Mapping the extended substrate binding site of cathepsin G and human leukocyte elastase. Studies with peptide substrates related to the alpha 1-protease inhibitor reactive site.
TLDR
Two tetrapeptide 4-nitroanilide substrates related to the reactive site of the plasma (~1’ protease inhibitor) were studied and indicate that oxidation of the PI Met of YePI is capable to destroying its reactivity toward most serine proteases.About:
This article is published in Journal of Biological Chemistry.The article was published on 1979-05-25 and is currently open access. It has received 610 citations till now. The article focuses on the topics: Protease inhibitor (biology) & Cathepsin L1.read more
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Irreversible inhibitors of serine, cysteine, and threonine proteases.
TL;DR: This paper presents a meta-review of the literature on Vinyl Sulfones, Michael Acceptors, and Heterocyclic Inhibitors dating back to the 1970s, which revealed a wide diversity of opinions about the properties of these substances and their role in the human immune system.
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Kinetics of association of serine proteinases with native and oxidized alpha-1-proteinase inhibitor and alpha-1-antichymotrypsin.
K. Beatty,J. Bieth,James Travis +2 more
TL;DR: Data support previous results which indicated that oxidation of human alpha-1-proteinase inhibitor in vivo could reduce the effectiveness of this inhibitor in controlling proteolysis in the lung, in particular, in the development of pulmonary emphysema.
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Neutrophil Elastase, Proteinase 3, and Cathepsin G as Therapeutic Targets in Human Diseases
TL;DR: The physicochemical functions of hematopoietic serine proteases are described, toward a goal of better delineating their role in human diseases and identifying new therapeutic strategies based on the modulation of their bioavailability and activity.
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Structure and variation of human α 1 -antitrypsin
Robin W. Carrell,Jan-Olof Jeppsson,Carl-Bertil Laurell,Stephen O. Brennan,Maurice C. Owen,Lloyd Vaughan,D. Ross Boswell +6 more
TL;DR: The sequence of α1–antitrypsin is in keeping with its role as a tissue scavenger of leukocyte elastase and helps explain why cigarette smoking greatly accelerates the onset and severity of this degenerative process to give the disease emphysema.
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Vascular cell adhesion molecule-1 (CD106) is cleaved by neutrophil proteases in the bone marrow following hematopoietic progenitor cell mobilization by granulocyte colony-stimulating factor
TL;DR: It is made that an essential step contributing to the mobilization of HPCs is the proteolytic cleavage of VCAM-1 expressed by BM stromal cells, an event triggered by the degranulation of neutrophils accumulating in the BM in response to the administration of G-CSF.
References
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On the size of the active site in proteases. I. Papain.
Israel Schechter,Arieh Berger +1 more
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The preparation and properties of two new chromogenic substrates of trypsin.
TL;DR: Preliminary studies indicate that benzoyl dl -arginine p -nitroanilide hydrochloride is also hydrolyzed by papain, and that of l -LPA is in a more alkaline region than normally found for trypsin substrates.
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The synthesis and analytical use of a highly sensitive and convenient substrate of elastase.
TL;DR: The synthesis and analytical use of succinyl-( l -alanine) 3 - p -nitroanilide, a new elastase substrate that is virtually not attacked by trypsin or chymotrypsin and does not undergo appreciable spontaneous hydrolysis is described.
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The oxidative inactivation of human alpha-1-proteinase inhibitor. Further evidence for methionine at the reactive center.
David A. Johnson,James Travis +1 more
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Crystal structure of the complex of porcine trypsin with soybean trypsin inhibitor (Kunitz) at 2.6-A resolution.
TL;DR: In the resulting electron density maps the trypsin molecule was easily identifiable, and although some parts of the inhibitor were not clear, a precise interpretation of residues 1'-93' was made, including the con- tact region.
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