Journal ArticleDOI
Microfabricated multilayer parylene‐C stencils for the generation of patterned dynamic co‐cultures
Satoshi Jinno,Hannes-Christian Moeller,Hannes-Christian Moeller,Chia-Ling Chen,Bimal Rajalingam,Bimal Rajalingam,Bong Geun Chung,Bong Geun Chung,Mehmet R. Dokmeci,Ali Khademhosseini,Ali Khademhosseini +10 more
TLDR
In this paper, a micropatterning technique based on microfabricated multilayer parylene-C stencils was proposed to generate dynamic co-cultures of murine embryonic stem cells.Abstract:
Co-culturing different cell types can be useful to engineer a more in vivo-like microenvironment for cells in culture. Recent approaches to generating cellular co-cultures have used microfabrication technologies to regulate the degree of cell-cell contact between different cell types. However, these approaches are often limited to the co-culture of only two cell types in static cultures. The dynamic aspect of cell-cell interaction, however, is a key regulator of many biological processes such as early development, stem cell differentiation, and tissue regeneration. In this study, we describe a micropatterning technique based on microfabricated multilayer parylene-C stencils and demonstrate the potential of parylene-C technology for co-patterning of proteins and cells with the ability to generate a series of at least five temporally controlled patterned co-cultures. We generated dynamic co-cultures of murine embryonic stem cells in culture with various secondary cell types that could be sequentially introduced and removed from the co-cultures. Our studies suggested that dynamic co-cultures generated by using parylene-C stencils may be applicable in studies investigating cellular interactions in controlled microenvironments such as studies of ES cell differentiation, wound healing and development.read more
Citations
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Journal ArticleDOI
Differential regulation of morphology and stemness of mouse embryonic stem cells by substrate stiffness and topography.
TL;DR: A micro-fabricated polyacrylamide hydrogel substrate with two elasticities and three topographies was applied to systematically test the morphology, proliferation, and stemness of mESCs and the independent or combined impact of the two factors on specific cell functions was analyzed.
Journal ArticleDOI
Micropatterned superhydrophobic structures for the simultaneous culture of multiple cell types and the study of cell-cell communication.
Alexander N. Efremov,Eliana Stanganello,Alexander Welle,Steffen Scholpp,Pavel A. Levkin,Pavel A. Levkin +5 more
TL;DR: A convenient method for creating patterns of multiple (up to twenty) different cell types on one substrate and the cross-talk between two cell populations via Wnt signaling molecules propagation during co-culture in a mutual culture medium is shown.
Journal ArticleDOI
Resistless nanofabrication by stencil lithography
TL;DR: In this article, a review of stencil lithography in nanoscale patterning for the direct deposition of complex materials and the patterning on non-conventional substrates is presented.
Journal ArticleDOI
Manipulation of Signaling Thresholds in ``Engineered Stem Cell Niches'' Identifies Design Criteria for Pluripotent Stem Cell Screens
TL;DR: Model results and empirical observations demonstrate that colonies less than 100 µm in diameter are too small to maximize endogenous Stat3 activation and that colonies separated by more than 400 µm can be considered independent from each other, and suggest that niche size is an important parameter in stem cell fate control.
Journal ArticleDOI
Nanoscale Resolution, Multicomponent Biomolecular Arrays Generated By Aligned Printing With Parylene Peel-Off
TL;DR: This work presents "Print-and-Peel", a high-throughput method to generate multicomponent biomolecular arrays with sub-100 nm nanoscale feature width by performing a second print-run superimposed over the first, thereby extending the multiplexing capability of the technique.
References
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Journal ArticleDOI
HL-1 cells: A cardiac muscle cell line that contracts and retains phenotypic characteristics of the adult cardiomyocyte
William C. Claycomb,Nicholas A. Lanson,Beverly S. Stallworth,Daniel B. Egeland,Joseph B. Delcarpio,Anthony Bahinski,Nicholas J. Izzo +6 more
TL;DR: In this paper, the authors derived a cardiac muscle cell line, designated HL-1, from the AT-1 mouse atrial cardiomyocyte tumor lineage, which can be serially passaged, yet they maintain the ability to contract and retain differentiated cardiac morphological, biochemical, and electrophysiological properties.