Journal ArticleDOI
Mast cell lines produce lymphokines in response to cross-linkage of Fc epsilon RI or to calcium ionophores.
Marshall Plaut,Jacalyn H. Pierce,Cynthia J. Watson,J. Hanley-Hyde,Richard P. Nordan,William E. Paul +5 more
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TLDR
It is shown that cross-linkage of FcεRI on a series of non-transformed murine mast cell lines, or treatment of these cells with calcium ionophores, stimulates increased messenger RNA levels and secretion of a group of lymphokines classically produced by a subset of murine T cell lines (TH2cells).Abstract:
The cross-linkage of high affinity Fc epsilon receptors (Fc epsilon RI) on mast cells and basophils is central to the induction of allergic inflammatory responses. As a result of such cross-linkage, mast cells secrete a variety of preformed biologically active substances, such as histamine, and newly synthesized arachidonic acid metabolites. Here we show that cross-linkage of Fc epsilon RI on a series of nontransformed murine mast cell lines, or treatment of these cells with calcium ionophores, stimulates increased messenger RNA levels and secretion of a group of lymphokines classically produced by a subset of murine T cell lines (TH2 cells). These factors include interleukin-3 (a mast cell growth factor)s interleukin-4 (an IgE 'switch factor'), interleukin-5 (an eosinophil differentiation factor) and interleukin-6 (a factor controlling immunoglobulin secretion). The production of these polypeptide factors by mast cells may have great importance in the induction of allergic and anti-parasite inflammatory responses.read more
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Journal ArticleDOI
Regulation of interleukin 3 mRNA expression in mast cells occurs at the posttranscriptional level and is mediated by calcium ions
TL;DR: Results indicate that control of expression of the IL-3 gene in mast cells is primarily at the posttranscriptional level and that the Ca2(+)-dependent signal-transduction pathway plays an important role in this process.
Book ChapterDOI
The pathobiology of bronchial asthma.
Jonathan P. Arm,Tak H. Lee +1 more
TL;DR: The severity of clinical asthma correlates with several measurements of the severity of the inflammatory response, suggesting a crucial role for airway inflammation in the pathophysiology of the disease.
Journal Article
SLP-76 deficiency impairs signaling via the high-affinity IgE receptor in mast cells.
Vadim Pivniouk,Thomas R. Martin,Jennifer M. Lu-Kuo,Howard R. Katz,Hans C. Oettgen,Raif S. Geha +5 more
TL;DR: Results suggest that SLP-76 plays an important role in FcepsilonRI-mediated signaling in mast cells and Bone marrow-derived mast cells from SLP76(-/-) mice failed to release beta-hexosaminidase and to secrete IL-6 after FcePSilonRI cross-linking.
Journal ArticleDOI
Growth and Gene Expression Are Predominantly Controlled by Distinct Regions of the Human IL-4 Receptor
John J. Ryan,Lisa J. McReynolds,Achsah D. Keegan,Lu Hai Wang,Evan Garfein,Paul B. Rothman,Keats Nelms,William E. Paul +7 more
TL;DR: The results indicate that growth regulation and gene expression are principally controlled by distinct regions of IL-4R.
Journal ArticleDOI
Mast cell tumor necrosis factor α production is regulated by MEK kinases
Tamotsu Ishizuka,Naohiro Terada,Pär Gerwins,Eckard Hamelmann,Akihiro Oshiba,Gary R. Fanger,Gary L. Johnson,Erwin W. Gelfand +7 more
TL;DR: A MEKK-regulated JNK pathway activated by FcɛRI that regulates TNF-α production in mast cells is defined.
References
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Journal ArticleDOI
Two types of mouse helper T cell clone. III. Further differences in lymphokine synthesis between Th1 and Th2 clones revealed by RNA hybridization, functionally monospecific bioassays, and monoclonal antibodies.
TL;DR: Lymphokine synthesis patterns of a panel of 19 T cell clones have been evaluated, using mRNA hybridization methods to examine 11 different mRNAs induced by Con A, and it is shown that secreted protein and mRNA levels correlated well for all cell lines.
Journal Article
B cell stimulatory factor-1 enhances the IgE response of lipopolysaccharide-activated B cells.
TL;DR: Highly purified BSF-1 from a different source, the T lymphoma cell line EL-4, enhanced IgE production to the same extent as TH supernatants, which suggests that B SF-1 is responsible for this increase in IgEProduction.
Journal ArticleDOI
The role of helper T cell products in mouse B cell differentiation and isotype regulation.
Robert L. Coffman,Brian W. P. Seymour,Deborah A. Lebman,Debra D. Hiraki,Judy A. Christiansen,B Shrader,Holly M. Cherwinski,Huub F. J. Savelkoul,Fred D. Finkelman,Martha W. Bond,Tim R. Mosmann +10 more
TL;DR: In this article, the authors summarize the efforts to define the specific helper T cells or lymphokines which transmit regulatory signals to B cells, and to clarify the individual events influenced by these signals.
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