Journal ArticleDOI
Mast cell lines produce lymphokines in response to cross-linkage of Fc epsilon RI or to calcium ionophores.
Marshall Plaut,Jacalyn H. Pierce,Cynthia J. Watson,J. Hanley-Hyde,Richard P. Nordan,William E. Paul +5 more
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TLDR
It is shown that cross-linkage of FcεRI on a series of non-transformed murine mast cell lines, or treatment of these cells with calcium ionophores, stimulates increased messenger RNA levels and secretion of a group of lymphokines classically produced by a subset of murine T cell lines (TH2cells).Abstract:
The cross-linkage of high affinity Fc epsilon receptors (Fc epsilon RI) on mast cells and basophils is central to the induction of allergic inflammatory responses. As a result of such cross-linkage, mast cells secrete a variety of preformed biologically active substances, such as histamine, and newly synthesized arachidonic acid metabolites. Here we show that cross-linkage of Fc epsilon RI on a series of nontransformed murine mast cell lines, or treatment of these cells with calcium ionophores, stimulates increased messenger RNA levels and secretion of a group of lymphokines classically produced by a subset of murine T cell lines (TH2 cells). These factors include interleukin-3 (a mast cell growth factor)s interleukin-4 (an IgE 'switch factor'), interleukin-5 (an eosinophil differentiation factor) and interleukin-6 (a factor controlling immunoglobulin secretion). The production of these polypeptide factors by mast cells may have great importance in the induction of allergic and anti-parasite inflammatory responses.read more
Citations
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Mast cells and histamine in asthma
TL;DR: Research concerning airway reactions triggered by mast cells is helping elucidate sentinel events in the pathogenesis of asthma, because these reactions can be carefully monitored and manipulated.
Journal ArticleDOI
Stimulation of Blood Mononuclear Cells of Atopic Children with the Relevant Allergen Induces the Release of Eosinophil Chemotaxins Such as IL-3, IL-5, and GM-CSF
TL;DR: PBMC activation with the relevant allergen induces the release by T cells with a Th2 phenotype of chemotactic factors for eosinophils, and anti-human (ah) IL-3, ahIL-5, and ahGM-CSF effectively decreased eos inophil chemotaxis.
Journal ArticleDOI
Interleukin-4 Receptor α Chain and STAT6 Signaling Inhibit Gamma Interferon but Not Th2 Cytokine Expression within Schistosome Granulomas
TL;DR: Conventional Th cells in Stat6 KO granulomas produce IFN-γ and Th2 cytokines, but is unnecessary for Th2-cell development or localization within the granuloma.
Journal ArticleDOI
Serosal mast cells maintain their viability and promote the metabolism of cartilage proteoglycans when cocultured with chondrocytes.
Richard L. Stevens,Richard L. Stevens,Richard L. Stevens,Laura L. Somerville,Duane Sewell,Duane Sewell,Duane Sewell,James R. Swafford,James R. Swafford,James R. Swafford,John P. Caulfield,John P. Caulfield,John P. Caulfield,Francesca Levi-Schaffer,Francesca Levi-Schaffer,Francesca Levi-Schaffer,John R. Hubbard,John R. Hubbard,John R. Hubbard,Elahe T. Dayton,Elahe T. Dayton,Elahe T. Dayton +21 more
TL;DR: It is indicated that a complex interaction occurs in which the nonactivated MC stimulates biosynthesis and the activated MC degrades cartilage proteoglycans.
Journal ArticleDOI
Inhibitory effect of heat-killed Lactobacillus strain on immunoglobulin E-mediated degranulation and late-phase immune reactions of mouse bone marrow-derived mast cells
TL;DR: Results suggest that some Lactobacillus strains inhibited IgE-mediated mast cell degranulation and subsequent late-phase reactions involving mast cells via a TLR2-dependent mechanism with FcεRIα downregulation.
References
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Journal ArticleDOI
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TL;DR: Lymphokine synthesis patterns of a panel of 19 T cell clones have been evaluated, using mRNA hybridization methods to examine 11 different mRNAs induced by Con A, and it is shown that secreted protein and mRNA levels correlated well for all cell lines.
Journal Article
B cell stimulatory factor-1 enhances the IgE response of lipopolysaccharide-activated B cells.
TL;DR: Highly purified BSF-1 from a different source, the T lymphoma cell line EL-4, enhanced IgE production to the same extent as TH supernatants, which suggests that B SF-1 is responsible for this increase in IgEProduction.
Journal ArticleDOI
The role of helper T cell products in mouse B cell differentiation and isotype regulation.
Robert L. Coffman,Brian W. P. Seymour,Deborah A. Lebman,Debra D. Hiraki,Judy A. Christiansen,B Shrader,Holly M. Cherwinski,Huub F. J. Savelkoul,Fred D. Finkelman,Martha W. Bond,Tim R. Mosmann +10 more
TL;DR: In this article, the authors summarize the efforts to define the specific helper T cells or lymphokines which transmit regulatory signals to B cells, and to clarify the individual events influenced by these signals.
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