miRNAs as Predictive Factors in Early Diagnosis of Gestational Diabetes Mellitus
Ilona Juchnicka,Mariusz Kuzmicki,Magdalena Niemira,Agnieszka Bielska,Iwona Sidorkiewicz,Monika Zbucka-Kretowska,Adam Kretowski,Jacek Szamatowicz +7 more
Reads0
Chats0
TLDR
The role of miRNAs as a predictive factor that could potentially be useful in early diagnosis of gestational diabetes mellitus is emphasized, and changes in miRNA profile in the serum of GDM women may indicate significance in the pathophysiology of G DM.Abstract:
Introduction Circulating miRNAs are important mediators in epigenetic changes. These non-coding molecules regulate post-transcriptional gene expression by binding to mRNA. As a result, they influence the development of many diseases, such as gestational diabetes mellitus (GDM). Therefore, this study investigates the changes in the miRNA profile in GDM patients before hyperglycemia appears. Materials and Methods The study group consisted of 24 patients with GDM, and the control group was 24 normoglycemic pregnant women who were matched for body mass index (BMI), age, and gestational age. GDM was diagnosed with an oral glucose tolerance test between the 24th and 26th weeks of pregnancy. The study had a prospective design, and serum for analysis was obtained in the first trimester of pregnancy. Circulating miRNAs were measured using the NanoString quantitative assay platform. Validation with real time-polymerase chain reaction (RT-PCR) was performed on the same group of patients. Mann-Whitney U-test and Spearman correlation were done to assess the significance of the results. Results Among the 800 miRNAs, 221 miRNAs were not detected, and 439 were close to background noise. The remaining miRNAs were carefully investigated for their average counts, fold changes, p-values, and false discovery rate (FDR) scores. We selected four miRNAs for further validation: miR-16-5p, miR-142-3p, miR-144-3p, and miR-320e, which showed the most prominent changes between the studied groups. The validation showed up-regulation of miR-16-5p (p<0.0001), miR-142-3p (p=0.001), and miR-144-3p (p=0.003). Conclusion We present changes in miRNA profile in the serum of GDM women, which may indicate significance in the pathophysiology of GDM. These findings emphasize the role of miRNAs as a predictive factor that could potentially be useful in early diagnosis.read more
Citations
More filters
Journal ArticleDOI
Cardiovascular Disease-Associated MicroRNAs as Novel Biomarkers of First-Trimester Screening for Gestational Diabetes Mellitus in the Absence of Other Pregnancy-Related Complications
TL;DR: Cardiovascular disease-associated microRNAs represent promising early biomarkers to be implemented into routine first-trimester screening programs with a very good predictive potential for gestational diabetes mellitus.
Journal ArticleDOI
Milk Exosomal microRNAs: Postnatal Promoters of β Cell Proliferation but Potential Inducers of β Cell De-Differentiation in Adult Life
Bodo C. Melnik,Gerd Schmitz +1 more
TL;DR: Mechanistic evidence suggests that MEX miRs stimulate mTORC1/c-MYC-dependent postnatal β cell proliferation and glycolysis, but attenuate β cell differentiation, mitochondrial function, and insulin synthesis and secretion, while persistent bovine MEX exposure after the lactation period may de-differentiate β cells back to the postnatal phenotype.
Journal ArticleDOI
The Role of microRNAs in Inflammation
Kaushik Das,L. Vijaya Mohan Rao +1 more
TL;DR: The role of microRNAs in the development and progression of various inflammatory-associated diseases has gained much attention in the past two decades for their regulation of inflammatory gene expression at multiple levels and their potential to serve as biomarkers and therapeutic targets as mentioned in this paper .
Journal ArticleDOI
Deep Insight of the Pathophysiology of Gestational Diabetes Mellitus
Amarish Kumar Sharma,Sanjeev Singh,Himanshu Singh,Deviyani Mahajan,Prachetha Kolli,Gowtham Mandadapu,Bimlesh Kumar,Dharmendra Kumar,Sudarshan Kumar,Manoj Kumar Jena +9 more
TL;DR: The early diagnosis of the metabolic disorder, most preferably in first trimester of pregnancy, will lead to its effective long-term management, reducing foetal developmental complications and mortality along with safety measures for the mother.
Journal ArticleDOI
Systematic review of transcriptome and microRNAome associations with gestational diabetes mellitus
Kimberly A. Lewis,Lisa Chang,Julinna Cheung,Bradley E. Aouizerat,Laura L. Jelliffe-Pawlowski,Monica R. McLemore,Brian D. Piening,Larry Rand,Kelli K. Ryckman,Elena Flowers +9 more
TL;DR: In this article , a systematic review was conducted using the Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines to summarize the state of the science about changes in gene expression and Gestational Diabetes (GDM).
References
More filters
Journal ArticleDOI
MicroRNAs: small RNAs with a big role in gene regulation
Lin He,Gregory J. Hannon +1 more
TL;DR: Two founding members of the microRNA family were originally identified in Caenorhabditis elegans as genes that were required for the timed regulation of developmental events and indicate the existence of multiple RISCs that carry out related but specific biological functions.
Journal ArticleDOI
Prediction of Mammalian MicroRNA Targets
TL;DR: The predicted regulatory targets of mammalian miRNAs were enriched for genes involved in transcriptional regulation but also encompassed an unexpectedly broad range of other functions.
Journal ArticleDOI
Characterization of microRNAs in serum: a novel class of biomarkers for diagnosis of cancer and other diseases
Xi Chen,Yi Ba,Lijia Ma,Lijia Ma,Xing Cai,Yuan Yin,Kehui Wang,Jigang Guo,Yujing Zhang,Jiangning Chen,Xing Guo,Qibin Li,Qibin Li,Xiaoying Li,Wenjing Wang,Yan Zhang,Jin Wang,Xueyuan Jiang,Yang Xiang,Chen Xu,Pingping Zheng,Juanbin Zhang,Ruiqiang Li,Hongjie Zhang,Xiaobin Shang,Ting Gong,Guang Ning,Jun Wang,Jun Wang,Ke Zen,Junfeng Zhang,Chen-Yu Zhang +31 more
TL;DR: It is demonstrated that miRNAs are present in the serum and plasma of humans and other animals such as mice, rats, bovine fetuses, calves, and horses, and can serve as potential biomarkers for the detection of various cancers and other diseases.
Journal ArticleDOI
Causes and consequences of microRNA dysregulation in cancer
TL;DR: Because malignant cells show dependence on the dysregulated expression of miRNA genes, which in turn control or are controlled by the dysregulation of multiple protein-coding oncogenes or tumour suppressor genes, these small RNAs provide important opportunities for the development of future miRNA-based therapies.
Journal ArticleDOI
MicroRNAs in cancer.
TL;DR: In this paper, the effects of miRNA dysregulation in the cellular pathways that lead to the progressive conversion of normal cells into cancer cells and the potential to develop new molecular miRNA-targeted therapies are discussed.