Methyl jasmonate and salicylic acid induced oxidative stress and accumulation of phenolics in Panax ginseng bioreactor root suspension cultures.
Reads0
Chats0
TLDR
Results strongly indicate that MJ and SA induce the accumulation of phenolic compounds in ginseng root by altering the phenolic synthesis enzymes.Abstract:
To investigate the enzyme variations responsible for the synthesis of phenolics, 40 day-old adventitious roots of Panax ginseng were treated with 200 µM methyl jasmonate (MJ) or salicylic acid (SA) in a 5 L bioreactor suspension culture (working volume 4 L). Both treatments caused an increase in the carbonyl and hydrogen peroxide (H 2 O 2 ) contents, although the levels were lower in SA treated roots. Total phenolic, flavonoid, ascorbic acid, non-protein thiol (NPSH) and cysteine contents and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical reducing activity were increased by MJ and SA. Fresh weight (FW) and dry weight (DW) decreased significantly after 9 days of exposure to SA and MJ. The highest total phenolics (62%), DPPH activity (40%), flavonoids (88%), ascorbic acid (55%), NPSH (33%), and cysteine (62%) contents compared to control were obtained after 9 days in SA treated roots. The activities of glucose 6-phosphate dehydrogenase, phenylalanine ammonia lyase, substrate specific peroxidases (caffeic acid peroxidase, quercetin peroxidase and ferulic acid peroxidase) were higher in MJ treated roots than the SA treated ones. Increased shikimate dehydrogenase, chlorogenic acid peroxidase and β-glucosidase activities and proline content were observed in SA treated roots than in MJ ones. Cinnamyl alcoholread more
Citations
More filters
Journal ArticleDOI
Salicylic acid-induced abiotic stress tolerance and underlying mechanisms in plants
TL;DR: The role of salicylic acid is critically appraised in plants exposed to major abiotic stresses and potential mechanisms potentially governing SA-induced plant abiotic stress-tolerance are discussed.
Journal ArticleDOI
Elicitation, an Effective Strategy for the Biotechnological Production of Bioactive High-Added Value Compounds in Plant Cell Factories.
Karla Ramirez-Estrada,Heriberto Vidal-Limon,Diego Hidalgo,Elisabeth Moyano,Marta Golenioswki,Rosa M. Cusidó,Javier Palazon +6 more
TL;DR: The enhancing effects of elicitors on the production of high-added value plant compounds such as taxanes, ginsenosides, aryltetralin lignans and other types of polyphenols are summarized, focusing particularly on the use of a new generation of elicitor such as coronatine and cyclodextrins.
Journal ArticleDOI
The ameliorative effects of exogenous melatonin on grape cuttings under water-deficient stress: antioxidant metabolites, leaf anatomy, and chloroplast morphology
TL;DR: It is concluded that the application of melatonin to wine grapes is effective in reducing drought stress.
Journal ArticleDOI
Recent advances in ginseng as cancer therapeutics: a functional and mechanistic overview
TL;DR: The mechanisms of action of ginsenosides and their metabolites, which can modulate signaling pathways associated with inflammation, oxidative stress, angiogenesis, metastasis, and stem/progenitor-like properties of cancer cells are reviewed.
Journal ArticleDOI
Production of biomass and useful compounds from adventitious roots of high-value added medicinal plants using bioreactor.
TL;DR: In this review, various physiological, engineering parameters, and selection of proper cultivation strategy affecting the biomass production and secondary metabolite accumulation have been discussed and advances in adventitious root cultures including factors for process scale-up as well as recent research aimed at maximizing automation of the bioreactor production processes are highlighted.
References
More filters
Journal ArticleDOI
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
Journal ArticleDOI
A revised medium for rapid growth and bio assays with tobacco tissue cultures
Toshio Murashige,Folke Skoog +1 more
TL;DR: In vivo redox biosensing resolves the spatiotemporal dynamics of compartmental responses to local ROS generation and provide a basis for understanding how compartment-specific redox dynamics may operate in retrograde signaling and stress 67 acclimation in plants.
Journal ArticleDOI
Tissue sulfhydryl groups
TL;DR: A water-soluble (at pH 8) aromatic disulfide [5,5′-dithiobis(2-nitrobenzoic acid] has been synthesized and shown to be useful for determination of sulfhydryl groups.
Journal ArticleDOI
Rapid determination of free proline for water-stress studies
TL;DR: In this article, a simple colorimetric determination of proline in the 0.1 to 36.0 μmoles/g range of fresh weight leaf material was presented.
Related Papers (5)
A revised medium for rapid growth and bio assays with tobacco tissue cultures
Toshio Murashige,Folke Skoog +1 more