Journal ArticleDOI
Measuring the metabolome: current analytical technologies
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TLDR
A review of mass spectrometry, NMR spectroscopy and vibrational spectroscopic techniques for the study of variations within the metabolome in many animal, plant and microbial systems discusses the advantages and disadvantages of each technique.Abstract:
The post-genomics era has brought with it ever increasing demands to observe and characterise variation within biological systems. This variation has been studied at the genomic (gene function), proteomic (protein regulation) and the metabolomic (small molecular weight metabolite) levels. Whilst genomics and proteomics are generally studied using microarrays (genomics) and 2D-gels or mass spectrometry (proteomics), the technique of choice is less obvious in the area of metabolomics. Much work has been published employing mass spectrometry, NMR spectroscopy and vibrational spectroscopic techniques, amongst others, for the study of variations within the metabolome in many animal, plant and microbial systems. This review discusses the advantages and disadvantages of each technique, putting the current status of the field of metabolomics in context, and providing examples of applications for each technique employed.read more
Citations
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Procedures for large-scale metabolic profiling of serum and plasma using gas chromatography and liquid chromatography coupled to mass spectrometry
Warwick B. Dunn,David Broadhurst,David Broadhurst,Paul Begley,Eva Zelena,Sue Francis-McIntyre,Nadine Anderson,Marie Brown,Joshau D Knowles,Antony Halsall,John N. Haselden,Andrew W. Nicholls,Ian D. Wilson,Douglas B. Kell,Royston Goodacre +14 more
TL;DR: The experimental workflow for long-term and large-scale metabolomic studies involving thousands of human samples with data acquired for multiple analytical batches over many months and years is described.
Journal ArticleDOI
Discovery and resupply of pharmacologically active plant-derived natural products: A review.
Atanas G. Atanasov,Birgit Waltenberger,Eva-Maria Pferschy-Wenzig,Thomas Linder,Christoph Wawrosch,Pavel Uhrin,Veronika Temml,Limei Wang,Stefan Schwaiger,Elke H. Heiss,Judith M. Rollinger,Judith M. Rollinger,Daniela Schuster,Johannes M. Breuss,Valery N. Bochkov,Marko D. Mihovilovic,Brigitte Kopp,Rudolf Bauer,Verena M. Dirsch,Hermann Stuppner +19 more
TL;DR: While the intrinsic complexity of natural product-based drug discovery necessitates highly integrated interdisciplinary approaches, the reviewed scientific developments, recent technological advances, and research trends clearly indicate that natural products will be among the most important sources of new drugs in the future.
Journal ArticleDOI
Hydrophilic interaction chromatography.
Petrus Hemström,Knut Irgum +1 more
TL;DR: The review attempts to summarize the ongoing discussion on the separation mechanism and gives an overview of the stationary phases used and the applications addressed with this separation mode in LC.
Journal ArticleDOI
Emerging applications of metabolomics in drug discovery and precision medicine
TL;DR: This Review discusses some of the latest technological advances in metabolomics, focusing on the application of metabolomics towards uncovering the underlying causes of complex diseases, the growing role of metabolites in drug discovery and its potential effect on precision medicine.
Journal ArticleDOI
The model organism as a system: integrating 'omics' data sets.
TL;DR: Researchers are rising to the challenge by using omics data integration to address fundamental biological questions that would increase the understanding of systems as a whole.
References
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