Journal ArticleDOI
Mast cell lines produce lymphokines in response to cross-linkage of Fc epsilon RI or to calcium ionophores.
Marshall Plaut,Jacalyn H. Pierce,Cynthia J. Watson,J. Hanley-Hyde,Richard P. Nordan,William E. Paul +5 more
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TLDR
It is shown that cross-linkage of FcεRI on a series of non-transformed murine mast cell lines, or treatment of these cells with calcium ionophores, stimulates increased messenger RNA levels and secretion of a group of lymphokines classically produced by a subset of murine T cell lines (TH2cells).Abstract:
The cross-linkage of high affinity Fc epsilon receptors (Fc epsilon RI) on mast cells and basophils is central to the induction of allergic inflammatory responses. As a result of such cross-linkage, mast cells secrete a variety of preformed biologically active substances, such as histamine, and newly synthesized arachidonic acid metabolites. Here we show that cross-linkage of Fc epsilon RI on a series of nontransformed murine mast cell lines, or treatment of these cells with calcium ionophores, stimulates increased messenger RNA levels and secretion of a group of lymphokines classically produced by a subset of murine T cell lines (TH2 cells). These factors include interleukin-3 (a mast cell growth factor)s interleukin-4 (an IgE 'switch factor'), interleukin-5 (an eosinophil differentiation factor) and interleukin-6 (a factor controlling immunoglobulin secretion). The production of these polypeptide factors by mast cells may have great importance in the induction of allergic and anti-parasite inflammatory responses.read more
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Effect of Rehmannia glutinosa on immediate type allergic reaction
TL;DR: Results indicate that RGAE may be beneficial in the regulation of immediate type allergic reaction and had significant inhibitory effect on anti-DNP IgE-induced tumor necrosis factor-alpha production of RPMC.
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Early and late asthmatic reaction after allergen challenge
TL;DR: It is intriguing that AR is already increased 3 h after allergen challenge, when the FEV, has returned to prechallenge values, thus even preceding the LAR.
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Contribution of mast cells to bacterial clearance and their proliferation during experimental cystitis induced by type 1 fimbriated E. coli
TL;DR: The findings suggest a beneficial role of mast cells in urinary bladder infections in mice, which is likely due to an increase in the mast cell growth-promoting cytokine IL-3 in bacteria-activated mast cells.
Journal Article
Detection of mouse mast cell-associated protease mRNA. Heparinase treatment greatly improves RT-PCR of tissues containing mast cell heparin.
TL;DR: RT-PCR after heparinase treatment appears to represent an extremely sensitive method for detectingmast cell-associated transcripts in tissue specimens, permitting the identification of transcripts for mast cell-specific proteases in the skin of genetically mastcell-deficient WBB6F1-W/WV mice.
Journal ArticleDOI
Linker for Activation of T Cells Integrates Positive and Negative Signaling in Mast Cells
Odile Malbec,Odile Malbec,Marie Malissen,Isabelle Isnardi,Isabelle Isnardi,Renaud Lesourne,Renaud Lesourne,Anne-Marie Mura,Wolf H. Fridman,Bernard Malissen,Marc Daëron,Marc Daëron +11 more
TL;DR: It is found that LAT tyrosines differentially contribute to exocytosis and cytokine secretion and differentially regulate biological responses of mucosal- and serosal-type mast cells.
References
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Journal ArticleDOI
Identification of a common nucleotide sequence in the 3'-untranslated region of mRNA molecules specifying inflammatory mediators
TL;DR: A 33-nucleotide sequence, comprised entirely of A and T residues and located in the 3'-untranslated region, is conserved in toto in the murine and human TNF mRNAs.
Journal ArticleDOI
Production of hybridoma growth factor by human monocytes.
TL;DR: Limiting dilution analysis of the producing cells in combination with size, density and adherence characteristics showed that HGF is produced by monocytes and not by lymphocytes, and there was no need for the monocytes to be stimulated but the cells did require the presence of serum.
Journal ArticleDOI
Two types of mouse helper T cell clone. III. Further differences in lymphokine synthesis between Th1 and Th2 clones revealed by RNA hybridization, functionally monospecific bioassays, and monoclonal antibodies.
TL;DR: Lymphokine synthesis patterns of a panel of 19 T cell clones have been evaluated, using mRNA hybridization methods to examine 11 different mRNAs induced by Con A, and it is shown that secreted protein and mRNA levels correlated well for all cell lines.
Journal Article
B cell stimulatory factor-1 enhances the IgE response of lipopolysaccharide-activated B cells.
TL;DR: Highly purified BSF-1 from a different source, the T lymphoma cell line EL-4, enhanced IgE production to the same extent as TH supernatants, which suggests that B SF-1 is responsible for this increase in IgEProduction.
Journal ArticleDOI
The role of helper T cell products in mouse B cell differentiation and isotype regulation.
Robert L. Coffman,Brian W. P. Seymour,Deborah A. Lebman,Debra D. Hiraki,Judy A. Christiansen,B Shrader,Holly M. Cherwinski,Huub F. J. Savelkoul,Fred D. Finkelman,Martha W. Bond,Tim R. Mosmann +10 more
TL;DR: In this article, the authors summarize the efforts to define the specific helper T cells or lymphokines which transmit regulatory signals to B cells, and to clarify the individual events influenced by these signals.
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