Journal ArticleDOI
Microfabricated multilayer parylene‐C stencils for the generation of patterned dynamic co‐cultures
Satoshi Jinno,Hannes-Christian Moeller,Hannes-Christian Moeller,Chia-Ling Chen,Bimal Rajalingam,Bimal Rajalingam,Bong Geun Chung,Bong Geun Chung,Mehmet R. Dokmeci,Ali Khademhosseini,Ali Khademhosseini +10 more
TLDR
In this paper, a micropatterning technique based on microfabricated multilayer parylene-C stencils was proposed to generate dynamic co-cultures of murine embryonic stem cells.Abstract:
Co-culturing different cell types can be useful to engineer a more in vivo-like microenvironment for cells in culture. Recent approaches to generating cellular co-cultures have used microfabrication technologies to regulate the degree of cell-cell contact between different cell types. However, these approaches are often limited to the co-culture of only two cell types in static cultures. The dynamic aspect of cell-cell interaction, however, is a key regulator of many biological processes such as early development, stem cell differentiation, and tissue regeneration. In this study, we describe a micropatterning technique based on microfabricated multilayer parylene-C stencils and demonstrate the potential of parylene-C technology for co-patterning of proteins and cells with the ability to generate a series of at least five temporally controlled patterned co-cultures. We generated dynamic co-cultures of murine embryonic stem cells in culture with various secondary cell types that could be sequentially introduced and removed from the co-cultures. Our studies suggested that dynamic co-cultures generated by using parylene-C stencils may be applicable in studies investigating cellular interactions in controlled microenvironments such as studies of ES cell differentiation, wound healing and development.read more
Citations
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Journal ArticleDOI
Carbon nanofiber electrode array for the detection of lead
TL;DR: In this article, a nanoelectrode array of vertically aligned carbon nanofibers was evaluated for the detection of Pb 2 + by anodic stripping voltammetry.
Journal ArticleDOI
CC chemokine ligand 2 and leukemia inhibitory factor cooperatively promote pluripotency in mouse induced pluripotent cells.
Yuki Hasegawa,Naoko Takahashi,Alistair R. R. Forrest,Jay W. Shin,Yohei Kinoshita,Harukazu Suzuki,Yoshihide Hayashizaki +6 more
TL;DR: It is demonstrated that Ccl2‐mediated increased pluripotency is independent of phosphoinositide 3‐kinase and mitogen‐activated protein kinase pathways and that Tbx3 may be upregulated by Klf4.
Journal ArticleDOI
Microfluidic analysis of heterotypic cellular interactions: A review of techniques and applications
TL;DR: The categorization of recently developed in vitro organ and disease models based on the different microfluidic co-culture techniques will serve as a guideline for choosing the appropriate method for an intended cell-cell communication study.
Journal ArticleDOI
Addressable nanowell arrays formed using reversibly sealable hybrid elastomer-metal stencils.
TL;DR: An addressable nanoliter-well plate with micrometer sized wells that combines the advantages of the two array formats used in life science research and biomedical analysis is presented.
Patent
Method of cryopreservation of stem cell-derived retinal pigment epithelial cells on polymeric substrate
TL;DR: In this paper, methods and compositions for the cryopreservation of stem cells, such as stem-cell derived retinal pigment epithelial cells, have been seeded onto and cultured on a substrate.
References
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Journal ArticleDOI
HL-1 cells: A cardiac muscle cell line that contracts and retains phenotypic characteristics of the adult cardiomyocyte
William C. Claycomb,Nicholas A. Lanson,Beverly S. Stallworth,Daniel B. Egeland,Joseph B. Delcarpio,Anthony Bahinski,Nicholas J. Izzo +6 more
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