Journal ArticleDOI
Mast cell lines produce lymphokines in response to cross-linkage of Fc epsilon RI or to calcium ionophores.
Marshall Plaut,Jacalyn H. Pierce,Cynthia J. Watson,J. Hanley-Hyde,Richard P. Nordan,William E. Paul +5 more
Reads0
Chats0
TLDR
It is shown that cross-linkage of FcεRI on a series of non-transformed murine mast cell lines, or treatment of these cells with calcium ionophores, stimulates increased messenger RNA levels and secretion of a group of lymphokines classically produced by a subset of murine T cell lines (TH2cells).Abstract:
The cross-linkage of high affinity Fc epsilon receptors (Fc epsilon RI) on mast cells and basophils is central to the induction of allergic inflammatory responses. As a result of such cross-linkage, mast cells secrete a variety of preformed biologically active substances, such as histamine, and newly synthesized arachidonic acid metabolites. Here we show that cross-linkage of Fc epsilon RI on a series of nontransformed murine mast cell lines, or treatment of these cells with calcium ionophores, stimulates increased messenger RNA levels and secretion of a group of lymphokines classically produced by a subset of murine T cell lines (TH2 cells). These factors include interleukin-3 (a mast cell growth factor)s interleukin-4 (an IgE 'switch factor'), interleukin-5 (an eosinophil differentiation factor) and interleukin-6 (a factor controlling immunoglobulin secretion). The production of these polypeptide factors by mast cells may have great importance in the induction of allergic and anti-parasite inflammatory responses.read more
Citations
More filters
Journal ArticleDOI
Transcription factor GATA-3 is differentially expressed in murine Th1 and Th2 cells and controls Th2-specific expression of the interleukin-5 gene.
TL;DR: It is demonstrated that GATA-3 is critical for expression of the IL-5 gene in bona fide Th2 cells and has therapeutic potential in the treatment of asthma and other hypereosinophilic diseases.
Journal ArticleDOI
Myofibroblasts and Subepithelial Fibrosis in Bronchial Asthma
Charlotte E. P. Brewster,Peter H. Howarth,Ratko Djukanovic,John W Wilson,Stephen T. Holgate,William R. Roche +5 more
TL;DR: Researchers studied endobronchial biopsies from eight normal and seven asthmatic volunteers by immunohistochemistry and electron microscopy to determine the nature of the cell responsible for thickened bronchial epithelial basement membrane and found it to be myofibroblastic.
Book ChapterDOI
New Insights into “The Riddle of the Mast Cells”: Microenvironmental Regulation of Mast Cell Development and Phenotypic Heterogeneity
TL;DR: In virtually every facet of the mast cell’s natural history, from its differentiation and maturation to its biochemistry and function, recent work has revealed considerably more complexity than had previously been suspected to exist.
Book ChapterDOI
The kit ligand, stem cell factor
TL;DR: This work shows that SCF critically regulates the migration and survival of mast cell precursors and mast cell adherence, promotes the proliferation of both immature and mature mast cells, enhances mast cell maturation, and regulates the extent of mediator release in mast cells activated by immunoglobulin (IgE)-dependent mechanisms.
References
More filters
Journal ArticleDOI
Identification of a common nucleotide sequence in the 3'-untranslated region of mRNA molecules specifying inflammatory mediators
TL;DR: A 33-nucleotide sequence, comprised entirely of A and T residues and located in the 3'-untranslated region, is conserved in toto in the murine and human TNF mRNAs.
Journal ArticleDOI
Production of hybridoma growth factor by human monocytes.
TL;DR: Limiting dilution analysis of the producing cells in combination with size, density and adherence characteristics showed that HGF is produced by monocytes and not by lymphocytes, and there was no need for the monocytes to be stimulated but the cells did require the presence of serum.
Journal ArticleDOI
Two types of mouse helper T cell clone. III. Further differences in lymphokine synthesis between Th1 and Th2 clones revealed by RNA hybridization, functionally monospecific bioassays, and monoclonal antibodies.
TL;DR: Lymphokine synthesis patterns of a panel of 19 T cell clones have been evaluated, using mRNA hybridization methods to examine 11 different mRNAs induced by Con A, and it is shown that secreted protein and mRNA levels correlated well for all cell lines.
Journal Article
B cell stimulatory factor-1 enhances the IgE response of lipopolysaccharide-activated B cells.
TL;DR: Highly purified BSF-1 from a different source, the T lymphoma cell line EL-4, enhanced IgE production to the same extent as TH supernatants, which suggests that B SF-1 is responsible for this increase in IgEProduction.
Journal ArticleDOI
The role of helper T cell products in mouse B cell differentiation and isotype regulation.
Robert L. Coffman,Brian W. P. Seymour,Deborah A. Lebman,Debra D. Hiraki,Judy A. Christiansen,B Shrader,Holly M. Cherwinski,Huub F. J. Savelkoul,Fred D. Finkelman,Martha W. Bond,Tim R. Mosmann +10 more
TL;DR: In this article, the authors summarize the efforts to define the specific helper T cells or lymphokines which transmit regulatory signals to B cells, and to clarify the individual events influenced by these signals.
Related Papers (5)
Mast cells as a source of both preformed and immunologically inducible TNF-α/cachectin
John R. Gordon,Stephen J. Galli +1 more