Journal ArticleDOI
Microfabricated multilayer parylene‐C stencils for the generation of patterned dynamic co‐cultures
Satoshi Jinno,Hannes-Christian Moeller,Hannes-Christian Moeller,Chia-Ling Chen,Bimal Rajalingam,Bimal Rajalingam,Bong Geun Chung,Bong Geun Chung,Mehmet R. Dokmeci,Ali Khademhosseini,Ali Khademhosseini +10 more
TLDR
In this paper, a micropatterning technique based on microfabricated multilayer parylene-C stencils was proposed to generate dynamic co-cultures of murine embryonic stem cells.Abstract:
Co-culturing different cell types can be useful to engineer a more in vivo-like microenvironment for cells in culture. Recent approaches to generating cellular co-cultures have used microfabrication technologies to regulate the degree of cell-cell contact between different cell types. However, these approaches are often limited to the co-culture of only two cell types in static cultures. The dynamic aspect of cell-cell interaction, however, is a key regulator of many biological processes such as early development, stem cell differentiation, and tissue regeneration. In this study, we describe a micropatterning technique based on microfabricated multilayer parylene-C stencils and demonstrate the potential of parylene-C technology for co-patterning of proteins and cells with the ability to generate a series of at least five temporally controlled patterned co-cultures. We generated dynamic co-cultures of murine embryonic stem cells in culture with various secondary cell types that could be sequentially introduced and removed from the co-cultures. Our studies suggested that dynamic co-cultures generated by using parylene-C stencils may be applicable in studies investigating cellular interactions in controlled microenvironments such as studies of ES cell differentiation, wound healing and development.read more
Citations
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Journal ArticleDOI
Controlling Spatial Organization of Multiple Cell Types in Defined 3D Geometries
Halil Tekin,Jefferson G. Sanchez,Jefferson G. Sanchez,Christian Landeros,Christian Landeros,Karen Dubbin,Karen Dubbin,Robert Langer,Ali Khademhosseini,Ali Khademhosseini +9 more
TL;DR: A simple method to pattern multiple cell types in a spatially controlled manner within predefined microenvironments to replicate these intricate native organizations, which can lead to useful models for several fields.
Journal ArticleDOI
Template-based and template-free preparation of nanostructured parylene via oblique angle polymerization
TL;DR: In this paper, a comparative study of template-based and template-free methods for the deposition of parylene nanofibers is presented, which combine both vapor deposition polymerization and oblique angle deposition.
Journal ArticleDOI
Microengineering hydrogels for stem cell bioengineering and tissue regeneration
Ian Wheeldon,Amirhossein F. Ahari,Amirhossein F. Ahari,Ali Khademhosseini,Ali Khademhosseini +4 more
TL;DR: Techniques to develop hydrogel microstructures for controlling cell aggregate formation to regulate stem cell behavior as well as a bottom-up and a top-down microengineering approach to creating biomimic tissue-like structures are discussed.
Journal ArticleDOI
Engineered Tools to Study Intercellular Communication
TL;DR: In this article, a review discusses how salient engineered approaches and sequencing techniques can be applied to understand collective cell behavior and tissue functions, while resolving the molecular heterogeneity in "omic" layers that contribute to cell state and function.
Journal ArticleDOI
Advances and Challenges in Recapitulating Human Pulmonary Systems: At the Cusp of Biology and Materials
TL;DR: Current LoC platforms that have been used for testing the efficacy of drugs or as model systems for investigating disorders of the lung parenchyma are discussed and the design parameters in developing an ideal physiologically relevant LoC platform are presented.
References
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Journal ArticleDOI
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William C. Claycomb,Nicholas A. Lanson,Beverly S. Stallworth,Daniel B. Egeland,Joseph B. Delcarpio,Anthony Bahinski,Nicholas J. Izzo +6 more
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