,Anthony Bahinski,Nicholas J. Izzo +6 more
TL;DR: In this paper, the authors derived a cardiac muscle cell line, designated HL-1, from the AT-1 mouse atrial cardiomyocyte tumor lineage, which can be serially passaged, yet they maintain the ability to contract and retain differentiated cardiac morphological, biochemical, and electrophysiological properties.
Journal ArticleDOI
Role of Bcl-2 family proteins in a non-apoptotic programmed cell death dependent on autophagy genes.
Shigeomi Shimizu,Toku Kanaseki,Noboru Mizushima,Noboru Mizushima,Noboru Mizushima,Takeshi Mizuta,Satoko Arakawa-Kobayashi,Craig B. Thompson,Yoshihide Tsujimoto +8 more
TL;DR: Results indicate that the Bcl-2 family of proteins not only regulates apoptosis, but also controls non-apoptotic programmed cell death that depends on the autophagy genes.