Journal ArticleDOI
Molecular cloning of the receptor for human antidiuretic hormone.
Mariel Birnbaumer,Anita Seibold,S. Gilbert,Masami Ishido,Masami Ishido,Claude Barberis,Claude Barberis,Anaid Antaramian,Phillippe Brabet,Walter Rosenthal +9 more
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TLDR
The deduced amino-acid sequence of the receptor yields a hydropathy profile characteristic of receptors with seven putative transmembrane regions, which indicates that the ADH receptor is a member of the superfamily of G-protein-coupled receptors.Abstract:
ANTIDIURESIS, the recovery of water from the lumen of the renal collecting tubule, is regulated by the hypothalamic release of antidiuretic hormone (ADH), which binds to specific receptors on renal collecting tubule cells, stimulates adenylyl cyclase and promotes the cyclic AMP-mediated incorporation of water pores into the luminal surface of these cells'1–3. We report here the isolation of the human ADH receptor gene using a genomic expression cloning approach4. The gene was used to clone the complementary DNA from a human renal library. The deduced amino-acid sequence of the receptor yields a hydropathy profile characteristic of receptors with seven putative transmembrane regions. This and the comparison with other cloned receptors indicates that the ADH receptor is a member of the superfamily of G-protein-coupled receptors.read more
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A human gene that shows identity with the gene encoding the angiotensin receptor is located on chromosome 11
Brian F. O'Dowd,Michael Heiber,Audrey Chan,Henry H.Q. Heng,Lap-Chee Tsui,James L. Kennedy,Xiao-Mei Shi,Arturas Petronis,Susan R. George,Tuan V. Nguyen +9 more
TL;DR: PCR analysis of somatic cell lines found APJ-related sequences to be only present on chromosome 11, and high-resolution mapping by fluorescence in situ hybridization (FISH) sublocalized APJ on band q12.
Journal ArticleDOI
Gα15 and Gα16 Couple a Wide Variety of Receptors to Phospholipase C
TL;DR: These data demonstrate that Gα15 and Gα16 are unique in that they can be activated by a wide variety of G-protein-coupled receptors and can be a useful tool to understand the mechanism of receptor-induced G- protein activation.
Journal ArticleDOI
Nephrogenic diabetes insipidus.
TL;DR: The advances described here are examples of "bedside physiology" and provide diagnostic tools for physicians caring for patients with congenital nephrogenic diabetes insipidus and the acquired form of NDI, which is much more common than the congenital form, and is associated with downregulation of AQP2.
Journal ArticleDOI
Neuroendocrine Control of Body Fluid Metabolism
José Antunes-Rodrigues,Margaret de Castro,Lucila Leico Kagohara Elias,Marcelo Moraes Valença,Samuel M. McCann +4 more
TL;DR: The role played by brain ANP and its interaction with neurohypophysial hormones in the control of body fluid homeostasis is emphasized.
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Oxytocin and Vasopressin Agonists and Antagonists as Research Tools and Potential Therapeutics
Maurice Manning,Aleksandra Misicka,Aleksandra Misicka,Aleksandra Olma,Aleksandra Olma,K. Bankowski,Stoytcho Stoev,Bice Chini,Thierry Durroux,Bernard Mouillac,Maithé Corbani,Gilles Guillon +11 more
TL;DR: The status of peptide and non-peptide agonists and antagonists for the V1a, V1b and V2 receptors for arginine vasopressin (AVP) and the O2 receptor for oxytocin (OT) was reviewed in this paper.
References
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A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity
TL;DR: A technique for conveniently radiolabeling DNA restriction endonuclease fragments to high specific activity is described, and these "oligolabeled" DNA fragments serve as efficient probes in filter hybridization experiments.
Journal ArticleDOI
A simple method for displaying the hydropathic character of a protein
Jack Kyte,Russell F. Doolittle +1 more
TL;DR: A computer program that progressively evaluates the hydrophilicity and hydrophobicity of a protein along its amino acid sequence has been devised and its simplicity and its graphic nature make it a very useful tool for the evaluation of protein structures.
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity
TL;DR: In this article, a technique for conveniently radiolabeling DNA restriction endonuclease fragments to high specific activity is described, where DNA fragments are purified from agarose gels directly by ethanol precipitation and are then denatured and labeled with the large fragment of DNA polymerase I, using random oligonucleotides as primers.
Journal ArticleDOI
The scanning model for translation: an update.
TL;DR: The small (40S) subunit of eukaryotic ribosomes is believed to bind initially at the capped 5'-end of messenger RNA and then migrate, stopping at the first AUG codon in a favorable context for initiating translation.